Application Compendium - Agilent Technologies

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observed in sample 5 at 1.71 min. Results show that avobenzone and octocrylene are the most widely used UV filter components in the sunscreen personal care products tested. From the extracted sample chromatogram, it is clear that samples b and d provide a broad range of protection against UVA, UVB and UVC rays. Sunscreen products with higher SPF values may contain higher amounts of sunscreen components and consequently chances are high that these products contain significant amounts of impurities. The Agilent 1290 Infinity LC provides an overall picture of impurity profiles in personal care products in the shortest amount of time, as demonstrated for sample e in Figure 7. Conclusions This Application Note demonstrates the baseline separation of eight FDA/EP approved sunscreen compounds in 99 seconds using the Agilent 1290 Infinity LC and the Agilent Poroshell 120 EC- C18 column. The minimum observed resolution value in the standard mix chromatogram was > 1.8. S/N values for each component at 0.25 ng level (on-column concentration) were demonstrated. Linearity was demonstrated from 0.25 ng to 200 ng on-column for all compounds. The poorest R 2 value is 0.9997 (nine levels and five replicates). Across the linearity levels, the highest observed RT standard deviation value was 0.0018 and the highest observed RT RSD value was 0.32% (n=5). This method can be effectively used to chromatograph UV filters and impurities present in sunscreen and personal care cosmetic products. mAU 300 200 100 0 400 200 0 200 100 0 200 1 DAD, Sig=304nm 3 100 0 5 7 200 100 0 400 300 200 100 0 0 0.5 1 1.5 8 6 1 7 7 5 7 5 7 unknown 8 b 6 Figure 6 Overlay of chromatogram for all six extracted samples. mAU 10 8 6 4 2 0 mAU 1600 1200 800 400 0 DAD, Sig=304nm 0.5 1 1.5 5 0 0.5 1 1.5 1.472 a c d e f min CompoundName Abs max 1 Aminobenzoic acid 288 2 Dioxybenzone 284 3 Oxybenzone 288 4 4-Methyl benzylidene camphor 300 5 Avobenzone 358 6 Octyl methoxycinnamate 308 7 Octocrylene 304 8 Octyl salicylate 304 Figure 7 Full scale and zoomed chromatogram of sample “e”, which demonstrates well resolved impurity peaks. These minor unidentified peaks may be parabens or other listed ingredients of the formulations and related impurities. min 1.472 min
References 1. Dr. James H. Gibson, Senior Research Scientist Director, USDA UVB Monitoring Program Natural Resource Ecology Laboratory Colorado State University. 2. Jesus Mercado-Blanco, Fernando Garcia, Manuel Fernandez-Lopez and Jose Olivares, Melanin Production by Rhizobium meliloti GR4 Is Linked to Nonsymbiotic Plasmid pRmeGR4b: Cloning, Sequencing and Expression of the Tyrosinase Gene mepA, Journal of Bacteriology, Sept. 1993, p. 5403-5410. 3. David A. Katz, Sunscreens: Preparation and evaluation 2003. 4. Ken Klein, Cosmetech Laboratories Inc: Emulsion and sunscreen, (pub date: 2001). 5. Marc.S.Reisch C&EN northeast news bureau, Chemical and Engineering news, April 11 2005, Volume 83 Number 15, pp 18-22. 6. Hanson Kerry M.; Gratton Enrico; Bardeen Christopher J. (2006). "Sunscreen enhancement of UVinduced reactive oxygen species in the skin," Free Radical Biology and Medicine 41 (8): 1205–1212. www.agilent.com/chem/lc © Agilent Technologies, Inc., 2010 December 1, 2010 Publication Number 5990-6861EN
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MATERIALS TESTING AND RESEARCH SOLU
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When accuracy and reliability in me
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AGILENT TECHNOLOGIES APPLICATIONS F
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Figure 1. Agilent Cary 630 FTIR spe
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The calibration samples were measur
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Author Frank Higgins Agilent Techno
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Figure 1. The overlaid aliphatic be
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Author John Seelenbinder Agilent Te
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Figure 3. Sample is lightly pressed
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Author Dr. Mustafa Kansiz Agilent T
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microscopy provides for a factor of
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Standard ATR IR Image No Pressure (
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A visual inspection of the sample u
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Experimental Instrumentation To per
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Authors Jonah Kirkwood † and Must
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Spectra were collected from each lo
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Infrared mapping allows for multipl
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The spectra in Figure 2 are visuall
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Conclusion Agilent‟s Cary 610 FTI
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Authors Dr. Wayne Collins*, John Se
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The calibration curve obtained for
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Conclusion Select ‘Peak Ratio’
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Summary This method describes a pro
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Method configuration To determine t
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The calibration curve for the deter
