Application Compendium - Agilent Technologies

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Author Greg Saunders, Ben MacCreath Agilent Technologies, Inc. Size Exclusion Chromatography for the Analysis of Dental Polymers Application Note Introduction Copolymers of vinylmethyl ether and maleic anhydride are widely used in a range of dental applications. These include their use as a denture bioadhesive, as well as a toothpaste additive which helps the toothpaste to remain active between brushing. Batch to batch variations of such copolymer systems strongly influence performance in dental applications. Here, two batches of a commercial copolymer were analyzed by size exclusion chromatography (SEC). One of them had worked well in formulation but the other had failed.
Materials and Methods A column set comprising 2 x Agilent PL aquagel-OH MIXED 8 μm columns were used for the analysis. These versatile columns offer resolution over a wide range of molecular weight (up to 10,000,000 relative to PEG/PEO), simplifying column selection. Column calibration was achieved using Agilent EasiVial PEG/PEO standards. EasiVials provide a rapid and convenient means of constructing an aqueous SEC column calibration curve over a wide molecular weight range (typically 100 to 1,200,000 g/mol). The samples were made up in sodium nitrate buffer at neutral pH. Conditions Samples: Two samples of dental polymers Columns: 2 x PL aquagel-OH MIXED 8 µm, 300 x 7.5 mm (p/n PL1149-6800) Eluent: 0.2 M NaNO + 0.01 M NaH PO at pH 7 3 2 4 Flow Rate: 1.0 mL/min Detection: Agilent PL-GPC 50 Plus (DRI) Results and Discussion Figure 1 shows the Agilent EasiVial PEG/PEO calibration curve. Figure 2 shows overlaid raw data chromatograms for the two batches, indicating large differences in molecular weight and molecular weight distribution. 7 log M 2 10 min Figure 1. SEC PEG/PEO calibration using EasiVial standards 20 2 A B 5 min 20 Figure 2. Raw data chromatograms of two dental polymers The molecular weight distribution plots are presented in Figure 3 and clearly indicate the differences between the two batches. dw/dlog M 3 log M Figure 3. Overlay of the molecular weight distributions of two dental polymers Table 1. Mp, Mw, Mn and polydispersity values for the two dental polymers Batch Mp Mw Mn Polydispersity A 169,330 100,070 258,200 2.6 B 124,100 69,550 152,060 2.2 6
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MATERIALS TESTING AND RESEARCH SOLU
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When accuracy and reliability in me
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AGILENT TECHNOLOGIES APPLICATIONS F
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Figure 1. Agilent Cary 630 FTIR spe
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The calibration samples were measur
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Author Frank Higgins Agilent Techno
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Figure 1. The overlaid aliphatic be
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Author John Seelenbinder Agilent Te
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Figure 3. Sample is lightly pressed
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Author Dr. Mustafa Kansiz Agilent T
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microscopy provides for a factor of
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Standard ATR IR Image No Pressure (
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A visual inspection of the sample u
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Experimental Instrumentation To per
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Authors Jonah Kirkwood † and Must
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Spectra were collected from each lo
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Infrared mapping allows for multipl
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The spectra in Figure 2 are visuall
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Conclusion Agilent‟s Cary 610 FTI
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Authors Dr. Wayne Collins*, John Se
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The calibration curve obtained for
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Conclusion Select ‘Peak Ratio’
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Summary This method describes a pro
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Method configuration To determine t
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The calibration curve for the deter
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Figure 6. The MicroLab PC FTIR soft
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Summary An analytically representat
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Figure 3. The Irganox 1010 peak are
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The calibration curve and equation
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Select ‘Peak Ratio’ - from the
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Summary An analytically representat
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Figure 3. The GMS peak height absor
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Advanced Atomic Force Microscopy: E
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signal) image indicating the overwh
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A dependence of surface potential o
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A B C Figure 6A-C. The topography (
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A B Figure 10A-C. The topography (A
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KFM with AM-FM operation in the int
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ibbons is only slightly different f
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References 1. G. Binnig, C.F. Quate
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Figure 1. AFM topographic image of
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100nm 100nm 350nm 100nm Figure 3. A
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to perfect hexagonal patterns, whic
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Single-pass KFM studies have been k
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images due to the difference of the
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0.5 V) of the nanowires. Additional
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appeared in the topography image. T
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of PSS component. TEM studies of mo
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Atomic Force Microscopy Studies in
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on graphite and MoS2 are shown in F
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A B C D Scan size: 5 µm. Scan size
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images of PEDOT:PSS blend, (PEDOT -
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Young’s Modulus of Dielectric ‘
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Figure 3. Young’s modulus as a fu
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Nanoindentation, Scratch, and Eleva
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The samples listed as “D” sampl
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Figure 6. Elastic modulus results f
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Figure 11. Elastic modulus results
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Sample B Figure 16. Scratch curves
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Conclusions Nanoindentation and scr
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measurement technique has been expl
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References 1. J.L. Hay, “Using In
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Theory The complex shear modulus (G
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References 1. Jain, S.K., Bhattacha
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LCCC relies on carrying out isocrat
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log Mp 5 4.6 4.2 3.8 0.5 15% Water
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Authors Wei Luan and Chunxiao Wang
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Table 1. Chemical Information of An
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Injection Volume Influence of Real
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translator into three modes on diff
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To identify the matrix influence on
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Authors Chun-Xiao Wang and Wei Luan
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Table 1. Polymer Additives in ASTM
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1 2 3 4 5 1 Tinuvin P 2 BHT 3 BHEB
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1 Tinuvin P 2 BHT 3 BHEB 4 Isonox 1
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Authors Edgar Naegele Agilent Techn
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Results and discussion To meet the
Page 159 and 160: Authors Melissa Dunkle, Gerd Vanhoe
Page 161 and 162: Experimental The analyses were perf
Page 163 and 164: Repeatability of the SFC/MS analysi
Page 165 and 166: Conclusion The combination of the A
Page 167 and 168: Introduction Phthalates form a grou
Page 169 and 170: The analytical method was applied t
Page 171 and 172: Introduction Bisphenol A (BPA) is a
Page 173 and 174: Preparation of standards BPA and BP
Page 175 and 176: Limit of Detection (LOD) and Limit
Page 177 and 178: Sample analysis The content of BPA
Page 179 and 180: The calibration for BPA and BPF, wh
Page 181 and 182: Author Stephen Ball Agilent Technol
Page 183 and 184: Results and discussion By operating
Page 185 and 186: Introduction The wavelength of UV r
Page 187 and 188: LC parameters Premixed solutions of
Page 189 and 190: Linearity A linearity study was per
Page 191 and 192: References 1. Dr. James H. Gibson,
Page 193 and 194: Instrumentation Columns: 2 x PLgel
Page 195 and 196: Authors Greg Saunders and Ben MacCr
Page 197 and 198: Authors Greg Saunders, Ben MacCreat
Page 205 and 206: Author Greg Saunders Agilent Techno
Page 207 and 208: Figure 5. Universal calibration cur
Page 209: Methods and Materials Conditions Co
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