Application Compendium - Agilent Technologies

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Optimized Column Temperature Increasing column temperature can lower both solvent viscosity and nonspecific column/analyte interactions. The new ZORBAX StableBond RRHT columns can operate at temperatures up to 90 °C. We tested operating temperatures at 60, 75, 85, and 90 °C with a ZORBAX SB-C8 4.6 mm × 150 mm, 5-µm column. The results (Figure 5) show that the analysis time obtained from 60 °C to 85 °C is reduced from 23.5 minutes to 17 minutes; at 90 °C, only an additional 0.5 minute is saved. Based on the combined speed reduction and optimized resolution of peaks 4 and 5, 85 °C is chosen as a suitable column temperature. 6 T = 60 °C F = 1 mL/min 2.5 5 7.5 10 12.5 15 17.5 20 22.5 T = 75 °C F = 1 mL/min T = 85 °C F = 1 mL/min T = 90 °C F = 1 mL/min 1 1 2 3 4 2 4 6 8 10 12 14 16 18 1 2 4 6 8 10 12 14 16 min 1 The method was then scaled in flow and time for exact translation to a 4.6 mm × 50 mm, 1.8-µm column (Figure 6). Finally, the separation was optimized for faster separation by increasing the flow rate from 1 mL/min to 3.5 mL/min, with only a 1.7-minute analysis time (Figure 7). This is really an excellent procedure for high-throughput screening and quantitation of a large number of samples. Figure 8, the separation of an extract of linear lowdensity polyethylene (LLDPE) spiked with 20 µg/mL of standard solution, shows excellent separation with real sample matrix. 2 4 6 8 10 12 14 16 min 5 2 3 4 2 2 3 5 6 4 5 3 4 5 6 1 Tinuvin P 2 BHT 3 BHEB 4 Isonox 129 5 Ernucamide-E 6 Irganox 1010 7 Irganox 1076 Figure 5. Separation of additives standards on ZORBAX StableBond RRHT SB-C18, 4.6 mm × 150 mm, 1.8 µm. 6 6 7 7 min min 7 7
1 Tinuvin P 2 BHT 3 BHEB 4 Isonox 129 5 Ernucamide-E 6 Irganox 1010 7 Irganox 1076 1 1 2 3 4 5 min Sample: Standard 200 mg/mL Sample size: 2 µL Detector: UV 200 nm Mobile phase: A: water B: acetonitrile Gradient slope: 6.8% Flow rate: 1mL/min Figure 6. Separation of additives standards on ZORBAX SB-C18, 4.6 mm × 50 mm, 1.8 µm, at 85 °C. 1 Tinuvin P 2 BHT 3 BHEB 4 Isonox 129 5 Ernucamide-E 6 Irganox 1010 7 Irganox 1076 1 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 Sample: Standard_200 mg/mL Sample size: 2 µL Detector: UV 200 nm Mobile phase: A: water B: acetonitrile Gradient slope: 6.8% Flow rate: 3.5 mL/min 0 2 Figure 7. Fast separation of additives standards on ZORBAX SB-C18, 4.6 mm × 50 mm, 1.8 µm, at 85 °C. mAU 2 100 3 80 1 Tinuvin P 2 BHT 4 60 3 BHEB 4 Isonox 129 1 6 7 40 5 Ernucamide-E 6 Irganox 1010 20 7 Irganox 1076 5 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 LC conditions are identical with those in Figure 7. Figure 8. Fast separation of spiked real sample-LLDPE (20 µg/mL) on ZORBAX SB-C18, 4.6 mm × 50 mm, 1.8 µm, at 85 °C. 2 3 3 4 5 4 5 6 6 7 7 min min 7
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MATERIALS TESTING AND RESEARCH SOLU
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When accuracy and reliability in me
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AGILENT TECHNOLOGIES APPLICATIONS F
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Figure 1. Agilent Cary 630 FTIR spe
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The calibration samples were measur
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Author Frank Higgins Agilent Techno
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Figure 1. The overlaid aliphatic be
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Author John Seelenbinder Agilent Te
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Figure 3. Sample is lightly pressed
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Author Dr. Mustafa Kansiz Agilent T
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microscopy provides for a factor of
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Standard ATR IR Image No Pressure (
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A visual inspection of the sample u
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Experimental Instrumentation To per
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Authors Jonah Kirkwood † and Must
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Spectra were collected from each lo
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Infrared mapping allows for multipl
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The spectra in Figure 2 are visuall
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Conclusion Agilent‟s Cary 610 FTI
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Authors Dr. Wayne Collins*, John Se
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The calibration curve obtained for
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Conclusion Select ‘Peak Ratio’
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Summary This method describes a pro
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Method configuration To determine t
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The calibration curve for the deter
