diamond Taq® Product description - Eurogentec

diamond Taq ® family 

Ultra pure Taq dNA POLYMERASES 

Suited for Diagnostic and Demanding Research PCR & qPCR 

Ultra-low 

DNA 

diamond Taq ® 

Hot diamond Taq ® 

HGS diamond Taq ®

See additional benefits in the description 

of each individual enzyme. 

General Benefits 

ê Ultra-low residual DNA content: 

98 %) and ultra-low 

bioburden (< 10 CFU/ml). Typically = 0 CFU/ml 

ê Exceptional reproducibility: GMP process 

and stringent QC ensuring lot-to-lot & results 

reproducibility 

ê Sensitive: Amplification of DNA templates 

even at very low concentrations 

ê Full traceability: Quality Management 

System fully compliant to ISO 13 485 Medical 

Device standards and FDA’s Quality System 

Regulations 

ê GMP compliant: Highly suited for 

Diagnostic kits, Lab Services (with ASRs) and 

demanding Research 

ê Suited for ISO 15189 accredited 

laboratories 

ê GMP manufacturing & purification 

processes: Minimize the risk of false 

positive results due to high leftover of 

residual DNA contamination (bacterial & 

fungal) 

ê Complete: PCR reaction buffer & MgCl 2 

included 

ê 

Documentation: Technical Data Sheet 

& Certificate of Analysis 

ê Customized Fill & Finish: Cost-effective 

tailored solution 

ê Batch reservation (on request) 

ê Particularly recommended for Diagnostic 

PCR & qPCR applications that require ultralow 

levels of bacterial & fungal DNA 

Smart 

Ladder 

+ control - 

control 

With 25 years experience, Eurogentec Genomics is a leading supplier of highquality 

reagents for genomic research to bench scientists around the globe. 

Eurogentec is also an experienced Contract Manufacturing Organization 

(CMO), operating GMP and ISO 13485-certified manufacturing facilities in 

Belgium, USA and Japan. We offer a wide range of oligonucleotide-based 

components for Diagnostic and Therapeutic applications. 

In addition, Eurogentec Genomics has joined forces with Eurogentec 

Biologics, a leader in GMP manufacturing of biopharmaceuticals, to offer 

a growing range of ultrapure Taq DNA Polymerases highly suited for 

Diagnostic and demanding Research PCR & qPCR. 

Why Partner with Eurogentec? 

k Experience 

k 25 years experience in 

oligonucleotide synthesis 

k Over 16 years experience in 

GMP protein manufacturing 

k Fully trained and skilled staff 

k Quality commitment 

k Organization-wide QMS 

implementation 

k Comprehensive risk analysis 

and mitigation 

k Classified cleanroom facility 

k Supply chain reliability 

k Optimized and validated 

instruments & processes 

k Full traceability for regulatory 

compliance 

k Comprehensive archival 

batch records 

Residual dNA content: 

Diamond Taq ® was 

evaluated using positive 

control (10 ng of E. coli 

DNA) and negative 

control (no DNA). PCR 

products were analyzed 

by gel electrophoresis. 

No detectable E. coli 

DNA was observed 

after PCR on bacterial 

16s DNA. 

DIAMOND Taq ® | DIAGNOSTIC SERVICES 

Introduction 

Choosing a manufacturing partner early in the assay development 

process is an important step towards successful assay validation and 

product commercialization. Eurogentec’s ability to provide a complete 

custom solution makes the difference. 

k Regulatory compliance 

k Full compliance to 

ISO 13485:2003 

k Full compliance to FDA cGMP/ 

QSR (21 CFR Part 820) 

k EU IVD Directive 98/79 EC 

compliance 

k Flexibility - Complete custom 

solutions 

k CMO for manufacturing of 

bulk reagents 

k GMP oligonucleotides and 

master mixes 

k Fill & Finish, labeling 

& packaging

Enzyme Selection Guide 

Page 9 

PCR qPCR Yield 

Recombinant Taq 

k Quality System Management 

k High level of education and training 

k Strict process segregation and employee gowning policy 

k Supplier qualification and evaluation 

k Comprehensive batch records with full traceability 

k IQ/OQ/PQ maintenance and calibration standards 

k Dedicated equipment 

k Stringent document change control procedures 

k Deviation management 

k Process control 

Quality 

Sensitivity 

Low copy 

template 

Specificity 

Difficult 

template 

(GC & AT rich) 

k Exhaustive analytical method validation 

k Critical analysis & documentation rationale 

k Independent QC department for assuring exact specifications prior to release 

k Routine internal audits 

k Customer quality audits 

k Sample retention to facilitate troubleshooting 

k Quality and/or Supply Agreements as extension of the manufacturing contracts to 

clearly define our responsibilities to our partners 

k Project-specific details are discussed and documented. 

