Application Compendium - Agilent Technologies

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To test the method, a blank matrix sample produced from a heavily colored napkin was spiked with the six PAAs at various concentrations (Figure 3). All six PAAs could be well separated from the matrix compounds and identifi ed and quantifi ed with the developed method and calibration. However, for some PAAs, a sample cleaning step using SPE prior to the chromatographic analysis might be necessary as indicated by a recovery that was too high, for example, of o-toluidine. This may have been caused by matrix interferences and has to be considered in further method development activities. Conclusion This Application Note shows that the Agilent 1290 Infi nity LC System can detect low amounts of primary aromatic amines by using the 60-mm Max-Light high sensitivity cell in the diode array detector, and the 1290 large volume injection kit in the autosampler for an enrichment step. Data are shown which prove an excellent linearity of the calibration down to the LOQ at 2 ppb. The LOD was found at 0.5 ppb. The lower level calibration points show good relative standard deviations for the retention time and the area as well as good precision for the measured concentrations. The PAAs could be detected and quantifi ed in a sample with heavy matrix load at lowest levels. Reference 1. Enhancing the maximum injection volume of the Agilent 1290 Infi nity Autosampler, Agilent Technical Overview, Publication Number 5990-9533EN, 2011. Compound Aniline o-Anisidine o-Toluidine Compound Compound Compound response response response RT Area Amount factor RT Area Amount factor RT Area Amount factor Average 3.273 22.474 9.610 0.427 4.815 19.131 9.988 0.522 5.084 20.848 10.014 0.480 Standard deviation 0.001 0.117 0.048 7.049E-05 0.002 0.094 0.048 2.870E-05 0.002 0.567 0.271 5.390E-05 RSD (%) 0.039 0.521 0.505 0.016 0.047 0.494 0.489 0.005 0.047 2.722 2.715 0.011 Compound 2-Methyl-5-nitroaniline 2,4-Dimethylaniline 2,4-Dichloraniline Compound Compound Compound response response response RT Area Amount factor RT Area Amount factor RT Area Amount factor Average 6.400 31.729 9.913 0.312 7.827 22.871 9.925 0.433 11.367 28.067 10.050 0.358 Standard deviation 0.004 0.279 0.087 1.298E-05 0.004 0.198 0.086 2.950E-05 0.006 0.321 0.116 6.251E-05 RSD (%) 0.057 0.880 0.880 0.004 0.050 0.869 0.875 0.006 0.049 1.146 1.163 0.017 Table 1 Retention time precision, area precision, concentration precision and compound response factor precision for the six used standard paAs at 10 ng/mL. mAU 140 120 100 80 60 40 20 0 3.258 _ Aniline 4.787 _ o-Anisidine 5.057 _ o-Toluidine 6.365 _ 2,Methyl-5-nitroaniline 2 4 6 8 10 12 14 16 Figure 3 Matrix sample from a heavily colored napkin spiked with the six paAs. 7.801 _ 2,4-Dimethylaniline 11.422 _ 2,4-Dichloraniline www.agilent.com/chem/1200 © Agilent Technologies, Inc., 2012 Published in the USA, February 10, 2012 5990-9751EN min
Authors Melissa Dunkle, Gerd Vanhoenacker, Frank David, Pat Sandra Research Institute for Chromatography, President Kennedypark 26, 8500 Kortrijk, Belgium Martin Vollmer Agilent Technologies Inc. Waldbronn, Germany Determination of polymer additives and migration products prevalent in food packaging material Using the Agilent 1260 Infi nity SFC System with the Agilent 6130 Single Quadrupole LC/MS System Application Note Consumer Product, Food Safety 600000 400000 200000 0 120000 80000 40000 0 300000 200000 100000 0 A) TIC Abstract DiDP T234 I1010 ERU, T440 I168 1 2 3 4 5 6 7 8 9 B) EIC 338 ERU 1 2 3 4 5 6 7 8 9 C) EIC 436 T440 1 2 3 4 5 6 7 8 9 According to EU directive 2002/72, materials that come into contact with food products should be tested for possible leachables. Supercritical fl uid chromatography (SFC) is a complimentary separation technique to HPLC for the analysis and potential detection of polymer additives that might be released out of packaging material. This Application Note demonstrates the applicability of SFC for typical polymer additives using the Agilent 1260 Infi nity Analytical SFC System in combination with an Agilent 6130 Single Quadrupole LC/MS System. min min min
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MATERIALS TESTING AND RESEARCH SOLU
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When accuracy and reliability in me
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AGILENT TECHNOLOGIES APPLICATIONS F
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Figure 1. Agilent Cary 630 FTIR spe
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The calibration samples were measur
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Author Frank Higgins Agilent Techno
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Figure 1. The overlaid aliphatic be
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Author John Seelenbinder Agilent Te
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Figure 3. Sample is lightly pressed
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Author Dr. Mustafa Kansiz Agilent T
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microscopy provides for a factor of
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Standard ATR IR Image No Pressure (
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A visual inspection of the sample u
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Experimental Instrumentation To per
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Authors Jonah Kirkwood † and Must
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Spectra were collected from each lo
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Infrared mapping allows for multipl
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The spectra in Figure 2 are visuall
