omation mbers - Society for Laboratory Automation and Screening

WP027
Arja Lamberg
Thermo Electron
Microplate Instrumentation
Ratastie 2, P.O. Box 100
Vantaa01620 Finland
arja.lamberg@thermo.com
Co-Author(s)
Merja Mehto and Arja Lamberg, Thermo Electron
Stine Bergholtz, David Gillooly, Diem Tran, Tine Borgen, Dynal Biotech
Virpi Kymäläinen and Maija Partanen, Thermo Labsystems
KingFisher 96 – A Novel High Throughput Platform for Protein Applications
Sample preparation is often a limiting step for proteomic applications and requires solutions for high throughput
template preparation. Magnetic particle technology provides rapid and easy to automate method for sample
preparation from different sources. KingFisher family consists of three magnetic particle processors for customers
having different needs for sample preparation throughput. The patented technology is based on concept where
magnetic particles are transferred instead of liquids. The system is designed to automate the sample preparation
process of proteins, nucleic acids and cells. We have now developed an automated method for the isolation of
recombinant polyhistidine tagged proteins using KingFisher 96 and super-paramagnetic particles Dynabeads ®
TALON. Dynabeads ® TALON particles employ a cobalt-based Immobilized Metal Affinity Chromatography
(IMAC) on which BD TALON chemistry has been immobilized. Compared to technologies that employ nickelbased
IMAC, these cobalt-based beads are able to bind polyhistidine tagged proteins with an enhanced selectivity.
Using this system the isolation of 96 polyhistidine tagged proteins from bacterial lysate with high purity takes less
than 25 minutes. The proteins eluted from, or bound to magnetic particles can be directly used in downstream
applications such as phage display, aptamer screening or other drug discovery applications. In this poster the
excellent performance of KingFisher 96 and Dynabeads ® TALON is pointed out by referring the analysis data of
purified proteins.
WP028
Fred Lange
University of Rostock
Institute of Automation
R.-Wagner-Str. 31
Rostock18119 Germany
fred.lange@uni-rostock.de
205
Co-Author(s)
Regina Stoll
Thomas Renger
High Resolution Mobile Measurement of Oxygen Concentration in Main Breath Stream by
Optical Oxygen Sensing – An Application in Occupational and Sports Medicine
Measurement of cardiocirculatory parameters like heart rate and blood pressure does not bring sufficient results
for evaluation of metabolic components of physical behavior of the organism especially regarding strain under
labour and different intensities of sport’s activities. So, new ways for miniaturizing and optimizing of gas sensors
are important. The development result shows an application of a miniature, high resolution fast optical fluorescence
based measurement system positioned in the breath main stream. In a first step the sensor is inplemented into a
mask, in further developments a “headset ensemble” is the goal.The system can be used for functional and fitness
determination in the sport’s medical diagnostics lab as well as in the field or in labour environments. A special
application is performed to be used as a physiological monitoring system in space.
POSTER ABSTRACTS