Application Compendium - Agilent Technologies

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Author Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India Agilent Application Solution Analysis of bisphenol A leaching from baby feeding bottles Application Note Consumer Products Abstract Bisphenol A can leach out from the plastic surfaces of food containers and has been detected in various matrices such as plasma, urine and groundwater. Bipshenol A is an endocrine disruptor, which can mimic the body’s own hormones such as estrogen and may lead to negative health effects. In this Application Note, we describe a method to quantify bisphenol A and a structurally similar analog bisphenol F extracted from baby feeding bottles. The method was developed on an Agilent 1260 Infi nity LC system using an Agilent ZORBAX Eclipse Plus C18 column. Partial method validation was performed to demonstrate linearity, robustness and precision of area and retention time. The dilution series was generated automatically using an Agilent 7696A Sample Prep WorkBench, saving analyst time. The limit of quantitation (LOQ) for bisphenol A was found to be 1.06 ng/mL. During sample recovery studies, 80% recovery was obtained for bisphenol A. The method was transferred to an ultrahigh performance liquid chromatography (UHPLC) method using an Agilent 1290 Infi nity LC System. The UHPLC method has the same experimental conditions but showed narrower and higher peaks, better resolution, and improved signal-to-noise response. Both methods can be applied for bisphenol A quantifi cation in quality control of food containers such as baby feeding bottles.
Introduction Bisphenol A (BPA) is a monomer used to make polycarbonate plastic and epoxy resins. Traces of BPA can leach out of these polycarbonate plastic surfaces under various environmental conditions such as heat or pH changes and eventually are consumed by humans. BPA was detected in various matrices such as urine, groundwater and plasma. Based on the assumption that a threshold exists above which toxic effects are seen, the U.S. Environmental Protection Agency (US EPA) has established 50 µg/kg body weight/day as the reference dose (RfD) for BPA 1 . Baby bottles made of polycarbonate plastic are a potential risk to children. In this Application Note, we used a structurally similar commercially available compound bisphenol F, (BPF), (Figure 1) along with BPA to determine separation effi ciency. Ballesteros-Gomez et.al., reviewed various analytical methods used to separate, identify and quantify BPA 2 . Also, the ASTM standard test method, D 7574-09, describes a SPE based offl ine method for extracting bisphenol A from environmental waters 3 . BPA is a fl uorescent compound, and fl uorescence detector (FLD) is sensitive to detect BPA concentration in baby bottles. In this Application Note, a method is described to simultaneously quantify BPA and BPF using a SPE based offl ine extraction procedure with (U)HPLC/FLD detection. Experimental Instruments and software An Agilent 1260 Infi nity Binary LC system consisting of the following modules was used: Agilent 1260 Infi nity Binary Pump (G1312B) Agilent 1260 Infi nity Autosampler and Thermostat (G1367E, G1330B) Agilent 1260 Infi nity Thermostatted Column Compartment (TCC) (G1316A) Agilent 1260 Infi nity Fluorescence Detector (G1312B) with 8 µL fl ow cell The UHPLC analysis was developed and performed using an Agilent 1290 Infi nity LC System consisting of the following modules: Agilent 1290 Infi nity Binary Pump (G4220A) Agilent 1290 Infi nity Autosampler and Thermostat (G4226A, G1330B) Agilent 1290 Infi nity Thermostatted Column Compartment (G1316C) Agilent 1260 Infi nity Fluorescence Detector (G1312B) with 8 µL fl ow cell Software: Agilent ChemStation B.04.02 Sample preparation: Agilent 7696A Sample Prep WorkBench 2 HO HO CH 3 CH 3 Bisphenol A Bisphenol F OH OH Figure 1 Molecular structures of bisphenol A and bisphenol F
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MATERIALS TESTING AND RESEARCH SOLU
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When accuracy and reliability in me
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AGILENT TECHNOLOGIES APPLICATIONS F
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Figure 1. Agilent Cary 630 FTIR spe
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The calibration samples were measur
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Author Frank Higgins Agilent Techno
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Figure 1. The overlaid aliphatic be
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Author John Seelenbinder Agilent Te
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Figure 3. Sample is lightly pressed
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Author Dr. Mustafa Kansiz Agilent T
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microscopy provides for a factor of
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Standard ATR IR Image No Pressure (
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A visual inspection of the sample u
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Experimental Instrumentation To per
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Authors Jonah Kirkwood † and Must
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Spectra were collected from each lo
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Infrared mapping allows for multipl
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The spectra in Figure 2 are visuall
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Conclusion Agilent‟s Cary 610 FTI
