Analytical Chemistry Chemical Cytometry Quantitates Superoxide

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Figure 2. (A) ESI-LTQ-CID-MS 2 product ion spectrum of the type 1 modified PPARR peptide (aa 195-209) [M + 2H + BuUrBu] 2+ at m/z 945.47. (B) ESI-LTQ-MS 3 product ion spectrum of [M + 2H + BuUr] 2+ at m/z 902.94. $: signal not assigned. and, as such, originate from cleavage of the amide bond between the lysine ε-amine group and the cross-linker. Offline Nano-HPLC/MALDI-TOF/TOF Mass Spectrometry. To ensure and to probe the general applicability of 1 for both ESI- and MALDI-MS, we additionally examined the frag- (39) Müller, M. Q.; de Koning, L. J.; Schmidt, A.; Ihling, C.; Syha, Y.; Rau, O.; Mechtler, K.; Schubert-Zsilavecz, M.; Sinz, A. J. Med. Chem. 2009, 52, 2875–2879. 6964 Analytical Chemistry, Vol. 82, No. 16, August 15, 2010 mentation behavior of the cross-linker in the higher kinetic energy regime of collision activation (kinetic energy of the precursor ion in TOF/TOF-CID Ekin ≈ 1 keV) 40-42 in MALDI-TOF/TOF (ISD and CID) experiments. In the same fashion as for low-energy ESI- LTQ-orbitrap-MS n product experiments (kinetic energy of the precursor ion in LTQ-CID Ekin ≈ 1 eV) 43,44 Munc13-1 and PPARR peptides were created by tryptic digestion and separated by nano- HPLC before MS analysis.
Figure 3. (A) ESI-LTQ-CID-MS 2 product ion spectrum of the triply protonated type 2 modified Munc13-1 peptide (amino acids 3-9 connected with amino acids 10-15) at m/z 597.01. (B, C) ESI-LTQ-MS 3 product ion spectra of the modified R peptide [R +2H + Bu] 2+ at m/z 463.76 (B) and of the � peptide [� + H + Bu] + at m/z 819.48 (C). Peptides Modified with Hydrolyzed Linkers (Type 0). As with collision activation in the low-energy regime in an LTQ-CID- MS 2 product ion experiment of the type 0 modified Munc13-1 peptide (Figure 1A), the MALDI-TOF/TOF-CID spectrum exhibits the 26 u doublet of product ions 6 and 7 as base peaks resulting from the highly preferential cleavage of the urea crosslinker (Figure 4, Table 1). However, due to the overall increased (40) Pittenauer, E.; Allmaier, G. Comb. Chem. High Throughput Screening 2009, 12, 137–155. (41) Medzihradszky, K. F.; Campbell, J. M.; Baldwin, M. A.; Falick, A. M.; Juhasz, P.; Vestal, M. L.; Burlingame, A. L. Anal. Chem. 2000, 72, 552–558. (42) Shenar, N.; Sommerer, N.; Martinez, J.; Enjalbal, C. J. Mass Spectrom. 2009, 44, 621–632. (43) Schwartz, J. C.; Senko, M. W.; Syka, J. E. J. Am. Soc. Mass Spectrom. 2002, 13, 659–669. (44) Douglas, D. J. Mass Spectrom. Rev. 2009, 28, 937–960. collision activation in the TOF/TOF-CID experiment (Figure 4) compared to the LTQ-CID experiment (Figure 1A), a significant series of b and y ion signals resulting from the cleavage of peptide backbone amide bonds were additionally observed in the former. ISD-MS/MS data of primary product ions observed in Figure 4 for signals at m/z 943.6 (corresponding to a CNL of 129 u) and m/z 969.6 (corresponding to a CNL of 103 u) are comparable to those of the fragmentation product of a Munc13-1 cross-link, in which Lys-4 is connected with Lys-13 (Figure 3). Also, backbone fragmentation of the peptide was observed in MALDI-ISD experiments. MALDI-TOF/TOF data of a PPARR peptide comprising amino acids 259-272 exhibit a fragmentation behavior analogous to that in an ESI-CID-MS 2 experiment (Supporting Information, Figure S3). Analytical Chemistry, Vol. 82, No. 16, August 15, 2010 6965
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Anal. Chem. 2010, 82, 6745-6750 Let
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purchased from Invitrogen-Molecular
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Figure 3. Electropherograms of TPP-
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Anal. Chem. 2010, 82, 6751-6755 Res
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(pH 8.0) with cysteine and cystamin
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Figure 4. Ion mobility mass spectra
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GOx glucose + O298 gluconic acid +
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coater at 2000 rpm for 20 s, and th
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Figure 5. Comparison of cyclic volt
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in channels with either no grooves
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indicators of atmospheric processin
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Figure 1. GC/MS total ion chromatog
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Table 2. Concentrations and Stable
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on a substrate are preferred. 20-24
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Figure 4. SERS analysis of NAADP co
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Anal. Chem. 2010, 82, 6775-6781 Hig
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tion, 2 µL of proprionaldehyde wer
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Figure 4. Analysis of 2a by HPLC-MS
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eaction of 1a with PBH can be condu
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Numerous references had demonstrate
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Figure 1. TEM images of the prepare
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Figure 4. Schematic representation
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esult in a big SPR signal change wi
