omation mbers - Society for Laboratory Automation and Screening

TP078
Regina Stoll
University of Rostock
Institute of Occupational Medicine
St. Georg Str. 108
Rostock18055 Germany
regina.stoll@med.uni-rostock.de
Fuzzy Based Medical Expert System for Automated Data Interpretation in Occupational
Medicine
185
Co-Author(s)
Mohit Kumar
Norbert Stoll
In medical data processing often there is a problem to formulate algorithms for data interpretation. In occupational
medicine i.e., a number of different parameters are acquired for giving an idea to an expert about the status of
physical fitness. The paper describes an neuro-fuzzy approach as a data mining tool for determination of physical
fitness of a patient on a virtual scale between 0 and 1. The advantage of this procedure is, that there is no need for
an analytical description of the complex functionality of physical fitness. So, the network is trained by original data
and are able to fit in new data points on demand. The data used in this system are respiratzory parameters and
heart rate parameters in combination with body type and body fat measurement data. By this way, the knowledge
of an medical expert is combined with the individual parameter levels. The whole system is an artificial medical
expert system for data interpretation.
TP079
Yu Suen
Beckman Coulter, Inc.
Biological Systems Operation
4300 N. Harbor Boulevard
Fullerton, California 92834
ysuen@beckman.com
Co-Author(s)
Keith Roby, Javorka Gunic
Michael H. Simonian, Graham Threadgill
An Automated, High Throughput Cell-Based Assay System Using the Biomek 3000
Laboratory Automation Workstation and CellProbe HT Caspase 3/7 Whole Cell Assay
Using Beckman Coulter’s CellProbe HT Caspase 3/7 Whole Cell Assay in primary or secondary screening provides
quality information on cellular responses to apoptotic regulators. This 35 µL total volume homogenous assay
measures caspase 3/7 activities in a 384-well format. The high throughput and low sample volume cell-based assay
is achieved using the Biomek 3000 Laboratory Automation Workstation for cell preparation, apoptosis induction and
substrate addition. The Affinity Multi-Mode Reader (Cambridge Research Institute, Boston) is an ultra-sensitive
screening platform that uses a focused laser and CCD camera for fluorescent intensity detection. The caspase
3/7 activity is inducer dose-dependent and responder cell number-dependent. Using this system, a strong signal
and a z´ factor of 0.5 were achieved with a minimal cell number. The dose response of DEVD-CHO inhibition of
caspase 3/7 activities was used to demonstrate the superior sensitivity and specificity of this system. The Biomek
3000 Laboratory Automation Workstation facilitated CellProbe HT Caspase 3/7 Whole Cell Assay implementation,
reduced the chance of contamination and minimized the intensive requirement of sterile skills. The automated
CellProbe assay can be used for high throughput screening of drug candidates for apoptosis regulators.
POSTER ABSTRACTS