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(SMI 37) Monoclonal Antibody - Eurogentec

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Description:<br />

<strong>Monoclonal</strong> <strong>Antibody</strong> against Non-<br />

Phosphorylated Neurofilament H<br />

Intended Use:<br />

**Research Use Only (RUO)**<br />

This product is sold for laboratory<br />

research use only, not for human or invivo<br />

use.<br />

Form:<br />

Ascites Fluid (contains 0.01M sodium<br />

azide)<br />

Clone:<br />

<strong>SMI</strong>-<strong>37</strong><br />

Host:<br />

Mouse<br />

IsoType:<br />

IgM<br />

Species Reactivity:<br />

Mammalian<br />

Neurofilament H Non-Phosphorylated<br />

(<strong>SMI</strong> <strong>37</strong>) <strong>Monoclonal</strong> <strong>Antibody</strong><br />

Catalog Number: <strong>SMI</strong>-<strong>37</strong>R<br />

Available Size: 0.1 mL, 0.5 mL<br />

Specificity:<br />

<strong>SMI</strong> <strong>37</strong> reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. Immunocytochemically, <strong>SMI</strong><br />

<strong>37</strong> visualizes some neuronal cell bodies, dendrites and thick axons in the central and peripheral nervous systems. <strong>SMI</strong> <strong>37</strong><br />

staining is more restricted than that seen with <strong>SMI</strong> 32 or <strong>SMI</strong> 38 and Neuronal cell bodies are intensely stained while neuronal<br />

processes staining is less prominent. This IgM antibody reacts with goat antimouse IgG and mouse ClonoPAP® via the light<br />

chains common to IgG and IgM.<br />

Uses:<br />

This antibody is effective in immunoblotting (WB), immunocytochemistry, immunohistochemistry (IHC) and ELISA.<br />

Suggested Working Dilution:<br />

The optimal working dilution should be determined for each specific assay condition.<br />

• WB: 1:1,000<br />

• IHC: 1:1,000<br />

• ELISA: 1:1,000<br />

The extent of permissible dilution of <strong>SMI</strong> <strong>37</strong> beyond those recommended for general application depends upon nature and<br />

concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.<br />

www.covance.com<br />

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Storage:<br />

Store at -20°C. Upon initial thawing, apportion into working<br />

aliquots and store at -20°C. Avoid repeated freeze-thaw<br />

cycles to prevent denaturing the antibody. For long-term<br />

storage, keep the antibody at -80°C.<br />

Warranty/Conditions:<br />

Covance products may not be resold or modified for resale without prior<br />

written approval.All sales are subject to Covance <strong>Antibody</strong> Products<br />

Terms and Conditions of Sale.<br />

Datasheet Revision Date:<br />

03/13/2011<br />

<strong>Antibody</strong> Products:<br />

Email:<br />

+1.800.922.2226<br />

+1.510.594.3800<br />

ab.products@covance.com<br />

Neurofilament H Non-Phosphorylated (<strong>SMI</strong> <strong>37</strong>) <strong>Monoclonal</strong> <strong>Antibody</strong><br />

Catalog Number: <strong>SMI</strong>-<strong>37</strong>R<br />

www.covance.com<br />

Related Products:<br />

• Ultra Streptavidin (USA) Horseradish peroxidase (HRP) 50<br />

Test, Multi-Species, AEC Detection Kit (SIGNET), Catalog<br />

Number: SIG-32248<br />

• Pan-Neuronal Neurofilament Marker (<strong>SMI</strong> 311) <strong>Monoclonal</strong><br />

<strong>Antibody</strong>, Catalog Number: <strong>SMI</strong>-311R<br />

• Normal Goat <strong>Antibody</strong>, Catalog Number: <strong>SMI</strong>-6020C<br />

• Ultra Streptavidin (USA) Horseradish peroxidase (HRP) 50<br />

Test, Multi-Species, DAB Detection Kit (SIGNET), Catalog<br />

Number: SIG-32250<br />

• Mouse ClonoPAP®, Catalog Number: <strong>SMI</strong>-4050L<br />

• Neurofilament H Non-Phosphorylated (<strong>SMI</strong> 32) <strong>Monoclonal</strong><br />

<strong>Antibody</strong>, Catalog Number: <strong>SMI</strong>-32R<br />

• Neurofilament H & M Non-Phosphorylated (<strong>SMI</strong> 33) <strong>Monoclonal</strong><br />

<strong>Antibody</strong>, Catalog Number: <strong>SMI</strong>-33R<br />

• Neurofilament H Non-Phosphorylated (<strong>SMI</strong> <strong>37</strong>) <strong>Monoclonal</strong><br />

<strong>Antibody</strong>, Catalog Number: <strong>SMI</strong>-<strong>37</strong>R<br />

• Neurofilament H Non-Phosphorylated (<strong>SMI</strong> 38) <strong>Monoclonal</strong><br />

<strong>Antibody</strong>, Catalog Number: <strong>SMI</strong>-38R<br />

• Neurofilament H Non-Phosphorylated (<strong>SMI</strong> 39) <strong>Monoclonal</strong><br />

<strong>Antibody</strong>, Catalog Number: <strong>SMI</strong>-39R<br />

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