omation mbers - Society for Laboratory Automation and Screening

4:00 pm Tuesday, February 3 Proteomics – Arrays Room B1
Zheng Ouyang
Purdue University
560 Oval Drive
West Lafayette, Indiana 47906
ouyang@purdue.edu
Protein Array Preparation by Ion Soft-Landing
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Co-Author(s)
Z. Takats, T. M. Blake,
B. Gologan, J. M. Wiseman,
J. C. Oliver, V. J. Davisson,
R. G. Cooks
A new method has been developed for preparing microarrays of biomolecules via soft-landing of mass selected,
multiply-changed ions. A modified single quadrupole mass filter and a custom-built linear ion trap mass
spectrometer have been used to select the ions and deposit them onto surfaces including: polycrystalline gold,
functionalized self-assembled monolayers, and liquid thin films, at landing energies of 10-20 eV. The effectiveness
of this method has been demonstrated via the preparation of a four-component array of proteins from a mixture
of cytochrome C, apomyoglobin, lysozyme and insulin. The landed materials were recovered and analyzed by
electrospray and MALDI mass spectrometry as well as being characterized in situ by laser desorption. Biological
activity of the landed species was confirmed in the case of lysozyme, and in other experiments in the cases of
trypsin, hexokinase and protein kinase A. The landing efficiencies were estimated to be a few tens of % and
the spot sizes are ca. 1 mm in radius. The conditions for the soft-landing of proteins and peptides have been
optimized. Neutralization of the ions on the surfaces was studied and both complete and incomplete neutralization
were found, depending on the properties of the surfaces. The applications of this method to biology study and
drug discovery as well as the modification of commercial mass spectrometers for soft-landing are discussed.
4:30 pm Tuesday, February 3 Proteomics – Arrays Room B1
Peter Wagner
Zyomyx, Inc.
26101 Research Road
Hayward, California 94545
pwagner@zyomyx.com
New Detection Principles for Protein Biochips
Protein biochips are becoming increasingly important for extracting comprehensive biological information
from smallest sample volumes. Applications include the quantification of gene expression at the protein level,
the measurement of protein-protein interactions, as well as functional protein activity. The diversity in protein
measurements and assay variations requires different types of chip architectures and detection modes. Zyomyx is
uniquely positioned to address the problems associated with micro-scale protein detection and characterization.
Using proprietary biochip-based tools, Zyomyx has developed several novel platforms for proteomic research. An
overview of protein biochip technologies will be presented with an emphasis on label-independent detection.
PODIUM ABSTRACTS