Catalytic Synthesis and Characterization of Biodegradable ...

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Synthesis of Lactide‐Grafted Poly(TEMPO‐acrylamide) and
its Electrochemical Property and Biocompatibility
Figure 4.9 Cell morphology of F-actin distribution in RSC96 cells incubated on cover-slip coated
with PLA (A), PTAm-g-PLA (B) and PTAm (C). RSC96 cells were incubated for 48 h and stained
for F-actin. Representative fluorescence microscopy photographs were shown here.
4.8 Conclusions
Novel PTAm-g-PLA copolymers were successfully prepared by a PLA macromonomer
approach. The obtained copolymer contained stable radical units which was biologically
active. The copolymers were amorphous which were determined from DSC measurement due
to the relatively low PLA content. The copolymer exhibited an improved biocompatibility
compared to PTAm homopolymer, which indicated that it was a promising biomaterial for the
tissue engineering.
4.9 Experimental Section
Materials and Methods
4-Amino-2,2,6,6-tetramethylpiperidine (>97%, TCI), hydroquinone (sinopharm chemical
reagent, >98%), 3-chloroperoxybenzonic acid (sinopharm chemical reagent, >85%) were
used as received. Acryloyl chloride was purchased from Best Chemical (China) and purified
by vacuum distillation. 2-Hydroxyethyl methacrylate (HEMA, 96%, Acros) was distilled
under reduced pressure before use. L-Lactide (LA) was purchased from Purac and
recrystallized in ethyl acetate three times. 2,2'-Azoisobutyronitrile (AIBN, Beijing Chemical
Co., China) was recrystallized twice from methanol. All the organic solvents used were dried
over CaH2 and distilled prior to use.