omation mbers - Society for Laboratory Automation and Screening

WP072
Surekha Vajjhala
Nanostream, Inc.
580 Sierra Madre Villa Avenue
Pasadena, California 91107
surekha.vajjhala@nanostream.com
Rapid Compound Purity Screening using the Nanostream Veloce System
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Co-Author(s)
Li Zhang
Paren Patel
Sergey Osechinskiy
Over the past decade, advances in combinatorial chemistry and target characterization have significantly increased
the number of drug candidates and targets available to drug discovery screeners. To ensure a meaningful screen,
an increasing number of pharmaceutical companies are now evaluating compound purity. The Nanostream Veloce
system, together with 24-column Brio cartridges, offers a novel approach to micro parallel liquid chromatography.
This system allows users to achieve unprecedented throughput for standard assays while matching the
performance of conventional LC instrumentation, thus enabling a cost effective way to routinely monitor compound
purity with minimal modification to current methods and work flow. This poster presents results of a study of
pharmaceutical compound library samples using the Veloce system. Individual chromatograms, percent purity
results, study duration and total solvent consumption are compared to results obtained using conventional HPLC.
WP073
Scott Van Arsdell
Pierce Biotechnology, Inc.
Research and Development
30 Commerce Way
Woburn, Massachusetts 01801
svanarsdell@perbio.com
SearchLight Proteome Arrays: Multiplexed Assays for High Content Screening
Co-Author(s)
Rajiv Pande
Christine Burns
A SearchLight Proteome Array for quantitative detection of proteins is described. The array is created by
spotting 25 capture antibodies in a 5 X 5 pattern at the bottom of each well in a 96-well polystyrene microtiter
plate. Target proteins are ‘captured’ by appropriate arrayed antibodies upon sample introduction. Biotinylated
secondary antibodies are added and specifically bind the captured protein. Streptavidin-horseradish peroxidase
conjugate is subsequently added to tag the ‘biotinylated antibody – protein – capture antibody’ sandwich, followed
by the addition of a chemiluminescent substrate. The plate is imaged with the SearchLight CCD Imaging and
Analysis System, and the density of each spot is quantified. A cocktail of recombinant target proteins is assayed
on the plate to generate standard curves and allow quantification of analytes in the samples. We describe the use
of a SearchLight 25–plex array to quantitate a panel of cytokines and chemokines (human IL-1 alpha, IL-2, IL-4,
IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-18, IFN-gamma, TNF-alpha, RANTES, Eotaxin, MDC, TARC, I-309, MIP-1
alpha, MIP-1 beta, MCP-1, GRO-alpha, NAP-2, IP-10, MIP-3 alpha, and MIP-3 beta). The array was utilized to
monitor cytokine and chemokine expression in mitogen stimulated peripheral blood mononuclear cells (PBMCs).
We demonstrate the applicability of SearchLight Proteome Arrays for high content screening by simultaneously
quantifying 25 analytes per well (2400 data points in a 96-well plate, up to 80 samples per plate). SearchLight
assays are simple, very sensitive and rapid (~2hrs); and the technology is compatible with plate based robotics.
POSTER ABSTRACTS