omation mbers - Society for Laboratory Automation and Screening
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omation mbers - Society for Laboratory Automation and Screening

omation mbers - Society for Laboratory Automation and Screening omation mbers - Society for Laboratory Automation and Screening

slas.org
13.01.2013 Views

WP086 Ruth Zhang Beckman Coulter, Inc. 7451 Winton Drive Indianapolis, Indiana 46268 ruth.zhang@sagian.com Plasmid Purification Using Promega’s Wizard* SV96 Reagents and Beckman Coulter’s Biomek ® 3000 Laboratory Automation Workstation 234 Co-Author(s) Chad Pittman Scott Boyer Laura Pajak The information included in this poster describes the utilization of a new liquid handler, the Biomek 3000 Laboratory Automation Workstation, in the preparation of plasmid DNA using Promega’s Wizard SV 96 reagents. Using this system, plasmids are purified via binding to and eventual elution from a silica matrix using vacuum filtration. The following system components for the purification of plasmids will be described: • The new automated workstation, the Biomek 3000 • The software and method to drive the workstation • The results when purifying plasmids using Wizard SV96 reagents. Representative data obtained from the purification of plasmids using this system will be shown. Data from automated capillary sequencing on Beckman Coulter’s CEQ 8000 Genetic Analysis System will be shown demonstrating the suitability of the purified plasmids for stringent assays such as capillary sequencing. WP087 Juergen Zimmermann EMBL Genomics Core Facility Meyerhofstrasse 1 69117 Heidelberg D69117 Germany zimmermann@embl-heidelberg.de Co-Author(s) Vladimir Benes, Christian Boulin, and Ralf Griebel Paul Lomax, Perkin Elmer Life Sciences Thomas Zinn, Ullrich Schübel, Macherey Nagel, and Klaus Günther Eberle, Hettich GmbH & Co KG Return of the Centrifuge: Automated DNA Extraction by Small Production Islands General liquid handling systems are well established and centrifuges are judged sometimes even as old fashioned in the field of automation. Nevertheless the combination of both systems is not wide spread in smaller systems. The combination of continuous and discontinuous processes is compromising either throughput or load balancing. Additional hardware is necessary for the interface of both instruments. Purification of DNA by Silica based methods is also a well established procedure which is automated nowadays with vacuum driven robotic systems. This automation approach is problematic under several circumstances. Especially sets of complex sample material (e.g., animal tissues, plant tissues, forensic material) may overload conventional instruments. Variations in the viscosity of a single sample may ruin the output of a complete run, as the flow through is not guaranteed. The presented automated solution combines the stability of silica based purification chemistry and automated centrifugation, resulting in higher reproducibility and offering access to samples which couldn’t be processed so far.

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