omation mbers - Society for Laboratory Automation and Screening

9:00 am Wednesday, February 4 HT Chemistry – Analytical Room B3
Dave Rakestraw
Eksigent Technologies
2021 Las Positas Court, Suite 161
Livermore, California 94550
djrakestraw@eksigent.com
Rapid, High Resolution Multiplexed HPLC System
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Co-Author(s)
Doug Cyr, Roger Farrow,
Karen Hahnenberger, Don Arnold,
David Neyer, Jason Rehm,
Ken Hencken, Philip Paul
The use of HPLC for high throughput analysis has been limited by the time required for chromatographic
separations and the number of individual instruments that can be dedicated to the application. We will present
data from a new high performance liquid chromatography platform capable of performing ~10,000 separations
per day. This high throughput has been made possible by extremely rapid gradient delivery that dramatically
reduces traditional separation times and the development of a single instrument capable of performing 8 parallel
separations. Low delay volumes enable the use of rapid gradients with durations as low as 10 seconds. The
rapid gradients are achieved using Eksigent’s Microfluidic Flow Control (MFC) system, and result in retention time
repeatability of