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Conclusion Agilent‟s Cary 610 FTI
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Authors Dr. Wayne Collins*, John Se
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The calibration curve obtained for
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Conclusion Select ‘Peak Ratio’
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Summary This method describes a pro
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Method configuration To determine t
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The calibration curve for the deter
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Figure 6. The MicroLab PC FTIR soft
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Summary An analytically representat
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Figure 3. The Irganox 1010 peak are
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The calibration curve and equation
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Select ‘Peak Ratio’ - from the
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Summary An analytically representat
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Figure 3. The GMS peak height absor
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Advanced Atomic Force Microscopy: E
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signal) image indicating the overwh
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A dependence of surface potential o
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A B C Figure 6A-C. The topography (
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A B Figure 10A-C. The topography (A
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KFM with AM-FM operation in the int
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ibbons is only slightly different f
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References 1. G. Binnig, C.F. Quate
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Figure 1. AFM topographic image of
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100nm 100nm 350nm 100nm Figure 3. A
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to perfect hexagonal patterns, whic
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Single-pass KFM studies have been k
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images due to the difference of the
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0.5 V) of the nanowires. Additional
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appeared in the topography image. T
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of PSS component. TEM studies of mo
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Atomic Force Microscopy Studies in
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on graphite and MoS2 are shown in F
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A B C D Scan size: 5 µm. Scan size
Page 107 and 108: images of PEDOT:PSS blend, (PEDOT -
Page 109 and 110: Young’s Modulus of Dielectric ‘
Page 111 and 112: Figure 3. Young’s modulus as a fu
Page 113 and 114: Nanoindentation, Scratch, and Eleva
Page 115 and 116: The samples listed as “D” sampl
Page 117 and 118: Figure 6. Elastic modulus results f
Page 119 and 120: Figure 11. Elastic modulus results
Page 121 and 122: Sample B Figure 16. Scratch curves
Page 123 and 124: Conclusions Nanoindentation and scr
Page 125 and 126: measurement technique has been expl
Page 127 and 128: References 1. J.L. Hay, “Using In
Page 129 and 130: Theory The complex shear modulus (G
Page 131 and 132: References 1. Jain, S.K., Bhattacha
Page 133 and 134: LCCC relies on carrying out isocrat
Page 135 and 136: log Mp 5 4.6 4.2 3.8 0.5 15% Water
Page 137 and 138: Authors Wei Luan and Chunxiao Wang
Page 139 and 140: Table 1. Chemical Information of An
Page 141 and 142: Injection Volume Influence of Real
Page 143 and 144: translator into three modes on diff
Page 145 and 146: To identify the matrix influence on
Page 147 and 148: Authors Chun-Xiao Wang and Wei Luan
Page 149 and 150: Table 1. Polymer Additives in ASTM
Page 151 and 152: 1 2 3 4 5 1 Tinuvin P 2 BHT 3 BHEB
Page 153 and 154: 1 Tinuvin P 2 BHT 3 BHEB 4 Isonox 1
Page 155 and 156: Authors Edgar Naegele Agilent Techn
Page 157: Results and discussion To meet the
Page 161 and 162: Experimental The analyses were perf
Page 163 and 164: Repeatability of the SFC/MS analysi
Page 165 and 166: Conclusion The combination of the A
Page 167 and 168: Introduction Phthalates form a grou
Page 169 and 170: The analytical method was applied t
Page 171 and 172: Introduction Bisphenol A (BPA) is a
Page 173 and 174: Preparation of standards BPA and BP
Page 175 and 176: Limit of Detection (LOD) and Limit
Page 177 and 178: Sample analysis The content of BPA
Page 179 and 180: The calibration for BPA and BPF, wh
Page 181 and 182: Author Stephen Ball Agilent Technol
Page 183 and 184: Results and discussion By operating
Page 185 and 186: Introduction The wavelength of UV r
Page 187 and 188: LC parameters Premixed solutions of
Page 189 and 190: Linearity A linearity study was per
Page 191 and 192: References 1. Dr. James H. Gibson,
Page 193 and 194: Instrumentation Columns: 2 x PLgel
Page 195 and 196: Authors Greg Saunders and Ben MacCr
Page 197 and 198: Authors Greg Saunders, Ben MacCreat
Page 205 and 206: Author Greg Saunders Agilent Techno
Page 207 and 208: Figure 5. Universal calibration cur
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Methods and Materials Conditions Co
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Materials and Methods A column set
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Authors Greg Saunders, Ben MacCreat
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Authors Greg Saunders, Ben MacCreat
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