omation mbers - Society for Laboratory Automation and Screening

TP082
Surekha Vajjhala
Nanostream, Inc.
580 Sierra Madre Villa Avenue
Pasadena, California 91107
surekha.vajjhala@nanostream.com
Micro Parallel Liquid Chromatography for High Throughput ADMET Profiling
187
Co-Author(s)
Li Zhang, Paren Patel, Jeffrey Koehler,
Steve Hobbs, Chris Phillips
The Nanostream Veloce system – which includes an instrument, software, and replaceable microfluidic cartridges –
incorporates pressure-driven flow to achieve chromatograms comparable to conventional HPLC instrumentation
while offering a dramatic increase in sample analysis capacity. The system enables parallel chromatographic
separations and simultaneous, real-time UV detection for rapid determination of ADMET properties. Each
Nanostream Brio cartridge, made of polymeric materials, incorporates twenty-four columns packed with standard
(C-18) stationary phase material to achieve reverse phase separations. Mixing and distribution of the mobile phase
to each of the 24 columns is precisely controlled in each cartridge. The system provides an ideal platform to
accelerate assessment of physicochemical properties (i.e., log P, CHI, etc.) for a large number of compounds.This
poster demonstrates how the Veloce system offers specific benefits for rapid assessment of ADMET parameters.
The 24-fold increase in sample analysis capacity allows standard curve generation and simultaneous analysis
of multiple replicates of samples in a single run. By accelerating access to high quality data, the Veloce system
enables scientists to make decisions about promising leads earlier in the drug discovery process.
TP083
Ian Whitehall
TTP LabTech Ltd
Melbourn Science Park, Cambridge Road
RoystonSG8 6EE United Kingdom
inw@ttplabtech.com
Cell Based Assays in 384- and 1536-Well Formats using MosQuito and the Acumen
Explorer
Co-Author(s)
Paul Wylie
Rob Lewis
Wayne Bowen
There is a growing interest in using whole cell assays in screening. By using a 1536-well format, the quantities
of expensive compounds can be substantially reduced having a major impact on the cost of a screen. There are
currently a number of technologies that dispense cells into 384-well formats, but fewer that are able to dispense
viable cells into a 1536-well plate.In this study, we have used the MosQuito liquid handling system, which uses
disposable pipettes, to dispense cells into 384- and 1536-well plates. We have then used the Acumen Explorer
laser scanning fluorescence microplate cytometer to study proliferation of the cells over several days. We have also
shown that we can determine cell cytotoxicity, as an example of a whole cell assay. The results demonstrate that
MosQuito can successfully dispense viable cells into 384 and 1536 microtitre suitable for detection and assay with
the Acumen Explorer.
POSTER ABSTRACTS