Application Compendium - Agilent Technologies

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2 4 6 8 10 12 min Conditions Mobile phase: A: water; B: ACN ZORBAX chemistry: Eclipse XDB-C18 Eclipse XDB-C8 Flow rate: 1.5 mL/min Gradient: Min %B Min %B Wavelength: 200 nm 0.00 75 0.00 75 Injection volume: 10 µL 5.00 100 8.00 100 Column temperature: 50 ºC 25.00 100 15.00 100 Column size: 4.6 mm × 150 mm, 5 µm 25.10 75 15.10 75 Sample: Standard mixture, 200 µg/mL in isopropanol 30.00 75 20.00 75 4 Results and Discussion Selection of Stationary Phase for the Separation of Antioxidants It is desirable during method development to select a column that will provide the optimal analyte separation and shortest analysis time. Reversed phase C18 columns are recommended by ASTM D6042-04; however, in our application we deter- mAU 800 700 600 500 400 300 200 100 0 mAU 800 700 600 500 400 300 200 100 0 1 2 3 Agilent ZORBAX Eclipse XDB-C18, 4.6 mm × 150 mm, 5 µm 4 5 2.5 5 7.5 10 12.5 15 17.5 20 22.5 1 Compound 1 Tinuvin P 2 Erucamide 3 Irganox 3114 5 Vitamin E 4 Irganox 1010 7 Irganox 1076 6 Ox Irgafos 168 8 Irgafos 168 2 Compound 1 Tinuvin P 2 Erucamide 3 Irganox 3114 4 Irganox 1010 5 Vitamin E 6 Ox Irgafos 168 7 Irganox 1076 8 Irgafos 168 3 6 5 4 mined that the retention characteristics of ZORBAX XDB-C18 columns were too strong for the specified solvents, resulting in broad peak shape and quantitation difficulties for late-eluting peaks. Compared with ZORBAX XDB-C18 columns, ZORBAX XDB-C8 columns showed better retention capability and peak shape. Therefore, we chose the ZORBAX XDB-C8 column for further method development. The different separations with ZORBAX XDB-C18 and ZORBAX XDB-C8 columns are shown in the Figure 1. 7 8 Agilent ZORBAX Eclipse XDB-C8, 4.6 mm × 150 mm, 5 µm Figure 1. ZORBAX stationary phase comparison for antioxidants. 7 6 8 min
Injection Volume Influence of Real Sample Extraction Solution on the Peak Shape According to ASTM D6042-04 [1], a solvent mixture of methylene chloride and cyclohexane (1/1 v/v) is used as the extraction solvent and, after filtration, the extracted solution is directly injected into the LC. Neither methylene chloride nor cyclohexane is miscible in the acetonitrile and water mobile phase. Peak splitting was observed when the injection volume was 10 µL. We decreased the sample size of real sample and found that the volume of 5 µL was suitable and free of solvent influence. The split and nonsplit peaks are shown in Figure 2. At the same time, the influence of injection volume was not found in the standard solution, which was dissolved in isopropanol per ASTM method guidance. mAU 25 20 15 10 0 5 -5 mAU 35 30 25 20 15 10 5 0 _ 5 Sample size: 5 µL 2.5 5 7.5 10 12.5 15 17.5 20 22.5 Sample size: 10 µL 2.5 5 7.5 10 12.5 15 17.5 20 22.5 Fast Method Developed Based on New 1200 RRLC with Method Translator Due to the appearance of sub-two-micron columns and LC systems with higher pressure capabilities, the research of ultra-fast separation is more and more popular. Therefore, it is important to quickly and easily transfer conventional methods to fast or high-resolution methods. Agilent provides the users of RRLC systems with two versions of method translators; one is a Microsoft.net version, which requires that Net-Framework 2.0 be resident on the computer, the other is a Microsoft Excel version, which requires that Excel be resident on the PC. The interface of the two translators is displayed in Figure 3. Conditions Mobile phase: A: water; B: ACN Gradient: Flow rate: 1.5 mL/min Min %B Wavelength: 200 nm 0.00 75 Injection volume: 5 or 10 µL 5.00 100 Column temperature: 50 ºC 25.00 100 Column: ZORBAX Eclipse XDB-C18 4.6 mm × 150 mm, 5 µm 25.10 75 Sample: Polypropylene extraction solution 30.00 75 Figure 2. Injection volume influence of real sample extraction solution on the peak shape. min min 5
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MATERIALS TESTING AND RESEARCH SOLU
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When accuracy and reliability in me
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AGILENT TECHNOLOGIES APPLICATIONS F
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Figure 1. Agilent Cary 630 FTIR spe
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The calibration samples were measur
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Author Frank Higgins Agilent Techno
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Figure 1. The overlaid aliphatic be
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Author John Seelenbinder Agilent Te
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Figure 3. Sample is lightly pressed
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Author Dr. Mustafa Kansiz Agilent T
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microscopy provides for a factor of
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Standard ATR IR Image No Pressure (
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A visual inspection of the sample u
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Experimental Instrumentation To per
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Authors Jonah Kirkwood † and Must
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Spectra were collected from each lo
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Infrared mapping allows for multipl
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The spectra in Figure 2 are visuall