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also reduces chemical noise, which
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Table 1. Extraction Yields, Liquid
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scanning of AMPP amides of the anal
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Anal. Chem. 2010, 82, 6797-6806 δ
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Figure 1. Schematic view of the pre
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from the specimen and enclosed in a
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Figure 4. (A) IRMS mass-44 chromato
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Table 3. Ambient Measurement Result
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Anal. Chem. 2010, 82, 6807-6813 Dir
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Polymerase (1 U per sample). Reacti
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of which was constant for all ampli
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analytically useful signals at less
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carbon black and RP-C18 for the ext
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solution in an equal volume, and 1
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Table 2. Concentrations and Ratios
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Anal. Chem. 2010, 82, 6821-6829 Mac
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mg/mL protein, followed by separati
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Figure 2. Productivity of SEQUST an
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Figure 4. High-resolution MS/MS spe
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Figure 6. Characterization of PSMs
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containing T-T mismatches. 23 Based
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Figure 1. Extinction spectra of sol
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Figure 3. (A) The value of Ex650 nm
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Figure 5. Extinction spectra and co
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wished to explore the dehydration o
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constant medium for separation; we
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Figure 2. Standards of (Pi)n, n ) 1
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Figure 5. Quantitative calibration
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Anal. Chem. 2010, 82, 6847-6853 Met
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Figure 1. 226 Ra spectrum by liquid
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Table 1. Counting Properties and De
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Table 3. Analysis of 226 Ra in Sedi
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mercial microarray scanner and fabr
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Figure 2. Optical transmission meas
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Figure 5. Volcano plots detailing t
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data as well. The 41 genes in Table
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corresponding compound if its chemi
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Figure 1. Schematic illustration of
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Table 1. Absolute Quantification Re
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ment. Therefore, the long-time drea
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Figure 1. Chemically actuated micro
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Figure 2. Influence of a surfactant
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Figure 5. Device to eject and mix s
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Anal. Chem. 2010, 82, 6877-6886 Imm
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NaCl, phosphate buffer saline (PBS)
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Figure 2. Product ion mass spectra
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-70 °C resolved the problem, givin
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Table 1. Intraday Precision and Acc
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Anal. Chem. 2010, 82, 6887-6894 Fer
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Figure 1. Infrared spectra of (A) u
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Figure 3. Cyclic voltammograms obta
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Figure 6. Calculated charge from ch
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Anal. Chem. 2010, 82, 6895-6903 Ele
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Table 1. Chemical Structure, pKa Va
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pH with a tilted baseline (Figure 1
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Table 2. Linearity and Detection Li
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ascorbic acid (AA), uric acid (UA),
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the multielement capabilities, the
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RESULTS AND DISCUSSION Sulfur Detec
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Table 2. Molecular Properties and C
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Anal. Chem. 2010, 82, 6911-6918 Dir
Page 169 and 170: dilution and hybridization buffer.