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Figure 6. The MicroLab PC FTIR soft
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Summary An analytically representat
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Figure 3. The Irganox 1010 peak are
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The calibration curve and equation
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Select ‘Peak Ratio’ - from the
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Summary An analytically representat
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Figure 3. The GMS peak height absor
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Advanced Atomic Force Microscopy: E
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signal) image indicating the overwh
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A dependence of surface potential o
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A B C Figure 6A-C. The topography (
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A B Figure 10A-C. The topography (A
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KFM with AM-FM operation in the int
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ibbons is only slightly different f
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References 1. G. Binnig, C.F. Quate
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Figure 1. AFM topographic image of
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100nm 100nm 350nm 100nm Figure 3. A
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to perfect hexagonal patterns, whic
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Single-pass KFM studies have been k
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images due to the difference of the
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0.5 V) of the nanowires. Additional
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appeared in the topography image. T
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of PSS component. TEM studies of mo
Page 101 and 102: Atomic Force Microscopy Studies in
Page 103 and 104: on graphite and MoS2 are shown in F
Page 105 and 106: A B C D Scan size: 5 µm. Scan size
Page 107 and 108: images of PEDOT:PSS blend, (PEDOT -
Page 109 and 110: Young’s Modulus of Dielectric ‘
Page 111 and 112: Figure 3. Young’s modulus as a fu
Page 113 and 114: Nanoindentation, Scratch, and Eleva
Page 115 and 116: The samples listed as “D” sampl
Page 117 and 118: Figure 6. Elastic modulus results f
Page 119 and 120: Figure 11. Elastic modulus results
Page 121 and 122: Sample B Figure 16. Scratch curves
Page 123 and 124: Conclusions Nanoindentation and scr
Page 125 and 126: measurement technique has been expl
Page 127 and 128: References 1. J.L. Hay, “Using In
Page 129 and 130: Theory The complex shear modulus (G
Page 131 and 132: References 1. Jain, S.K., Bhattacha
Page 133 and 134: LCCC relies on carrying out isocrat
Page 135 and 136: log Mp 5 4.6 4.2 3.8 0.5 15% Water
Page 137 and 138: Authors Wei Luan and Chunxiao Wang
Page 139 and 140: Table 1. Chemical Information of An
Page 141 and 142: Injection Volume Influence of Real
Page 143 and 144: translator into three modes on diff
Page 145 and 146: To identify the matrix influence on
Page 147 and 148: Authors Chun-Xiao Wang and Wei Luan
Page 149 and 150: Table 1. Polymer Additives in ASTM
Page 151: 1 2 3 4 5 1 Tinuvin P 2 BHT 3 BHEB
Page 155 and 156: Authors Edgar Naegele Agilent Techn
Page 157 and 158: Results and discussion To meet the
Page 159 and 160: Authors Melissa Dunkle, Gerd Vanhoe
Page 161 and 162: Experimental The analyses were perf
Page 163 and 164: Repeatability of the SFC/MS analysi
Page 165 and 166: Conclusion The combination of the A
Page 167 and 168: Introduction Phthalates form a grou
Page 169 and 170: The analytical method was applied t
Page 171 and 172: Introduction Bisphenol A (BPA) is a
Page 173 and 174: Preparation of standards BPA and BP
Page 175 and 176: Limit of Detection (LOD) and Limit
Page 177 and 178: Sample analysis The content of BPA
Page 179 and 180: The calibration for BPA and BPF, wh
Page 181 and 182: Author Stephen Ball Agilent Technol
Page 183 and 184: Results and discussion By operating
Page 185 and 186: Introduction The wavelength of UV r
Page 187 and 188: LC parameters Premixed solutions of
Page 189 and 190: Linearity A linearity study was per
Page 191 and 192: References 1. Dr. James H. Gibson,
Page 193 and 194: Instrumentation Columns: 2 x PLgel
Page 195 and 196: Authors Greg Saunders and Ben MacCr
Page 197 and 198: Authors Greg Saunders, Ben MacCreat
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Authors Greg Saunders, Ben MacCreat
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Author Greg Saunders Agilent Techno
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Figure 5. Universal calibration cur
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Methods and Materials Conditions Co
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Materials and Methods A column set
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