omation mbers - Society for Laboratory Automation and Screening

WP084
Michael Yavilevich
Inventor
5 Haim Street Apt. 4
Kiriat Bialik27076 Israel
fastspin@barak-online.net
Fast Spin Centrifuge, Analytical Module and Automated Laboratory System
The present project relates to Rapid Centrifuge and compact Analytical Module like elements of Fast Automated
Laboratory System. Fast Spin Technology conducts specimen separating and cap removing during same spin
or use only cap removing step for previously separated tubes. Some new methods and devices were invented
developing said Fast Spin Centrifuge and compact Analytical Module. Fast Spin realizes variable inclination spin
method. This allows more rapid phase separation between serum, clot and gel. In a second stage the tubes
spin while their longitudinal axes are aligned with the direction of the centrifugation force to allow reliable gel
seal. Separation process in variable inclination tubes was investigated. This study allows at first to develop rapid
Centrifuge, to continue in developing Pre-Analytical Station and complete in Analytical Module which aimed to
solve the problem of modular automation of pre-analytical and analytical clinical laboratory processes. Fast Spin
Technology is best suited to LaboratoryAutomation. Fast Spin is not only rapid separator. It is a powerful preanalytical
and analytical station. This Technologysimplifies connecting tubes with Analyzer. Prototypes of the Rapid
Centrifuge and cap removing device were built and successfully tested in some local laboratory. Preliminary results
confirm the feasibility of the project. Invented new Analytical Module use Batch Technology for loading-unloading
tubes, removal-replacement a caps, rapid centrifugation and specimen testing. This Technology intends to improve
structure and productivity of all Clinical Laboratory Automated System. Fast service will bring new clients to these
Labs. Fast Spin contains original technical and technological ideas and is in the global demand.
WP085
Lilly Zhang
Amgen, Inc.
Pharmacokinetics and Drug Metabolism
One Amgen Center Drive MS 1-1B
Thousand Oaks, California 91320
leiz@amgen.com
Quantitative Biological Sample Analysis Using NanoESI (Nanomate 100 ® ) –MS/MS
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Co-Author(s)
John D. Laycock
Krys J. Miller
The Nanomate 100 ® evaluation for quantitative analysis of exploratory pk screening is discussed. Calibration
curves of Amgen compound A in plasma were analyzed on both Nanomate 100 ® –MS/MS and conventional
LC-MS/MS. API3000 mass spectrometer was used in both cases. Results were compared on curve fitting, LLOQ,
dynamic range, accuracy, precision, carryover, and matrix effect. The results show that Nanomate 100 ® performs
comparably to LC-MS/MS. Nanomate 100 ® also exhibits advantages such as no cross contamination, minimal
sample consumption, and much shorter sample cycle time. In addition, quantitation results of real study samples
are shown. More than 100 plasma samples from various studies were analyzed on Nanomate 100 ® –MS/MS in
parallel with LC-MS/MS. The results showed good correlation between the two interfaces. However, in many cases
Nanomate ESI chip requires further sample clean up, and it can often be labor intensive. Additional advantages
and limitations are also discussed. Overall, Nanomate 100 ® currently does not offer significant advantage in
quantitative analysis of multiple analytes at sub-nanogram levels in early in vivo exploratory pk screening due to
matrix suppression. However, Nanomate 100 ® demonstrates great potentials in multiple research areas such as
Caco-2 screening, microsomal inhibition, and metabolite ID.
POSTER ABSTRACTS