Catalytic Synthesis and Characterization of Biodegradable ...

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Chapter 1 Figure 1.5.9 (A) SEM image of PANi-gelatin blend fibers with ratio 45:55. Original magnification is 5000×. (B) Morphology of H9c2 myoblast cells at 20 h post-seeding on 45:55 PANi-gelatin blend fiber. Staining is for nuclei-bisbenzimide and actin cytoskeletonphalloidin; fibers autofluoresce. Original magnification is 400×. (C) SEM images of H9c2 cells cultured on 45:55 PANI-gelatin blend fibers. 134 Despite the progress of PNAi in tissue engineering application, there was still a lot of work to do to improve their poor solubility, the poor polymer-cell interaction and biodegradability. Therefore, it is necessary to design novel electroactive polymers with good solubility, biocompatibility and biodegradability for the tissue engineering application. For example, polyanline was blended with natural polymers such as collagen and gelatin or covalently grafted with oligopeptides such as Ty-Ile-Gly-Ser-Arg (YIGSR) to improve its polymer-cell 133, 134 interaction (Figure 1.5.9). Figure 1.5.10 (A) Phase contrast images of PC-12 cell morphology of (a) TCP, (b) TCP with NGF, (c) ATQD-RGD,and (d) ATQD-RGD with NGF on day 10 (B)Neurite length distribution chart for ATQD-RGD substrates with and without NGF. ‐ 28 ‐
- 29 - Polymerization and Applications of Biodegradable Polyesters The solubility of polyaniline could be enhanced by covalently grafting side groups or polymers, such as poly(ethylene glycol), on the backbone of polyaniline. 135 Wei et al. 136 demonstrated that the electroactive silsesquioxane precursor, N-(4-aminophenyl)-N'-(4'-(3-triethoxysilyl-propyl-ureido) phenyl-1,4- quinonenediimine) (ATQD), containing aniline trimer covalently modified by oligopeptide could be a kind of promising biomaterial for tissue engineering. The bioactive material, ATQD-RGD, supported PC-12 cell adhesion and proliferation, and stimulated spontaneous neuritogenesis in PC-12 cells in the absence of neurotrophic growth factors (NGF), as shown in Figure 1.5.10. Figure 1.5.11 (A) Representational fluorescence micrographs of PC-12 cell for the substrates (a) TCPS (-) without electrical stimulation, (b) TCPS (+) exposed to electrical stimulation, (c) EM PLAAP (-) doped with CSA without electrical stimulation, (d) EM PLAAP (+) doped with CSA exposed to electrical stimulation on day 4. (B) The mean neurite length of PC-12 cells cultured on the substrates of EM PLAAP (-), TCPS (+), and EM PLAAP (+) on day 4. 136 Based on the above works, Chen’s group did many works to expand the architecture of this kind of materials. They chose aniline oligomers (especially aniline pentamer with dicarboxyl end group (AP)) as electroactivity resource, which incorporated with degradable polymers, such as polylactide (PLA), poly(ε-caprolactone) (PCL) and natural biopolymer chitosan, to prepare new biodegradable electroactive biomaterials. 137-139 The introducing of PLA endowed the material with good electroactivity, solubility and biodegradability similar to pure PLA. In vitro cell evaluation showed that the electroactive copolymers could indeed promote the attachment and growth of rat C6 glioma cells. Moreover, in the comparison
Page 1 and 2: Catalytic Synthesis and Characteriz
Page 3 and 4: Preface Biodegradable polyesters ha
Page 5 and 6: Chapter 3 Polymerization of Lactic
Page 7 and 8: Chapter 1 Polymerization and Applic
Page 9 and 10: - 3 - Polymerization and Applicatio
Page 13 and 14: 1.2.2 Aluminum Catalysts - 7 - Poly
Page 17 and 18: 1.3 Polylactide 1.3.1 Introduction
Page 19 and 20: - 13 - Polymerization and Applicati
Page 21 and 22: 1.3.4 Application of PLA Figure 1.3
Page 27 and 28: 1.5.1.4 Polyaniline (PANi) - 21 - P
Page 33: - 27 - Polymerization and Applicati
Page 47 and 48: 1.6.1 Radical Polymers for Organic
Page 51 and 52: initialization of the device by app
Page 55 and 56: 1.6.2.4 Others - 49 - Polymerizatio
Page 63 and 64: 182. R. B. Weller, J. Invest. Derma
Page 65 and 66: Chapter 2 Synthesis and Electrochem
Page 67 and 68: Synthesis and Electrochemistry of S
Page 79: Synthesis and Electrochemistry of S
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Chapter 3 3.1 Introduction Poly(lac
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Chapter 3 polydispersity index (PDI
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Chapter 3 aluminum based complexes
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Chapter 3 3.5 Conclusions Co(III) c
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Chapter 3 22. Z. H. Tang, X. S. Che
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Chapter 4 4.1 Introduction Stimulus
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Chapter 4 the proton of methenyl gr
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Chapter 4 attributed to the amide g
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Chapter 4 4.4 Thermal Properties Th
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Chapter 4 Figure 4.8 MTT assay for
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Chapter 4 The 1 H NMR and 13 C NMR
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Chapter 4 Cell viability (%) = (Asa
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Chapter 5 Synthesis of Triblock Cop
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5.2 Synthesis of Triblock Copolymer
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Sample Table 1. Feed and result com
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= 25 μm. B: MTT assay for the cyto
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‐ 109 ‐ Synthesis of Triblock C
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‐ 111 ‐ Synthesis of Triblock C
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Chapter 6 6.1 Introduction Since th
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Chapter 6 6.3 Synthesis of MPEG-b-P
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Chapter 6 used to characterize the
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Chapter 6 Figure 6.6 Cyclic voltamm
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Chapter 6 vivo cytotoxicity was als
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Chapter 6 NMR. Characterization of
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Chapter 6 CO2 for 24 h. RSC96 cells
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Chapter 7 Conclusion and Future Pro
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‐ 131 ‐ Conclusion and Future P
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‐ 133 ‐ Conclusion and Future P
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13. Xuan Pang, Xuesi Chen, Xiuli Zh
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Acknowledgments The present thesis
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