k Professional support for manufacturers 

of diagnostic assays 

Long template 

p.5 Diamond Taq ® TAQ-I020-100 *** * ++ ++ ++ * ** 

Hotstart Taq 

p.6 Hot Diamond Taq ® TAQ-I032-100 *** ** +++ +++ +++ *** *** 

p.7 HGS Diamond Taq ® TAQ-I010-100 *** *** +++ +++ +++ ** ** 

* Weakly recommended + Low 

** Recommended ++ Medium 

*** Highly recommended +++ High 

CONFIDENTIALITY AGREEMENT 

This Agreement, effective as of the date of the last signature hereto, is entered into 

BETWEEN 

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Industrial Account Manager� 

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AND 

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CDA Eurogentec/ Company 

WHEREAS 

-03/03/09 

COMPANY and EUROGENTEC (hereinafter referred 

2/4 

to as the “Parties”) are currently 

engaged in discussions with respect to future business and/or technical collaboration in the field of 

the beta-test by COMPANY of IVD cGMP Taq DNA Polymerase for Hotstart PCR applications 

(hereinafter referred to as the “Subject”). 

WHEREAS the Parties expect that such discussions will involve the written or verbal disclosure and 

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WHEREAS the “Disclosing Party” means the Party revealing Information to the other Party, or the 

Party which is the owner. The “Receiving Party” means the Party to whom any Information is 

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Tel.: +32 4 366 61 00 • Fax: +32 4 365 16 04 • E-mail: info@eurogentec.com 

RPM Liège • T.V.A.-(BE)-0427.348.346 

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CDA Eurogentec/ Company -03/03/09 1/4 

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www.eurogentec.com 

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Free 

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15. This Agreement shall be binding upon the PARTIES hereto and their respective successors, 

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# Free samples can be requested at diagnostic.taq@eurogentec.com 

The entire process is performed under controlled conditions; in class 10,000 cleanroom 

for purification (ISO 7 with < 100 cfu/m³) and in class 100 laminar flow hoods for fill 

& finish (ISO 5 in with < 1 cfu/m³). Final product is released by our Quality Assurance 

Department with a formal Certificate of Analysis. Full batch records are maintained and 

can be reviewed during customer or regulatory audits. 

First and foremost, the Eurogentec Diagnostic 

team believes that knowing its customer needs 

and expectations is essential to the success of any 

collaboration. Long-term relationships with our 

customers are built on trust and mutual respect. 

All projects are handled with complete confidentiality 

(CDA). 

9 3

Specifications 

Diamond Taq ® Family 

Parameters Specifications 

Appearance* Colorless solution 

Identity (SDS-PAGE) MW approx. 95 kDa 

Volume activity > 5 U/µl 

Purity (SDS-PAGE) > 98 % 

Performance test: 

PCR - λ DNA* 

Performance test: 

PCR - 18 S DNA* 

Ribonucleases 

(up to 10 U, 1 h, 37 °C) 

Endonucleases 

(up to 30 U, 16 h, 65 °C) 

Exonucleases 

(up to 30 U, 16 h, 65 °C) 

Nicking activity 

(up to 30 U, 16 h, 65 °C) 

Taq 

Diamond Taq ® 

0.5 kb fragment positive 

down to 5 pg 


down to 10 pg 

Not detectable 




E.coli residual DNA < 1 fg / Taq Unit 

Bioburden* ≤ 10 CFU/ml 

Stability* 

Animal-derived additives* None 

24 months (at -20 °C) 

from date of manufacture 

* Also for buffer & MgCl2 Additional HGS Diamond Taq ® specification 

HotStart 

No detectable amplification 

without 95 °C activation 

Additional Hot Diamond Taq ® specification 

Performance test PCR- 

Numb DNA 


down to 10 pg 

+ Chemical modification 

Diamond Taq ® is a highly thermostable enzyme produced and purifed from 

recombinant Escherichia coli bacteria containing the Thermus aquaticus DNA 

polymerase gene. This thermophilic eubacterium strain lacks TaqI restriction 

endonuclease. The expressed enzyme shows very good fidelity and catalyzes 5’k 3’ 

synthesis of DNA with no detectable 3’k 5’ exonuclease activity. The enzyme has 

the 3’ extendase activity allowing TA cloning. 

