omation mbers - Society for Laboratory Automation and Screening

WP056
Steven Sheridan
Millipore
Life Sciences
17 Cherry Hill Drive
Danvers, Massachusetts 01923
steven_sheridan@millipore
219
Co-Author(s)
Sonia Gil
Libbey Kellard
Scaling From 96- to 384-Well Assay Platforms: Characterization of Receptor-Ligand Binding
Using 384-Well Filter Plates Optimized For Maximum Radiation Detection
A significant portion of the drug discovery process involves the systematic screening of large compound libraries
for specific binding to various cellular receptors. For decades, heterogeneous filter binding assays have been
used for this purpose, particularly under more challenging conditions such as when the receptor is prepared
from unpurified tissue homogenates or cell membrane fragments. To date, most screening has been performed
on 96-well platforms. However, with a rapidly increasing number of compounds available from natural product
isolation and combinatorial chemistry, a bottleneck has been created which has made it necessary to develop
higher throughput platforms that make it possible to perform adequate library screening in a timely and cost
effective manner. A 384-well filter plate has been developed and optimized for automated radiometric receptorligand
binding assays. This 384-well filter binding format increases throughput of receptor-ligand binding screening
without sacrificing sensitivity, robustness or precision. The design is optimized for maximum radiation detection
and is compatible with coincidence scintillation counting making it possible to achieve the same low-end sensitivity
as attained with a 96-well plate. Using parallel experimental procedures, reagents and receptor systems, data are
presented that demonstrate the scalability of radiometric receptor-ligand binding assays from a 96- to a 384-well
format. The higher density of the 384-well filter plate allows for quantitative receptor-ligand binding assays in a
robust, fast, and reagent saving format.
WP057
Christopher Silva
Molecular Devices Corporation
1311 Orleans Drive
Sunnyvale, California 94089
chris_silva@moldev.com
SpectraMax M2 Multi-detection System Allows Validated Microplate-based Fluorescence and
Absorbance Assays in a GLP/GMP Environment
The SpectraMax M2 multi-detection microplate reader with dual-mode cuvette port allows a wide rage of
fluorescence (FI) and absorbance (Abs) assays to be converted into QC, manufacturing and pre-clinical
environments. Dual monochromators, uv/vis wavelength range, PathCheck, fluorescence, and absorbance
validation plates with integrated SoftMax Pro data analysis and FDA 21 CFR Part 11 compliant tools and an
automation interface make the transfer of assays from research and development to the GLP/GMP environment
straightforward.
POSTER ABSTRACTS