Analytical Chemistry Chemical Cytometry Quantitates Superoxide

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Table 2. Amino Acid and Elemental Composition of Pig Kidney Metallothioneins MTs (under demetalation conditions) complexed MTs (under metalation conditions) mass difference (demetalatedcomplexed) time, m/z, z ) 5, most abundant most abundant difference, amino acid m/z, z ) 5, most abundant most abundant difference, min measured mass, measured mass, theoretical ppm composition measured mass, measured mass, theoretical ppm 1 33.8 1189.032 5940.124 5940.128 0.7 M1D2P3N1C20S9A7T3G6K6R1Q1I1 1343.877 6714.349 6714.342 -1.0 7Cd - 14H 2 35 1189.837 5944.149 5944.159 1.7 M1D2P2N1C20S10A6T3G6K7R1V2 1344.685 6718.389 6718.374 -2.2 7Cd - 14H 3 37.4 1197.437 5982.149 5982.138 -1.8 Ac-M1D2P3N1C20S9A7T3G6K6R1Q1I1 1352.280 6756.364 6756.353 -1.6 7Cd - 14H 4 38.1 1194.840 5969.164 5969.163a -0.1 M1D2P3N1C20S9A7T3G6K5R2Q1I1 1349.482 6742.374 6742.377 0.5 7Cd - 14H M1D3P2 N1C20S8A7T2G6K8Q1I1V1 5 38.5 1198.243 5986.179 5986.170 -1.4 Ac-M1D2P2N1C20S10A6T3G6K7R1V2 1353.086 6760.394 6760.384 -1.4 7Cd - 14H 6 40.5 1203.246 6011.194 6011.190 -0.6 Ac-M1D3P2N1C20S8A7T2G6K8Q1I1V1 1358.090 6785.414 6785.405 -1.3 7Cd - 14H 7 41 1203.036 6010.1436 6010.1440 0.1 Ac-M1D2P3N1C20S9A7T3G6K5R2Q1I1 1357.884 6784.384 6784.359 -3.6 7Cd - 14H 8 44.3 1272.167 6355.799 6355.792b -1.0 Ac-M1D3P2N1C20S8A7T2G6K8Q1I1V1 1360.478 6797.354 6797.344 -1.4 4Cd - 8H form N° 6954 Analytical Chemistry, Vol. 82, No. 16, August 15, 2010 a Theoretical mass for a mixture of MT1a/MT2a (1/1.65). b Mass of N-AcMT2a after thiols oxidation (formation of one disulfide bridge) + 2Cd(II) + 2Cu(I) - 6H. (Figure 4d) showed a number of fairly intense quite well resolved peaks in the 33-45 min range which corresponded to the peaks in the ICP MS chromatograms. The mass spectrometric data allowed spectra of MT-like ions ([M+5H] 5+ ) to be detected at retention times corresponding to the ICP MS peaks as demonstrated in Figure 5. In seven cases a mass spectrum of the apo-isoform and a corresponding mass spectrum of the Cd7-metalated isoform could be found resulting in a XIC with a single intense peak. In the case of peak 8 (Figure 4b), no apo-isoform could be detected under acidic conditions; the isotopic pattern of the peak revealed the presence of metals. The elemental composition calculated on the basis of the accurate molecular mass indicated the presence of two atoms of Cd and two atoms of Cu. The mass spectrometric data are shown in Table 2. As it can be seen the mass accuracies fitting a tentative amino acid composition are usually
Figure 5. Extracted ion current ESI MS chromatograms of MTs subisoforms of pig kidney cell line with mass spectra in insets (upper: metalated subisoform; lower: subisoform under acidic conditions) at retention time (a) 33.8 min, (b) 35 min, (c) 37.4 min, (d) 38.1 min, (e) 38.5 min, (f) 40.5 min, (g) 41 min, (h) 44.3 min. Analytical Chemistry, Vol. 82, No. 16, August 15, 2010 6955
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Anal. Chem. 2010, 82, 6745-6750 Let
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purchased from Invitrogen-Molecular
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Figure 3. Electropherograms of TPP-
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Anal. Chem. 2010, 82, 6751-6755 Res
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(pH 8.0) with cysteine and cystamin
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Figure 4. Ion mobility mass spectra
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GOx glucose + O298 gluconic acid +
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coater at 2000 rpm for 20 s, and th
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Figure 5. Comparison of cyclic volt
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in channels with either no grooves
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indicators of atmospheric processin
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Figure 1. GC/MS total ion chromatog
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Table 2. Concentrations and Stable
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on a substrate are preferred. 20-24
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Figure 4. SERS analysis of NAADP co
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Anal. Chem. 2010, 82, 6775-6781 Hig
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tion, 2 µL of proprionaldehyde wer
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Figure 4. Analysis of 2a by HPLC-MS
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eaction of 1a with PBH can be condu
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Numerous references had demonstrate
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Figure 1. TEM images of the prepare
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Figure 4. Schematic representation
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esult in a big SPR signal change wi
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also reduces chemical noise, which