Page 171 and 172: solution under appropriate incubati
Page 173 and 174: Figure 4. Standardization curve for
Page 175 and 176: Anal. Chem. 2010, 82, 6919-6925 Ele
Page 177 and 178: the ×10 objective, to have a large
Page 179 and 180: Figure 2. With a suitable removal o
Page 181 and 182: the NB signal in a much better foot
Page 183 and 184: Scheme 1. Reactions of Selenium Rea
Page 185 and 186: Figure 2. (a) ESI-MS spectrum showi
Page 187 and 188: Figure 4. (a) ESI-MS spectrum showi
Page 189 and 190: Anal. Chem. 2010, 82, 6933-6939 Dif
Page 191 and 192: trode 28 by a finite element using
Page 193 and 194: Figure 4. Comparison between simula
Page 195 and 196: Figure 6. Comparison between simula
Page 197 and 198: educed in the vicinity of double bo
Page 199 and 200: Figure 2. Normalized product ion ab
Page 201 and 202: Figure 4. EID (a) and IRMPD (b) of
Page 203 and 204: Anal. Chem. 2010, 82, 6947-6957 Ide
Page 205 and 206: Figure 1. Schematic flowchart showi
Page 207 and 208: difference, ppm compound Table 1. I
Page 209 and 210: isoforms, its successful use, in th
Page 211 and 212: Figure 5. Extracted ion current ESI
Page 213 and 214: m/z 1172.935 was observed for Ser14
Page 215 and 216: linked products via affinity tags.
Page 217 and 218: Scheme 2. Fragmentation Mechanism o
Page 219: Figure 1. (A) ESI-LTQ-CID-MS 2 prod
Page 223 and 224: Figure 5. (A) MALDI-TOF/TOF product
Page 225 and 226: Anal. Chem. 2010, 82, 6969-6975 Ana
Page 227 and 228: Figure 3. Equilibrium response as a
Page 229 and 230: Figure 6. The average measured resp
Page 231 and 232: for the fill time, and we find that
Page 233 and 234: nucleotide tails. 3-5 Thus, the amo
Page 235 and 236: allow the use of higher aptamer con
Page 237 and 238: quent ligation of the aptamers afte
Page 239 and 240: Anal. Chem. 2010, 82, 6983-6990 Imp
Page 241 and 242: Figure 2. Configuration editor wind
Page 243 and 244: Figure 4. Dependencies between volu
Page 245 and 246: Since the time for liquid expulsion
Page 247 and 248: Anal. Chem. 2010, 82, 6991-6999 Int
Page 249 and 250: Figure 1. Representation of the Car
Page 251 and 252: Table 2. Capillary Electrophoresis
Page 253 and 254: Figure 4. (A) Typical raw electroph
Page 255 and 256: field. DNA profiles were delivered
Page 257 and 258: Another approach to handle this lim
Page 259 and 260: (principal components, PCs) in whic
Page 261 and 262: Figure 5. Relevant score plots and
Page 263 and 264: CONCLUSIONS In this paper we have i
Page 265 and 266: electroactive-species loaded liposo
Page 267 and 268: Figure 2. Qdot-based FLFTS response
Page 269 and 270: Figure 6. Fluorescence imaging of Q
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Anal. Chem. 2010, 82, 7015-7020 Sel
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Table 1. Effect of 1 D-LC Condition
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Figure 3. Peak-production rate vers
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Anal. Chem. 2010, 82, 7021-7026 Cel
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luciferase (T7 control vector) was
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Figure 3. Inhibitory effects of luc
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Anal. Chem. 2010, 82, 7027-7034 Pat
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Electrochemistry. Electrochemical m
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Figure 2. SEM images of Au substrat
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Table 3. Film Thickness Measurement
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Anal. Chem. 2010, 82, 7035-7043 Qua
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Figure 1. (A) FL spectra of BSPOTPE
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Figure 4. (A) Variation in the FL i
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Figure 6. (A) FL spectra of BSPOTPE
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Scheme 1. Proposed Mechanism for Fl
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one or more drawbacks including poo
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Figure 2. Free fluorophores do not
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Anal. Chem. 2010, 82, 7049-7052 Dev
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Figure 2. Comparative analysis of d
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