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Conclusion Agilent‟s Cary 610 FTI
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Authors Dr. Wayne Collins*, John Se
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The calibration curve obtained for
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Conclusion Select ‘Peak Ratio’
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Summary This method describes a pro
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Method configuration To determine t
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The calibration curve for the deter
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Figure 6. The MicroLab PC FTIR soft
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Summary An analytically representat
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Figure 3. The Irganox 1010 peak are
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The calibration curve and equation
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Select ‘Peak Ratio’ - from the
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Summary An analytically representat
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Figure 3. The GMS peak height absor
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Advanced Atomic Force Microscopy: E
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signal) image indicating the overwh
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A dependence of surface potential o
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A B C Figure 6A-C. The topography (
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A B Figure 10A-C. The topography (A
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KFM with AM-FM operation in the int
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ibbons is only slightly different f
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References 1. G. Binnig, C.F. Quate
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Figure 1. AFM topographic image of
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100nm 100nm 350nm 100nm Figure 3. A
Page 89 and 90: to perfect hexagonal patterns, whic
Page 91 and 92: Single-pass KFM studies have been k
Page 93 and 94: images due to the difference of the
Page 95 and 96: 0.5 V) of the nanowires. Additional
Page 97 and 98: appeared in the topography image. T
Page 99 and 100: of PSS component. TEM studies of mo
Page 101 and 102: Atomic Force Microscopy Studies in
Page 103 and 104: on graphite and MoS2 are shown in F
Page 105 and 106: A B C D Scan size: 5 µm. Scan size
Page 107 and 108: images of PEDOT:PSS blend, (PEDOT -
Page 109 and 110: Young’s Modulus of Dielectric ‘
Page 111 and 112: Figure 3. Young’s modulus as a fu
Page 113 and 114: Nanoindentation, Scratch, and Eleva
Page 115 and 116: The samples listed as “D” sampl
Page 117 and 118: Figure 6. Elastic modulus results f
Page 119 and 120: Figure 11. Elastic modulus results
Page 121 and 122: Sample B Figure 16. Scratch curves
Page 123 and 124: Conclusions Nanoindentation and scr
Page 125 and 126: measurement technique has been expl
Page 127 and 128: References 1. J.L. Hay, “Using In
Page 129 and 130: Theory The complex shear modulus (G
Page 131 and 132: References 1. Jain, S.K., Bhattacha
Page 133 and 134: LCCC relies on carrying out isocrat
Page 135 and 136: log Mp 5 4.6 4.2 3.8 0.5 15% Water
Page 137 and 138: Authors Wei Luan and Chunxiao Wang
Page 139: Table 1. Chemical Information of An
Page 143 and 144: translator into three modes on diff
Page 145 and 146: To identify the matrix influence on
Page 147 and 148: Authors Chun-Xiao Wang and Wei Luan
Page 149 and 150: Table 1. Polymer Additives in ASTM
Page 151 and 152: 1 2 3 4 5 1 Tinuvin P 2 BHT 3 BHEB
Page 153 and 154: 1 Tinuvin P 2 BHT 3 BHEB 4 Isonox 1
Page 155 and 156: Authors Edgar Naegele Agilent Techn
Page 157 and 158: Results and discussion To meet the
Page 159 and 160: Authors Melissa Dunkle, Gerd Vanhoe
Page 161 and 162: Experimental The analyses were perf
Page 163 and 164: Repeatability of the SFC/MS analysi
Page 165 and 166: Conclusion The combination of the A
Page 167 and 168: Introduction Phthalates form a grou
Page 169 and 170: The analytical method was applied t
Page 171 and 172: Introduction Bisphenol A (BPA) is a
Page 173 and 174: Preparation of standards BPA and BP
Page 175 and 176: Limit of Detection (LOD) and Limit
Page 177 and 178: Sample analysis The content of BPA
Page 179 and 180: The calibration for BPA and BPF, wh
Page 181 and 182: Author Stephen Ball Agilent Technol
Page 183 and 184: Results and discussion By operating
Page 185 and 186: Introduction The wavelength of UV r
Page 187 and 188: LC parameters Premixed solutions of
Page 189 and 190: Linearity A linearity study was per
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References 1. Dr. James H. Gibson,
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Instrumentation Columns: 2 x PLgel
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Authors Greg Saunders and Ben MacCr
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Authors Greg Saunders, Ben MacCreat
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Author Greg Saunders Agilent Techno
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Figure 5. Universal calibration cur
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Methods and Materials Conditions Co
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Materials and Methods A column set
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