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Table 1. Extraction Yields, Liquid
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scanning of AMPP amides of the anal
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Anal. Chem. 2010, 82, 6797-6806 δ
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Figure 1. Schematic view of the pre
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from the specimen and enclosed in a
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Figure 4. (A) IRMS mass-44 chromato
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Table 3. Ambient Measurement Result
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Anal. Chem. 2010, 82, 6807-6813 Dir
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Polymerase (1 U per sample). Reacti
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of which was constant for all ampli
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analytically useful signals at less
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carbon black and RP-C18 for the ext
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solution in an equal volume, and 1
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Table 2. Concentrations and Ratios
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Anal. Chem. 2010, 82, 6821-6829 Mac
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mg/mL protein, followed by separati
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Figure 2. Productivity of SEQUST an
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Figure 4. High-resolution MS/MS spe
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Figure 6. Characterization of PSMs
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containing T-T mismatches. 23 Based
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Figure 1. Extinction spectra of sol
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Figure 3. (A) The value of Ex650 nm
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Figure 5. Extinction spectra and co
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wished to explore the dehydration o
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constant medium for separation; we
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Figure 2. Standards of (Pi)n, n ) 1
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Figure 5. Quantitative calibration
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Anal. Chem. 2010, 82, 6847-6853 Met
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Figure 1. 226 Ra spectrum by liquid
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Table 1. Counting Properties and De
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Table 3. Analysis of 226 Ra in Sedi
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mercial microarray scanner and fabr
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Figure 2. Optical transmission meas
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Figure 5. Volcano plots detailing t
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data as well. The 41 genes in Table
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corresponding compound if its chemi
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Figure 1. Schematic illustration of
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Table 1. Absolute Quantification Re
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ment. Therefore, the long-time drea
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Figure 1. Chemically actuated micro
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Figure 2. Influence of a surfactant
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Figure 5. Device to eject and mix s
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Anal. Chem. 2010, 82, 6877-6886 Imm
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NaCl, phosphate buffer saline (PBS)
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Figure 2. Product ion mass spectra
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-70 °C resolved the problem, givin
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Table 1. Intraday Precision and Acc
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Anal. Chem. 2010, 82, 6887-6894 Fer
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Figure 1. Infrared spectra of (A) u
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Figure 3. Cyclic voltammograms obta
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Figure 6. Calculated charge from ch
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Anal. Chem. 2010, 82, 6895-6903 Ele
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Table 1. Chemical Structure, pKa Va
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pH with a tilted baseline (Figure 1
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Table 2. Linearity and Detection Li
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Page 161 and 162: the multielement capabilities, the
Page 163 and 164: RESULTS AND DISCUSSION Sulfur Detec
Page 165 and 166: Table 2. Molecular Properties and C
Page 167 and 168: Anal. Chem. 2010, 82, 6911-6918 Dir
Page 169 and 170: dilution and hybridization buffer.
