omation mbers - Society for Laboratory Automation and Screening
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omation mbers - Society for Laboratory Automation and Screening

omation mbers - Society for Laboratory Automation and Screening omation mbers - Society for Laboratory Automation and Screening

slas.org
13.01.2013 Views

9:30 am Wednesday, February 4 High Throughput Screening – Informatics Room A2 Martin Daffertshofer Evotec Technologies GmbH Schnackenburgallee 114 Hamburg, 22525 Germany martin.daffertshofer@evotec.technologies.com Information Technology for High Content Screening of Miniaturized Cellular Assays 62 Co-Author(s) Paavo Helde Olavi Ollikainen The need for miniaturised cellular assays has grown in the pharmaceutical industry and so does the need for high throughput and high content screening platforms of living cells. In this talk we will present experiences in cellular µHTS using the EVOscreen Mark III platform including the high content confocal imaging system Opera. IT challenges for the analysis and data management of 200000 cell images in 2h hours will be discussed in detail. 10:30 am Wednesday, February 4 High Throughput Screening – Analytical Room A2 Jörg Stappert Greiner Bio-One Maybachstrasse 2 Frickenhausen, 72636 Germany Joerg.stappert@gbo.com HTAPlate: Unique 96-Well Plate for DNA- and Protein Arrays Co-Author(s) Heinrich Jehle, Günther Knebel Greiner Bio-One Florian Winner Lambda GmbH DNA- and protein microarrays are a proven tool in academic research, but several obstacles have to overcome before entering the diagnostic market. Glass slides, the ‘classical’ platform for microarrays, are an inappropriate platform as sample numbers are very restricted, the dimensions are not robot friendly and the robustness for clinical laboratories is missing. In order to overcome these major drawbacks, GBO has developed the first versatile 96-well microplate, designated HTAPlate (High Throughput Array), which has been exclusively designed for DNA- or protein arrays. This HTAPlate overcomes all restrictions associated with standard 96-well microplates, as special features have been incorporated, e.g., low rim wells for spotting on the fly, easy access to the wells for top and bottom reading, volume adjustment for washing and hybridization. We present data proving the robustness of this new platform using our commercial available Periodontitis test (ParoCheck).

11:00 am Wednesday, February 4 High Throughput Screening – Analytical Room A2 Paren Patel Nanostream 580 Sierra Madre Villa Pasadena, California 91107 paren.patel@nanostream.com Accelerating Discovery Analytical Chemistry Through Micro Parallel Liquid Chromatography The Nanostream Veloce system – which includes an instrument, software, and replaceable microfluidic cartridges – incorporates pressure-driven flow to achieve chromatograms comparable to conventional HPLC instrumentation while offering a dramatic increase in sample analysis capacity. The system enables parallel chromatographic separations and simultaneous, real-time UV detection for rapid access to analytical results. Each Nanostream Brio cartridge, made of polymeric materials, incorporates twenty-four columns packed with various stationary phase materials to achieve reverse phase separations. Mixing and distribution of the mobile phase to each of the 24 columns is precisely controlled in each cartridge. This presentation demonstrates specific benefits of the Veloce system for a range of applications, from compound library management to protein digests to physicochemical property testing in ADMET. Future directions will also be discussed. 11:30 am Wednesday, February 4 High Throughput Screening – Analytical Room A2 Richard Ellson Picoliter, Inc. 1190 Borregas Sunnyvale, California 94089 ellson@picoliterinc.com 63 Co-Author(s) Mitchell Mutz, Clifford Brown, Brent Browning, Richard Stearns, David Harris, Ma Phiengsai, Roeland Papen DMSO Hydration Monitoring by Ultrasound in Compound Library Microplates and Implications for Tracking Compound Dilution Dimethyl sulfoxide (DMSO), the most common solvent for storage of compound libraries in microplates, is highly hygroscopic. When exposed to the humidity of a normal laboratory atmosphere, the DMSO in each well absorbs water and thus dilutes the concentration of its compound. Ultrasonics provides a method for measuring the hydration level of DMSO in microplates with CV’s under 2% by detecting reflected ultrasonic waves from the bottom of microplate wells. No removal of test samples, addition of any chemical marker, or storage in clear or unsealed microplates is required. Results from a time-course study of hydration of a 384-well plate exposed to a humid laboratory atmosphere will be shown. By tracking hydration of DMSO in microplates, real-time predictions of library compound concentrations can be made. Implications for use of hydration-corrected compound concentration in better quantifying compound transfer and improving data quality in IC50 experiments will be discussed. PODIUM ABSTRACTS

9:30 am Wednesday, February 4 High Throughput <strong>Screening</strong> – In<strong>for</strong>matics Room A2<br />

Martin Daffertshofer<br />

Evotec Technologies GmbH<br />

Schnackenburgallee 114<br />

Hamburg, 22525 Germany<br />

martin.daffertshofer@evotec.technologies.com<br />

In<strong>for</strong>mation Technology <strong>for</strong> High Content <strong>Screening</strong> of Miniaturized Cellular Assays<br />

62<br />

Co-Author(s)<br />

Paavo Helde<br />

Olavi Ollikainen<br />

The need <strong>for</strong> miniaturised cellular assays has grown in the pharmaceutical industry <strong>and</strong> so does the need <strong>for</strong><br />

high throughput <strong>and</strong> high content screening plat<strong>for</strong>ms of living cells. In this talk we will present experiences in<br />

cellular µHTS using the EVOscreen Mark III plat<strong>for</strong>m including the high content confocal imaging system Opera. IT<br />

challenges <strong>for</strong> the analysis <strong>and</strong> data management of 200000 cell images in 2h hours will be discussed in detail.<br />

10:30 am Wednesday, February 4 High Throughput <strong>Screening</strong> – Analytical Room A2<br />

Jörg Stappert<br />

Greiner Bio-One<br />

Maybachstrasse 2<br />

Frickenhausen, 72636 Germany<br />

Joerg.stappert@gbo.com<br />

HTAPlate: Unique 96-Well Plate <strong>for</strong> DNA- <strong>and</strong> Protein Arrays<br />

Co-Author(s)<br />

Heinrich Jehle, Günther Knebel<br />

Greiner Bio-One<br />

Florian Winner<br />

Lambda GmbH<br />

DNA- <strong>and</strong> protein microarrays are a proven tool in academic research, but several obstacles have to overcome<br />

be<strong>for</strong>e entering the diagnostic market. Glass slides, the ‘classical’ plat<strong>for</strong>m <strong>for</strong> microarrays, are an inappropriate<br />

plat<strong>for</strong>m as sample nu<strong>mbers</strong> are very restricted, the dimensions are not robot friendly <strong>and</strong> the robustness <strong>for</strong><br />

clinical laboratories is missing. In order to overcome these major drawbacks, GBO has developed the first versatile<br />

96-well microplate, designated HTAPlate (High Throughput Array), which has been exclusively designed <strong>for</strong> DNA-<br />

or protein arrays. This HTAPlate overcomes all restrictions associated with st<strong>and</strong>ard 96-well microplates, as special<br />

features have been incorporated, e.g., low rim wells <strong>for</strong> spotting on the fly, easy access to the wells <strong>for</strong> top <strong>and</strong><br />

bottom reading, volume adjustment <strong>for</strong> washing <strong>and</strong> hybridization. We present data proving the robustness of this<br />

new plat<strong>for</strong>m using our commercial available Periodontitis test (ParoCheck).

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