Catalytic Synthesis and Characterization of Biodegradable ...

Chapter 6
CO2 for 24 h. RSC96 cells were seeded at 1.2 ×104 cells/well in 96-well plates and cultured
for 24 h. Then the culture media were replaced by the polymer solutions at various diluted
concentrations. After incubation for additional 24 h, 20 μL of
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution (5 mg/mL)
was added to each well. The plates were return to the incubator for a period of 4 h culture.
The supernatant were then carefully removed from each well, and 200 μL DMSO was added
to dissolve the MTT formazan crystals. The plate was incubated for an additional 10 min
before the absorbance at 492 nm was recorded by an ELISA microplate reader (Bio-Rad).
The cell viability (%) was calculated according to the following equation:
Cell viability (%) = (Asample / Acontrol) × 100 (1)
Where Asample was the absorbance of the polymers extracted solutions treated cells and
Acontrol was the absorbance of the untreated cells. Each experiment was done in triplicate and
repeated a minimum of three times.
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