Page 171 and 172: solution under appropriate incubati
Page 173 and 174: Figure 4. Standardization curve for
Page 175 and 176: Anal. Chem. 2010, 82, 6919-6925 Ele
Page 177 and 178: the ×10 objective, to have a large
Page 179 and 180: Figure 2. With a suitable removal o
Page 181 and 182: the NB signal in a much better foot
Page 183 and 184: Scheme 1. Reactions of Selenium Rea
Page 185 and 186: Figure 2. (a) ESI-MS spectrum showi
Page 187 and 188: Figure 4. (a) ESI-MS spectrum showi
Page 189 and 190: Anal. Chem. 2010, 82, 6933-6939 Dif
Page 191 and 192: trode 28 by a finite element using
Page 193 and 194: Figure 4. Comparison between simula
Page 195 and 196: Figure 6. Comparison between simula
Page 197 and 198: educed in the vicinity of double bo
Page 199 and 200: Figure 2. Normalized product ion ab
Page 201 and 202: Figure 4. EID (a) and IRMPD (b) of
Page 203 and 204: Anal. Chem. 2010, 82, 6947-6957 Ide
Page 205 and 206: Figure 1. Schematic flowchart showi
Page 207 and 208: difference, ppm compound Table 1. I
Page 209: isoforms, its successful use, in th
Page 213 and 214: m/z 1172.935 was observed for Ser14
Page 215 and 216: linked products via affinity tags.
Page 217 and 218: Scheme 2. Fragmentation Mechanism o
Page 219 and 220: Figure 1. (A) ESI-LTQ-CID-MS 2 prod
Page 221 and 222: Figure 3. (A) ESI-LTQ-CID-MS 2 prod
Page 223 and 224: Figure 5. (A) MALDI-TOF/TOF product
Page 225 and 226: Anal. Chem. 2010, 82, 6969-6975 Ana
Page 227 and 228: Figure 3. Equilibrium response as a
Page 229 and 230: Figure 6. The average measured resp
Page 231 and 232: for the fill time, and we find that
Page 233 and 234: nucleotide tails. 3-5 Thus, the amo
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Page 237 and 238: quent ligation of the aptamers afte
Page 239 and 240: Anal. Chem. 2010, 82, 6983-6990 Imp
Page 241 and 242: Figure 2. Configuration editor wind
Page 243 and 244: Figure 4. Dependencies between volu
Page 245 and 246: Since the time for liquid expulsion
Page 247 and 248: Anal. Chem. 2010, 82, 6991-6999 Int
Page 249 and 250: Figure 1. Representation of the Car
Page 251 and 252: Table 2. Capillary Electrophoresis
Page 253 and 254: Figure 4. (A) Typical raw electroph
Page 255 and 256: field. DNA profiles were delivered
Page 257 and 258: Another approach to handle this lim
Page 259 and 260: (principal components, PCs) in whic
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Figure 5. Relevant score plots and
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CONCLUSIONS In this paper we have i
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electroactive-species loaded liposo
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Figure 2. Qdot-based FLFTS response
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Figure 6. Fluorescence imaging of Q
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Anal. Chem. 2010, 82, 7015-7020 Sel
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Table 1. Effect of 1 D-LC Condition
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Figure 3. Peak-production rate vers
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Anal. Chem. 2010, 82, 7021-7026 Cel
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luciferase (T7 control vector) was
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Figure 3. Inhibitory effects of luc
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Anal. Chem. 2010, 82, 7027-7034 Pat
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Electrochemistry. Electrochemical m
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Figure 2. SEM images of Au substrat
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Table 3. Film Thickness Measurement
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Anal. Chem. 2010, 82, 7035-7043 Qua
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Figure 1. (A) FL spectra of BSPOTPE
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Figure 4. (A) Variation in the FL i
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Figure 6. (A) FL spectra of BSPOTPE
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Scheme 1. Proposed Mechanism for Fl
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one or more drawbacks including poo
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Figure 2. Free fluorophores do not
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Anal. Chem. 2010, 82, 7049-7052 Dev
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Figure 2. Comparative analysis of d
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Analytical Chemistry Chemical Cytometry Quantitates Superoxide

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