FOX3/NeuN Monoclonal Antibody, Purified - Eurogentec
FOX3/NeuN Monoclonal Antibody, Purified - Eurogentec
FOX3/NeuN Monoclonal Antibody, Purified - Eurogentec
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Description:<br />
Mouse <strong>Monoclonal</strong> <strong>Antibody</strong> to Fox3/<strong>NeuN</strong><br />
Also Known As:<br />
RNA binding protein fox-1 homolog 3; fox-1<br />
homolog C; neuronal nuclei;<br />
hexaribonucleotide binding protein 3<br />
Intended Use:<br />
**Research Use Only (RUO)**<br />
This product is sold for laboratory research<br />
use only, not for human or in-vivo use.<br />
Form:<br />
<strong>Purified</strong> <strong>Antibody</strong> (in PBS)<br />
Clone:<br />
MCS-1B7<br />
Host:<br />
Mouse<br />
IsoType:<br />
IgG2b<br />
Species Reactivity:<br />
Human, cow, pig, mouse, rat and other<br />
mammals<br />
[Ab]:<br />
1 mg/mL<br />
<strong>FOX3</strong>/<strong>NeuN</strong> <strong>Monoclonal</strong> <strong>Antibody</strong>,<br />
<strong>Purified</strong><br />
Catalog Number: SIG-39860<br />
Available Size: 0.1 mL<br />
Specificity:<br />
Fox3 is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the<br />
name Fox is an acronym of "Feminizing locus on X". The Fox protein and its 3 mammalian homologues are all about 46kDa proteins each of<br />
which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of<br />
4 beta strands and two alpha-helices. Fox3 is a protein which has a function in RNA splicing and is expressed heavily and specifically in<br />
neuronal nuclei. Our antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. The<br />
MCA-1B7 antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/<strong>NeuN</strong> and the complete absence of staining of astrocytes<br />
and other kinds of non-neuronal cells. This Fox3/<strong>NeuN</strong> antibody is therefore an excellent marker of neuronal cells.<br />
Uses:<br />
This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC).<br />
Suggested Working Dilution:<br />
The optimal working dilution should be determined for each specific assay condition.<br />
• WB: 1:500- 1:1,000<br />
• IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250) or polymer based<br />
detection systems such as ACUITYAdvanced Biotin Free Polymer Detection (SIG-32902).<br />
Tissue Sections: Formalin Fixed Paraffin Embedded sections, Frozen sections<br />
Pre-treatment: not required<br />
Incubation: 60min at room temperature<br />
• ICC: 1:500- 1:1,000<br />
Notes:<br />
Positive Control: Brain tissue<br />
Predicted MW: 46 & 48 kDa<br />
See reverse side for additional information Page 1 of 2<br />
<strong>Antibody</strong> Products:<br />
Email:<br />
+1.800.922.2226 • +1.510.594.3800<br />
ab.products@covance.com www.covance.com
<strong>Antibody</strong> Products:<br />
Email:<br />
+1.800.922.2226 • +1.510.594.3800<br />
ab.products@covance.com www.covance.com<br />
<strong>FOX3</strong>/<strong>NeuN</strong> <strong>Monoclonal</strong> <strong>Antibody</strong>, <strong>Purified</strong><br />
Catalog Number: SIG-39860<br />
Storage:<br />
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing<br />
the antibody. For long-term storage, keep the antibody at -80°C.<br />
References:<br />
Hodgkin J, Zellan JD, Albertson DG. Identification of a candidate primary sex determination locus, fox-1, on the X chromosome of<br />
Caenorhabditis elegans. Development 120:3681-3689, 1994.<br />
Mullen RJ, Buck CR, Smith AM. <strong>NeuN</strong>, a neuronal specific nuclear protein in vertebrates. Development 116:201-211, 1994.<br />
Herculano-Houzel S, Lent R. Isotropic fractionator: a simple, rapid method for the quantification of total cell and neuron numbers in the brain. J<br />
Neurosci 25:2518-21, 2005.<br />
Kim KK, Adelstein RS, Kawamoto S. Identification of neuronal nuclei (<strong>NeuN</strong>) as Fox-3, a new member of the Fox-1 gene family of splicing<br />
factors. J Biol Chem 284:31052-31061, 2009.<br />
Underwood JG, Boutz PL, Dougherty JD, Stoilov P, Black DL. Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing<br />
regulators in mammals. Mol Cell Biol 25:10005-10016, 2005.<br />
Warranty/Conditions:<br />
Covance products may not be resold or modified for resale without prior written approval. All sales are subject to Covance <strong>Antibody</strong> Products<br />
Terms and Conditions of Sale.<br />
Datasheet Revision Date:<br />
12/7/2011<br />
Related Products:<br />
▪ ACUITYAdvanced Biotin Free Polymer Detection System 50 Test DAB kit Catalog Number SIG-32902<br />
▪ Ultra Streptavidin (USA) Horseradish peroxidase (HRP) 50 Test, Multi-Species, DAB Detection Kit Catalog Number SIG-32250<br />
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