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Figure 6. The MicroLab PC FTIR soft
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Summary An analytically representat
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Figure 3. The Irganox 1010 peak are
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The calibration curve and equation
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Select ‘Peak Ratio’ - from the
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Summary An analytically representat
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Figure 3. The GMS peak height absor
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Advanced Atomic Force Microscopy: E
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signal) image indicating the overwh
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A dependence of surface potential o
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A B C Figure 6A-C. The topography (
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A B Figure 10A-C. The topography (A
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KFM with AM-FM operation in the int
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ibbons is only slightly different f
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References 1. G. Binnig, C.F. Quate
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Figure 1. AFM topographic image of
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100nm 100nm 350nm 100nm Figure 3. A
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to perfect hexagonal patterns, whic
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Single-pass KFM studies have been k
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images due to the difference of the
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0.5 V) of the nanowires. Additional
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appeared in the topography image. T
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of PSS component. TEM studies of mo
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Atomic Force Microscopy Studies in
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on graphite and MoS2 are shown in F
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A B C D Scan size: 5 µm. Scan size
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images of PEDOT:PSS blend, (PEDOT -
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Young’s Modulus of Dielectric ‘
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Figure 3. Young’s modulus as a fu
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Nanoindentation, Scratch, and Eleva
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The samples listed as “D” sampl
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Figure 6. Elastic modulus results f
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Figure 11. Elastic modulus results
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Sample B Figure 16. Scratch curves
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Conclusions Nanoindentation and scr
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measurement technique has been expl
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References 1. J.L. Hay, “Using In
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Theory The complex shear modulus (G
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References 1. Jain, S.K., Bhattacha
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LCCC relies on carrying out isocrat
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log Mp 5 4.6 4.2 3.8 0.5 15% Water
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Authors Wei Luan and Chunxiao Wang
Page 139 and 140: Table 1. Chemical Information of An
Page 141 and 142: Injection Volume Influence of Real
Page 143 and 144: translator into three modes on diff
Page 145 and 146: To identify the matrix influence on
Page 147 and 148: Authors Chun-Xiao Wang and Wei Luan
Page 149 and 150: Table 1. Polymer Additives in ASTM
Page 151 and 152: 1 2 3 4 5 1 Tinuvin P 2 BHT 3 BHEB
Page 153 and 154: 1 Tinuvin P 2 BHT 3 BHEB 4 Isonox 1
Page 155 and 156: Authors Edgar Naegele Agilent Techn
Page 157 and 158: Results and discussion To meet the
Page 159 and 160: Authors Melissa Dunkle, Gerd Vanhoe
Page 161 and 162: Experimental The analyses were perf
Page 163 and 164: Repeatability of the SFC/MS analysi
Page 165 and 166: Conclusion The combination of the A
Page 167 and 168: Introduction Phthalates form a grou
Page 169 and 170: The analytical method was applied t
Page 171 and 172: Introduction Bisphenol A (BPA) is a
Page 173 and 174: Preparation of standards BPA and BP
Page 175 and 176: Limit of Detection (LOD) and Limit
Page 177 and 178: Sample analysis The content of BPA
Page 179 and 180: The calibration for BPA and BPF, wh
Page 181 and 182: Author Stephen Ball Agilent Technol
Page 183 and 184: Results and discussion By operating
Page 185 and 186: Introduction The wavelength of UV r
Page 187 and 188: LC parameters Premixed solutions of
Page 189: Linearity A linearity study was per
Page 193 and 194: Instrumentation Columns: 2 x PLgel
Page 195 and 196: Authors Greg Saunders and Ben MacCr
Page 197 and 198: Authors Greg Saunders, Ben MacCreat
Page 205 and 206: Author Greg Saunders Agilent Techno
Page 207 and 208: Figure 5. Universal calibration cur
Page 209 and 210: Methods and Materials Conditions Co
Page 211 and 212: Materials and Methods A column set
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