omation mbers - Society for Laboratory Automation and Screening

WP007
Morten Egeberg
Dynal Biotech ASA
Molecular Systems
P.O. Box 114 Smestad
Oslo0309 Norway
morten.egeberg@dynalbiotech.com
Magnetic Bead Based High Throughput PCR- and Sequence Cleanup
195
Co-Author(s)
Tommy Rivrud
Erling Finne
Dag Lillehaug
The advancements in high throughput DNA sequencing technologies the last decade has led to an increased
demand for efficient upstream sample preparation. Thorough removal of contaminants such as primers from
the PCR reaction and un-incorporated dyes, excess nucleotides and salts from the DNA sequencing reaction
combined with high yields of isolated DNA are critical to obtain high quality DNA sequences. To meet these
requirements we have developed automated protocols for PCR- and sequence cleanup on Beckman Coulter’s
Biomek FX liquid handling robot. These protocols are based on Dynabeads ® magnetic separation technology. We
use paramagnetic Dynabeads ® to bind directly to PCR- or sequencing products. When the products are bound to
the beads, a simple magnetic separation event takes place prior to washing of the beads to remove contaminants.
The products are then quickly eluted from the beads for downstream handling. Compared to conventional methods
such as ethanol precipitation and gel filtration, our magnetic bead based technology is ideal for implementation
on automated platforms as no centrifugation or vacuum filtration steps are required. Moreover, we show that the
quality of the data and the robustness of the technology meet the high requirements of today’s high throughput
DNA sequencing facilities.
WP008
Boaz Eidelberg
Bayside Motion Group
Business Development
27 Seaview Boulevard
Port Washington, New York 11050
beidelberg@baysidemotion.com
Co-Author(s)
Joe Timpone
Guidlines for Selecting XYZ Machines for High Precision High Throughput Lab Automation
The future of high quality life longevity lies in biotechnology advances and Lab Automation. The immense amount
of costly testing, needed to identify feasible target molecules, requires efficient Automation tools. Automation
is required for low precision handling of microplates, bio chips, and wells in and out anciliary Lab equipment,
such as environmental chambers and cenrefuges. It is also required for high speed, high precision fluid handling,
assaying and sensing, in a process of detecting gene expression and protein activity. The objective of this article
is to discuss useful guidelines and tools for selecting optimal XYZ machines for various applications in Lab
Automations. Basic definitions of key process performance variables, such as accuracy, repeatability, dead band,
and resolution will be made. Variables effecting process throughput such as jerk, acceleration, velocity, and settling
time will be discussed. In addition, important machine variables effecting image clarity and quality of sensing, such
as constant velocity and jitter will be explained. The XYZ Lab automation machine, is constructed as an assembly
of slides, bases, bearing, encoders, motors, amplifiers, and controllers. Each element possesses numerous
characteristic that effect the overall system performance. The article will highlight the relationship between key
machine component parameters and the desired process performance.An analytical tool, BIMO, which assist in
the selection process of an XYZ machine for Lab Automation process, by analyzing cost/performance tradeoffs
of various options will be discussed. Finally, examples of simulated BIMO runs and actual tested data for various
Lab Automation equipment will be presented. Bayside’s proprietary analysis tool – BIMO, will be distributed to
participating audiance with interest in high precision Automation tools.
POSTER ABSTRACTS