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Authors Dr. Wayne Collins*, John Se
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The calibration curve obtained for
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Conclusion Select ‘Peak Ratio’
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Summary This method describes a pro
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Method configuration To determine t
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The calibration curve for the deter
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Figure 6. The MicroLab PC FTIR soft
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Summary An analytically representat
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Figure 3. The Irganox 1010 peak are
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The calibration curve and equation
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Select ‘Peak Ratio’ - from the
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Summary An analytically representat
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Figure 3. The GMS peak height absor
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Advanced Atomic Force Microscopy: E
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signal) image indicating the overwh
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A dependence of surface potential o
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A B C Figure 6A-C. The topography (
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A B Figure 10A-C. The topography (A
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KFM with AM-FM operation in the int
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ibbons is only slightly different f
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References 1. G. Binnig, C.F. Quate
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Figure 1. AFM topographic image of
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100nm 100nm 350nm 100nm Figure 3. A
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to perfect hexagonal patterns, whic
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Single-pass KFM studies have been k
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images due to the difference of the
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0.5 V) of the nanowires. Additional
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appeared in the topography image. T
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of PSS component. TEM studies of mo
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Atomic Force Microscopy Studies in
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on graphite and MoS2 are shown in F
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A B C D Scan size: 5 µm. Scan size
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images of PEDOT:PSS blend, (PEDOT -
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Young’s Modulus of Dielectric ‘
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Figure 3. Young’s modulus as a fu
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Nanoindentation, Scratch, and Eleva
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The samples listed as “D” sampl
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Figure 6. Elastic modulus results f
Page 119 and 120: Figure 11. Elastic modulus results
Page 121 and 122: Sample B Figure 16. Scratch curves
Page 123 and 124: Conclusions Nanoindentation and scr
Page 125 and 126: measurement technique has been expl
Page 127 and 128: References 1. J.L. Hay, “Using In
Page 129 and 130: Theory The complex shear modulus (G
Page 131 and 132: References 1. Jain, S.K., Bhattacha
Page 133 and 134: LCCC relies on carrying out isocrat
Page 135 and 136: log Mp 5 4.6 4.2 3.8 0.5 15% Water
Page 137 and 138: Authors Wei Luan and Chunxiao Wang
Page 139 and 140: Table 1. Chemical Information of An
Page 141 and 142: Injection Volume Influence of Real
Page 143 and 144: translator into three modes on diff
Page 145 and 146: To identify the matrix influence on
Page 147 and 148: Authors Chun-Xiao Wang and Wei Luan
Page 149 and 150: Table 1. Polymer Additives in ASTM
Page 151 and 152: 1 2 3 4 5 1 Tinuvin P 2 BHT 3 BHEB
Page 153 and 154: 1 Tinuvin P 2 BHT 3 BHEB 4 Isonox 1
Page 155 and 156: Authors Edgar Naegele Agilent Techn
Page 157 and 158: Results and discussion To meet the
Page 159 and 160: Authors Melissa Dunkle, Gerd Vanhoe
Page 161 and 162: Experimental The analyses were perf
Page 163 and 164: Repeatability of the SFC/MS analysi
Page 165 and 166: Conclusion The combination of the A
Page 167 and 168: Introduction Phthalates form a grou
Page 169: The analytical method was applied t
Page 173 and 174: Preparation of standards BPA and BP
Page 175 and 176: Limit of Detection (LOD) and Limit
Page 177 and 178: Sample analysis The content of BPA
Page 179 and 180: The calibration for BPA and BPF, wh
Page 181 and 182: Author Stephen Ball Agilent Technol
Page 183 and 184: Results and discussion By operating
Page 185 and 186: Introduction The wavelength of UV r
Page 187 and 188: LC parameters Premixed solutions of
Page 189 and 190: Linearity A linearity study was per
Page 191 and 192: References 1. Dr. James H. Gibson,
Page 193 and 194: Instrumentation Columns: 2 x PLgel
Page 195 and 196: Authors Greg Saunders and Ben MacCr
Page 197 and 198: Authors Greg Saunders, Ben MacCreat
Page 205 and 206: Author Greg Saunders Agilent Techno
Page 207 and 208: Figure 5. Universal calibration cur
Page 209 and 210: Methods and Materials Conditions Co
Page 211 and 212: Materials and Methods A column set
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