XIX Sympozjum Srodowiskowe PTZE - materialy.pdf

XIX Sympozjum PTZE, Worliny 2009
0.8 mT, 20 kHz IF-MF, respectively as typical results. All of other condition also did not
show any difference between control and exposure (Data not shown).
In in vitro micronucleus test, neither
significant nor reproducible difference
between MF exposed and unexposed
control cells was found in the
micronucleus formation rates in 2 kHz,
0.8 mT IF-MF and also 20 kHz,
0,8 mT IF-MF(Fig. 3), respectively.
These results suggest that exposure to
2 kHz, 10 kHz and 20 kHz, up to
1 mT sinusoidal IF-MFs did not
induced gene mutations, larger scale
chromosomal changes, recombination, aneuploidy and others that could be detected by
genotoxicity test systems in this study. Thus, our results suggest that sinusoidal IF-MFs (2, 10
and 20 kHz) did not have mutagenic potential even in the MF strenghth was exceeded
100 times of the reference level in general public exposure of ICNIRP guideline.
4. Conclusion
Experimental results suggest that exposure to 2, 10 and 20 kHz, up to 1 mT sinusoidal IF-MFs
did not have any potential to induce genetic damages that could be detected by Ames assay,
MLA and in vitro micronucleus test.
References
Micronucleus form ation /1000 cells)
100
90
80
70
60
50
40
30
20
10
0
[1] NAKASONO S, et al., “Intermediate frequency magnetic fields do not have mutagenic,
co-mutagenic or gene conversion potentials in microbial genotoxicity tests”, Mut. Res.
(2008) Vol. 649, 187-200.
63
*
Treatm ent
Fig. 3 Result of micronucleus formation in 20 kHz,
0.8 mT magnetic field
*
C ontrol
Exposure
C ontrol (MMC)
Exposure (M M C)
*; p