Diamond Taq ® family enzymes can be ordered & shipped following two 

processes: 

IVD process: Order to diagnostic.taq@eurogentec.com 

k Shipping on dry ice. 

k Full traceability from production, storage to shipment of the product. 

k Tracking number sent to customer the day of the shipment. 

Classical process: Order to order@eurogentec.com 

k Shipping at room temperature*. 

k Full traceability from production to storage of the product. 

IVD 

Process 

Classical 

Process 

+ Eurogentec's proprietary modification 

DIAMOND Taq ® | DIAGNOSTIC SERVICES 

Traceability 

Production Storage Order Shipment Shipping Tracking number 

* Except for Hot Diamond Taq ® 

Product description 

Order & Shipping conditions 

Yes Yes Yes Yes 

(shipment at -20 °C) 

Yes Yes No No 

(shipment at room temperature*) 

HotStart Taq 

HGS Diamond Taq ® 

Hot Diamond Taq ® 

new 

concept 

Yes 

(Sent to customer the day of the shipment) 

No 

¡ Prevents non-specific polymerization 

¡ Recommended for multiplex assays 

¡ Prevents non-specific polymerization 

¡ Universal (long & difficult templates)

500 bp 

Smart 

Smart 

Ladder 

Ladder 

Additional information 

diamond Taq ® 

Product description and intended use 

GoldStar ® Taq DNA Polymerase, originally supplied for Research applications, 

is now manufactured according to a GMP Process resulting, in the Diamond 

Taq ® enzyme that is highly suited for use in in vitro Diagnostic and demanding 

Research PCR & qPCR applications. 

Eurogentec 

Diamond 

IVD-GMP Taq 

Taq ® 

Experimental results 

Competitor 

Competitor 

GMP Taq 

GMP Taq 

DNA (pg): 1000 100 50 5 1000 100 50 5 

k Package content 

Diamond Taq ® is provided at a concentration of 

5 U/µl with 10x reaction buffer and MgCl 2 solution. 

10x Reaction buffer: 750 mM Tris-HCl, 200 mM 

(NH 4 ) 2 SO 4 , 0.1 % (v/v) Tween 20 and stabilizer, 

pH 8.8 (at 19 °C). 

MgCl 2 solution: 25 mM MgCl 2 . 

Enzyme storage buffer: 20 mM Tris-HCl, 1 mM 

DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet 

P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and 

stabilizer pH 8.0 (19 °C). 

Diamond Taq 

Eurogentec (Goldstar ®) 

RUO Taq 

1000 100 50 5 

® was 

evaluated against 

competitor GMP Taq 

for its ability to amplify 

different amounts (from 

5 to 1000 pg) of a 0.5 kb 

PCR template (λDNA). PCR 

products were analyzed by 

gel electrophoresis. 

k Documentation 

Enzymes are provided with Technical Data Sheet & 

Certificate of Analysis with the QC data released 

by a QC authorized person and based on review of 

the complete batch record. 

✔ 

✔ 

Additional Benefits 

Proven performance in many 

applications: Same enzyme as 

extensively validated GoldStar ® . 

Customized Fill & Finish: On request 

Diamond Taq ® enzyme can be provided 

with: 

ê An activity from 5 to 200 U/µl 

ê A glycerol level from 1 to 50% 

diamond Taq ® compared to competitor GMP Taq diamond Taq ® compared to GoldStar ® Taq 

k Diamond Taq ® performance is similar or higher than competitor GMP Taq 

www.eurogentec.com 

500 bp 

Smart 

Smart 

Ladder 

Ladder 


Diamond 

IVD-GMP Taq 

Taq ® 

DNA (pg): 

1000 100 50 5 1000 100 50 5 

k Same enzymes 

k Same performances 

k Higher qualities 

k Storage conditions 

Storage at -20 °C is recommended. 

9 5 

See also general benefits page 2 

Diamond Taq ® was evaluated 

against Eurogentec’s 

GoldStar ® Competitor 

Eurogentec (Goldstar ®) 

Eurogentec (Goldstar 

GMP Taq 

RUO Taq 

Taq for its ability 

to amplify different amounts 

(from 5 to 1000 pg) of a 0.5 kb 

PCR template (λDNA). PCR 

products were analyzed by 


® ) 

RUO Taq 

1000 100 50 5

Fluorescence (dRn) 

0.3 

0.2 

0.1 

0.0 


✔ 

✔ 

✔ 


Universal: Amplification of long & 

difficult templates. 

