omation mbers - Society for Laboratory Automation and Screening

11:00 am Thursday, February 5 Microfluidics – Bioanalytical Room A4
Adrian Winoto
Caliper Technologies Corp.
605 Fairchild Drive
Mountain View, California 94043
adrian.winoto@calipertech.com
79
Co-Author(s)
Sherri Biondi, Andrea Chow,
Bahram Fathollahi, Jim Mikkelsen,
Michael Spaid, Ravi Vijayendren
Protein Sizing and Relative Quantitation Determination Using a Microfluidic LabChip® Device
SDS-Page has been the predominant protein sizing method for the past 30 years. This technique involves multiple
manual operations including separation, staining, destaining and detection and typically requires several hours.
We have developed a high throughput protein sizing assay which integrates each of these operations into a
single microfluidic LabChip ® device. An assay is run by sipping unlabeled protein samples into the device using
vacuum. The samples are then electrokinetically loaded and injected into the separation column which contains
a low viscosity polymer sieving matrix. Both protein-SDS complexes and free SDS micelles are fluorescently
stained during the separation process. Prior to detection, the sample is diluted to reduce the SDS concentration
below its critical micelle concentration. This destaining step effectively reduces the background fluorescence
from micelle-dye complexes so that protein-SDS-dye complexes can be detected. Using this technique we are
able to size proteins between 14 and 200 kDa. The microfluidic device delivers a throughput of 75 seconds/
sample with unattended sample sipping from a 96-well plate. In this presentation, we will show the experimental
data describing the fundamental work to understand the assay performance. We will also describe the assay
reproducibility in terms of sizing and mass quantitation. Finally, we will demonstrate assay performance with a
broad range of customer sample types.
11:30 am Thursday, February 5 Microfluidics – Bioanalytical Room A4
Laurie Locascio
National Institute of Standards and Technology
100 Bureau Drive
Gaithersburg, Maryland 20899-8394
laurie.locascio@nist.gov
Using Liposomes for High-Efficiency Mixing in Microfluidic Systems
Co-Author(s)
Wyatt Vreeland
Andreas Jahn
Michael Gaitan
Liposomes have been used for many years in a variety of clinical and pharmaceutical applications related to
drug encapsulation, targeting, and delivery. Currently, we are exploring analytical uses of liposomes focusing on
their application in microfluidic systems as selective reagents for the performance of automated and targeted
microchemical reactions. In this work, reagents are encapsulated inside the aqueous interior of liposomes
that are dispersed in solution in a microfluidic channel. Reagent release is triggered through the modulation of
temperature using an external heat source to locally change the solution temperature in the channel. The reagent
release temperature is tunable based on liposome formulation; therefore, liposomes with different reagents can
be programmed to sequentially release their contents thus enabling exquisite control of reaction timing. Because
liposomes are evenly dispersed in the microchannel, reagent mixing in the microfluidic environment is very
rapid upon release. We have also recently been exploring methods for the automated formation of liposomes in
microfluidic systems. In this presentation, we will discuss several aspects of our research related to liposomes in
microfluidic systems including the formation of liposome vesicles in microfluidic systems under various conditions;
encapsulation efficiencies of different reagents inside liposomes made in microfluidic systems; and their application
as reagents for automated microfluidic chemical reaction.
PODIUM ABSTRACTS