omation mbers - Society for Laboratory Automation and Screening

2:30 pm Thursday, February 5 Microfluidics – Small Volume Dispensing Room A4
Terry D. Lee
Beckman Research Institute of the City of Hope
1500 E. Duarte Road
Duarte, California 91010
tdlee@coh.org
An Electrochemical Pumping System for On-Chip Gradient Generation
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Co-Author(s)
Yunan Miao
Beckman Research Institute of the City of Hope
Jun Xie, Jason Shih, Qing He, Yu-Chong Tai
California Institute of Technology
Electrochemical actuation is unique in its ability to generate large displacements at high pressure while operating
at a very low voltages. It is a viable alternative to the electrokinetic based approaches that are used in most
microfluidic applications. We have developed a microscale, chip-based pumping system that is capable of
delivering fluids over a range of flow rates (20–1000 nL/min) and pressures (0–200 psi). The devices described
in this work were fabricated on the surface of a silicon wafer using a batch process that lends itself to mass
production. The electrochemical pumps were integrated with other micromachined components on the chip
including a low volume mixing chamber, a packed reverse phase column, and an electrospray ionization source.
The work that will be presented represents significant progress toward a complete HPLC system on a chip.
3:00 pm Thursday, February 5 Microfluidics – Small Volume Dispensing Room A4
Thomas Corso
Advion BioSciences, Inc.
15 Catherwood Road
Ithaca, New York 14850
boardmaa@advion.com
Fully Automated Nanoelectrospray With a BioMEMS Device
Co-Author(s)
Sheng Zhang
Colleen Van Pelt
Analytical sciences are moving toward miniaturization of chemical analysis systems. BioMEMS or Lab-on-a-chip
systems offer advantages such as increased sample throughput, improved reproducibility, higher sensitivity, and
significantly lower cost per sample over conventional, higher flow rate analyses. Although numerous microfluidic
devices have been reported, applications rely primarily on spectroscopic detection in part due to the lack of a
viable interface between microchip-based technology and mass spectrometry. Mass spectrometry is a powerful
research tool for protein identification and more recently noncovalent interactions, however, current methods are
labor intensive and low throughput. This presentation describes the first automated nanoelectrospray system for
mass spectrometry, the NanoMate 100, allowing for automated analysis with low sample consumption. The
technology is based on a fully monolithic microchip device, ESI Chip, containing an array of 10 x 10 single-use
nanoelectrospray emitters. The microchip is fabricated from silicon substrates using deep reactive ion etching
and other semiconductor techniques. The system eliminates carryover as each sample has its own disposable
delivery tip and nanoelectrospray nozzle. Applications including protein-ligand noncovalent interaction studies and
applications using on-line separation technology will be discussed.
PODIUM ABSTRACTS