Neurofilament NF-H Polyclonal Antibody, Purified - Eurogentec

Description:
Polyclonal Antibody against
Neurofilament NF-H
Form:
Purified Antibody
Host:
Chicken
IsoType:
IgY
Species Reactivity:
Human, Rat, Mouse, Cat, Chicken
Neurofilament NF-H Polyclonal Antibody,
Purified
Catalog Number: PCK-592P
Available Size: 0.05 mL
Rat hippocampus stained with rabbit anti-GFAP, PRB-571C
(red, AF568) and chicken anti-NF-H, PCK-592P (green,
AF488). Photo courtesy of Molecular Expressions.
Specificity:
Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron
microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions
from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide
structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of
impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins,
known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in
some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are
among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
This antibody was raised in chickens against purified bovine NF-H neurofilaments.
Uses:
This antibody is effective in immunoblotting and immunofluorescence. This antibody does not bind to protein A or protein G.
Suggested Working Dilution:
The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory
research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior
written approval.
• Western blot: 1:1,000,000*
• Immunofluorescence: 1:100,000
www.covance.com
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Notes:
* Predicted MW = 200-220kD
Storage:
Store at -20°C. Upon initial thawing, apportion into working
aliquots and store at -20°C. Avoid repeated freeze-thaw
cycles to prevent denaturing the antibody. For long-term
storage, keep the antibody at -80°C.
Warranty/Conditions:
Covance products may not be resold or modified for resale without prior
written approval.All sales are subject to Covance Antibody Products
Terms and Conditions of Sale.
Datasheet Revision Date:
03/13/2011
Antibody Products:
Email:
+1.800.922.2226
+1.510.594.3800
ab.products@covance.com
www.covance.com
Related Products:
Neurofilament NF-H Polyclonal Antibody, Purified
Catalog Number: PCK-592P
• Neurofilament NF-H Monoclonal Antibody, Catalog Number:
MMS-585R
Rat cortical neuron stained with NF-H chicken (PRB-592P)
antibody, diluted 1:100,000. Photo courtesy of EnCor
Biotechnology Inc.
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