Specific: Prevents non-specific 

polymerization as primer-dimer 

formation and increases the PCR yield of 

specific products. 

Very short activation time: Minimum 

20 seconds. 

EXPERIMENTAL RESULTS 

A ctivation time 

H ot Diamond T aq® shows no amplification at room temperature 

and gives very high yield of specific product. The enzyme needs 

very short activation time (100% activated during the first PCR 

cycle) but is compatible with all existing protocols, from 20 sec. to 



Hot Diamond Taq ® is provided at a concentration of 

5 U/µl with 10x reaction buffer and MgCl 2 solution. 


(NH 4 ) 2 SO 4 , 0.1 % (v/v) Tween 20 and stabilizer, pH 

8.8 (at 19 °C). 

MgCl 2 solution: 25 mM MgCl 2 




stabilizer, pH 8.0 (19 °C). 

DiamonD Taq ® | DiagnosTic services 

Hot diamond Taq ® 


The enzyme exhibits unique HotStart characteristics and represents a completely 

new “HotStart concept”. HotStart characteristics are neither accomplished 

through chemical modification nor a blocking antibody but a proprietary agent 

which prevents non-specific polymerization; thereby preventing primer-dimer 

formation and increasing the PCR yield of specific products. Hot Diamond Taq ® 

shows no amplification at room temperature and gives very high yield of specific 

products. The enzyme needs very short activation time (100 % activated during 

the first PCR cycle) but is compatible with all existing protocols (from 20 seconds 

to 15 minutes at 95 °C). 

Experimental results 

Activation time Hot diamond Taq ® 15 minutes at 95°C. 

compared to HotStart competitors 

DNA(pg): 

20 sec. 5 min. 10 min. 

20 sec. 5 min 10 min 

5 1 0 5 1 0 5 1 0 

l Hot Diamond Taq ® needs very short activation time 

2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 

36 38 40 41 42 

Hot Diamond Taq ® was 

evaluated using from 

20 secondes to 10 minutes as 

activation time for its ability to 

amplify 1 and 5 pg of λ DNA. 

PCR products were analyzed by 


High quality results 

obtained using 

the Hot Diamond 

Taq ® (twice serial 

dilutions) on ß-actin 

cDNA performed in 

singleplex Taqman 

assay. 



Certificate of Analysis with the QC data released by 

a QC authorized person and based on review of the 

complete batch record. 

Performance test on difficult template: 

gDNA NUMB (306b) 

HDT C1 C2 C3 C4 DT 

Performance test on GC rich template: 

SCO3449 (152b) – 72 % GC 

HDT 

C1 C2 

C3 

C4 

DT 

l Hot Diamond Taq ® is highly suitable for 

difficult and GC rich template amplifications 



Hot Diamond Taq ® 

(HDT) & Diamond 

Taq ® (DT) were 

evaluated against 

HotStart competitors 

(C1, 2 & 4 – Chemical 

modification; C3 - 

Antibody) for their 

ability to amplify 

difficult and GC 

rich templates. 

PCR products were 

analyzed by gel 

electrophoresis.

HGS diamond Taq ® 


HotGoldStar ® Taq DNA Polymerase, originally supplied for Research applications, 

is now manufactured according to a GMP Process resulting in the HGS Diamond 

Taq ® enzyme that is highly suited for use in in vitro Diagnostic & demanding 

Research PCR & qPCR applications. 

HGS Diamond Taq ® is a chemically modified HotStart Taq DNA Polymerase 

requiring a thermal activation of 10 minutes at 95 °C to reach maximal initial 

activity. Before activation the enzyme completely lacks any activity below 74 °C. 

This avoids non-specific priming at low temperature. During the PCR, the rest of 

its activity is released. HGS Diamond Taq ® is more heat-stable than commonly 

used Taq DNA Polymerases. 

DNA fragments as long as 2 kb can be efficiently amplified. HGS Diamond Taq ® 

DNA Polymerase provides efficient amplification of specific products without 

amplifying non-specific products or primer dimers. 

HGS diamond Taq ® compared to Hot GoldStar ® Taq HGS diamond Taq ® 


l Same enzymes 

l Same performances 

l Higher qualities 



HotGoldStar ® 


HGS Diamond Taq ® DNA Polymerase is provided at 

a concentration of 5 U/µl with 10x reaction buffer 

and MgCl 2 solution. 


