Catalytic Synthesis and Characterization of Biodegradable ...

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Chapter 4 Figure 4.8 MTT assay for the cytotoxicity of PLA-HEMA, PTAm and PTAm-g-PLA. Statistical significance: *p < 0.05 4.7 Cell Adhesion and Spreading Biocompatibility is also a great concern for medical applications. The biocompatibility of PTAm-g-PLA copolymers was evaluated by observing adhesion and spreading of RSC96 cells on copolymer films. Figure 4.9 showed the micrographs of RSC96 cells incubated for 48 h on PLA, PTAm-g-PLA2 and PTAm films, respectively. After 48 h, almost all cells adhered to the PLA and PTAm-g-PLA2 films and got spread. The cells extended from original elliptical shape to the irregular polygonal shape. Moreover, the filopodias appeared on the surface of cells and networks were formed among cells. All the above observation demonstrated that the adhesion and spreading of RSC96 cells on the PTAm-g-PLA2 were good. On the pure PTAm film, the cells also adhered well but their shapes were still original elliptical shape, which showed the cells did not spread on the film. The cells are separated and few filopodias or networks were observed. After copolymerized with PLA, the copolymers had improved biocompatibility compared with PTAm, which were comparable with PLA, indicating great potential use in tissue engineering. ‐ 94 ‐
‐ 95 ‐ Synthesis of Lactide‐Grafted Poly(TEMPO‐acrylamide) and its Electrochemical Property and Biocompatibility Figure 4.9 Cell morphology of F-actin distribution in RSC96 cells incubated on cover-slip coated with PLA (A), PTAm-g-PLA (B) and PTAm (C). RSC96 cells were incubated for 48 h and stained for F-actin. Representative fluorescence microscopy photographs were shown here. 4.8 Conclusions Novel PTAm-g-PLA copolymers were successfully prepared by a PLA macromonomer approach. The obtained copolymer contained stable radical units which was biologically active. The copolymers were amorphous which were determined from DSC measurement due to the relatively low PLA content. The copolymer exhibited an improved biocompatibility compared to PTAm homopolymer, which indicated that it was a promising biomaterial for the tissue engineering. 4.9 Experimental Section Materials and Methods 4-Amino-2,2,6,6-tetramethylpiperidine (>97%, TCI), hydroquinone (sinopharm chemical reagent, >98%), 3-chloroperoxybenzonic acid (sinopharm chemical reagent, >85%) were used as received. Acryloyl chloride was purchased from Best Chemical (China) and purified by vacuum distillation. 2-Hydroxyethyl methacrylate (HEMA, 96%, Acros) was distilled under reduced pressure before use. L-Lactide (LA) was purchased from Purac and recrystallized in ethyl acetate three times. 2,2'-Azoisobutyronitrile (AIBN, Beijing Chemical Co., China) was recrystallized twice from methanol. All the organic solvents used were dried over CaH2 and distilled prior to use.
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Catalytic Synthesis and Characteriz
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Preface Biodegradable polyesters ha
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Chapter 3 Polymerization of Lactic
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Chapter 1 Polymerization and Applic
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- 3 - Polymerization and Applicatio
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1.2.2 Aluminum Catalysts - 7 - Poly
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1.3 Polylactide 1.3.1 Introduction
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- 13 - Polymerization and Applicati
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1.3.4 Application of PLA Figure 1.3
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1.5.1.4 Polyaniline (PANi) - 21 - P
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1.6.1 Radical Polymers for Organic
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Page 63 and 64: 182. R. B. Weller, J. Invest. Derma
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Page 67 and 68: Synthesis and Electrochemistry of S
Page 79: Synthesis and Electrochemistry of S
Page 84 and 85: Chapter 3 3.1 Introduction Poly(lac
Page 86 and 87: Chapter 3 polydispersity index (PDI
Page 88 and 89: Chapter 3 aluminum based complexes
Page 90 and 91: Chapter 3 3.5 Conclusions Co(III) c
Page 92 and 93: Chapter 3 22. Z. H. Tang, X. S. Che
Page 94 and 95: Chapter 4 4.1 Introduction Stimulus
Page 96 and 97: Chapter 4 the proton of methenyl gr
Page 98 and 99: Chapter 4 attributed to the amide g
Page 100 and 101: Chapter 4 4.4 Thermal Properties Th
Page 104 and 105: Chapter 4 The 1 H NMR and 13 C NMR
Page 106 and 107: Chapter 4 Cell viability (%) = (Asa
Page 109 and 110: Chapter 5 Synthesis of Triblock Cop
Page 111 and 112: 5.2 Synthesis of Triblock Copolymer
Page 113 and 114: Sample Table 1. Feed and result com
Page 115 and 116: = 25 μm. B: MTT assay for the cyto
Page 117 and 118: ‐ 109 ‐ Synthesis of Triblock C
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Page 124 and 125: Chapter 6 6.1 Introduction Since th
Page 126 and 127: Chapter 6 6.3 Synthesis of MPEG-b-P
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Page 130 and 131: Chapter 6 Figure 6.6 Cyclic voltamm
Page 132 and 133: Chapter 6 vivo cytotoxicity was als
Page 134 and 135: Chapter 6 NMR. Characterization of
Page 136 and 137: Chapter 6 CO2 for 24 h. RSC96 cells
Page 141 and 142: Chapter 7 Conclusion and Future Pro
Page 143 and 144: ‐ 131 ‐ Conclusion and Future P
Page 145 and 146: ‐ 133 ‐ Conclusion and Future P
Page 147 and 148: 13. Xuan Pang, Xuesi Chen, Xiuli Zh
Page 149: Acknowledgments The present thesis
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