0739 v1.2 Duolink Brochure - final - Eurogentec
0739 v1.2 Duolink Brochure - final - Eurogentec
0739 v1.2 Duolink Brochure - final - Eurogentec
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
9. ACCURATELY QUANTIFY EXPRESSION LEVELS<br />
Discrete fluorescent signals reveal expression of EGF receptor in A431 cells.<br />
Primary antibody against EGFR<br />
HIGH SIGNAL TO NOISE<br />
1000<br />
100<br />
10<br />
1<br />
Immunofluorescence<br />
<strong>Duolink</strong><br />
Negative control,<br />
no primary antibody added<br />
Fig 12. PLA signal-to-noise<br />
ratio 100 times higher than<br />
immunofluorescence (data derived<br />
from comparison with controls - no<br />
primary antibody added).<br />
Fig 11. Red: EGF receptors.<br />
Blue: DAPI stained nuclei.<br />
“… a state of the art quantitative protein<br />
technology that has furthered our research on<br />
human brain tumors above and beyond classical<br />
protein techniques.“<br />
Markus Bredel, MD, PhD,<br />
Northwestern University, Chicago<br />
<strong>Duolink</strong> – Protein interactions<br />
9
Change language