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0739 v1.2 Duolink Brochure - final - Eurogentec

0739 v1.2 Duolink Brochure - final - Eurogentec

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6. DIFFERENTIATE BETWEEN SUB-POPULATIONS OF CELLS<br />

Evaluate phosphorylation levels. Discrete fluorescent signals reveal phosphorylation of PDGF<br />

receptors.<br />

Unstimulated control<br />

PLA SIGNALS (SIGNALS/CELL)<br />

50<br />

45<br />

40<br />

35<br />

30<br />

25<br />

20<br />

15<br />

10<br />

5<br />

0<br />

NON STIMULATED STIMULATED<br />

Fig 8. Dedicated <strong>Duolink</strong> ImageTool<br />

generates accurate data.<br />

BB (PDGF ligand)-stimulated cells<br />

Fig 7. Red: phosphorylated PDGF<br />

receptors.<br />

Blue: Hoechst stained nuclei.<br />

“… allows visualization of the cellular<br />

compartment (nucleus, cytoplasm, etc) in which<br />

protein-protein interactions take place.“<br />

Mark Wade, PhD,<br />

Gene Expression Laboratory (Wahl Lab),<br />

The Salk Institute, San Diego, USA<br />

<strong>Duolink</strong> – Protein interactions<br />

7

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