KCI and stabilizer, pH 8.5 (at 19 °C). 

MgCl 2 solution: 25 mM MgCl 2 . 




stabilizer pH 8.0 (19 °C). 


was evaluated against 

Eurogentec’s HotGoldStar ® 

(HGS) for its ability to 

amplify in triplicate 5 pg 

of a 0.5 kb PCR template 

(λ DNA). PCR products 

were analyzed by gel 

electrophoresis. 



Certificate of Analysis with the QC data released by 

a QC authorized person and based on review of the 

complete batch record. 

Fluorescence (∆Rn) 

0.3 

0.2 

0.1 

0.0 

✔ 

✔ 

✔ 

20 sec. 5 min. 10 min. 


Specific: Prevents non-specific 

polymerization as primer-dimer 

formation and increases the PCR yield of 

specific products. 

Proven performance in many 

applications: Same enzyme as EGT's 

extensively validated HotGoldStar ® . 

Customized Fill & Finish: On request 

HGS Diamond Taq ® enzyme can be 

provided with: 

ê An activity from 5 to 50 U/µl 

2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 

ê A glycerol level from 1 to 50 % 

9 7 


HGS Diamond Taq ® was 

evaluated for its ability to 

amplify a PCR template 

of 0.5 kb (λDNA) for 

several activation 

times. PCR products 

were analyzed by gel 

electrophoresis. 

l HGS Diamond Taq ® needs 5 min. activation time to amplifly DNA fragment 



High quality results 

obtained using 

the HGS Diamond 

Taq ® (twice serial 

dilutions) on ß-actin 

cDNA performed in 

singleplex Taqman 

assay.

DIA-TAQ-FAMILY-0911-US-V1 

Eurogentec, your manufacturing partner of choice 

For inquiries, please contact the Eurogentec Diagnostic Taq team: 

North America 

Tel.: +1 858 793 2661 

Fax: +1 858 793 2666 

diagnostic.taq.na@eurogentec.com 

11111 Flintkote Ave. - San Diego - CA 92121-1222 

k Hot diamond Taq ® products 

Hot Diamond Taq ® products are covered by international EUROGENTEC pending Patent 

application (PCT/EP2008/067435). Hot Diamond Taq ® products are sold exclusively 

for Research use only by the purchaser and may not be used for clinical or diagnostic 

purposes, resold, distributed or re-packaged without the prior written consent of 

EUROGENTEC S.A. It may be necessary to obtain a separate license for certain 

patented applications in which the Hot Diamond Taq ® products are used. 

Europe 

Tel.: +32 4 372 74 00 

Fax: +32 4 372 75 00 

diagnostic.taq@eurogentec.com 

5, Rue Bois Saint-Jean - 4102 Seraing - Belgium 

k Roche Enzyme Products Conveying Polymerase Patent Rights 

Use of this product is covered by one or more of the following US patents and 

corresponding patent claims outside the US: 5,789,224, 5,618,771, 6,127,155 and claims 

outside the US corresponding to expired US Patent No. 5,079,352.The purchase of this 

product includes a limited, non-transferable immunity from suit under the foregoing 

patent claims for using only this amount of product for the purchaser’s own internal 

research. No right under any other patent claim, no right to perform any patented 

method, and no right to perform commercial services of any kind, including without 

limitation reporting the results of purchaser’s activities for a fee or other commercial 

consideration, is conveyed expressly, by implication, or by estoppel. This product is for 

research use only. Diagnostic uses under Roche patents require a separate license 

from Roche. Further information on purchasing licenses may be obtained by contacting 

the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, 

California 94404, USA. 

k Chemically-Modified Hot-Start Polymerase Reagents and Kits 

Use of this product is covered by one or more of the following US patents and 

corresponding patent claims outside the US: 6,127,155 5,677,152 (claims 1 to 23 only), 

5,773,258 (claims 1 and 6 only), and claims outside the US corresponding to expired US 

Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable 

immunity from suit under the foregoing patent claims for using only this amount of 

product for the purchaser’s own intemal research. No right under any other patent 

claim and no right to perform commercial services of any kind, including without 

limitation reporting the results of purchaser’s activities for a fee or other commercial 

consideration, is conveyed expressly, by implication, or by estoppel. This product is for 

research use only. Diagnostic uses under Roche patents require a separate license 

from Roche. Further information on purchasing licenses may be obtained by contacting 

the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, 

California 94404, USA.

diamond Taq® Product description - Eurogentec

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