omation mbers - Society for Laboratory Automation and Screening

TP072
Burkhard Schaefer
National Institute of Standards and Technology
Analytical Chemistry Division
100 Bureau Drive; Building 227; Room A-159
Gaithersburg, Maryland 20899-8394
burkhard.schaefer@nist.gov
Creating the Analytical Information Markup Language (AnIML)
182
Co-Author(s)
Gary Kramer
Multiple, vendor-specific, proprietary data formats have made it difficult to interchange analytical result data
between instruments and applications. When data are interchanged, metadata are rarely included, often
rendering the datasets useless after the particulars of the experiment have been forgotten. The few existing
data exchange standards are not compliant with modern network-based data interchange technologies. A few
years ago NIST began to develop a markup language for uv/visible spectrometry data based on the Extensible
Markup Language (XML). The result, SpectroML, showed the power of this approach and is now integral to
NIST’s data handling in its optical filter Standard Reference Materials program.ASTM subcommittee E13.15 on
Analytical Data Management was formed in part to develop a general markup language, such as SpectroML, for
all spectroscopic and chromatographic result data. Work has begun on the initial task to develop a core schema
representing the information that is common to all analytical techniques. If a general core can be developed and
adopted, standard applications can be written to utilize this core and accomplish simple functions such as data
visualization, importing data into applications such as spreadsheets, etc. With a simple applet a web browser such
as Microsoft’s Internet Explorer could display any spectral or chromatographic trace. More sophisticated concepts
such as integrating peak areas, leveling baselines, etc. will require more complicated software, but having a single
technique-independent routine to view data, paste the visual image into reports, or import data into other programs
for further processing will be of enormous utility. This presentation will describe the current status of the AnIML
development efforts and point out possible uses in various application domains.
TP073
Sadhana Sharma
The Ohio State University
Davis Heart and Lung Research Institute (326 HLRI)
473 W. 12th Avenue
Columbus, Ohio 43212
sharma.119@osu.edu
Rapid and Sensitive Determination of Cardiovascular Drugs in Mouse Plasma Using
Solid-phase Extraction and RP-HPLC
Co-Author(s)
John Shapiro
Stephen C. Lee
Mauro Ferrari
Fast and sensitive determination of the drugs is essential for pharmaceutical automation and disease management.
Capillary electrophoresis, liquid chromatography–mass spectrometry (LC–MS), immunoassays and high-performance
liquid chromatography (HPLC) are some of the methods used for drug analysis. Of these, the HPLC methods
have been used most frequently because of their simplicity, sensitivity, and selectivity. Drug analysis from blood/
plasma samples requires sample pretreatment before actual HPLC analysis. This can be achieved using solidphase
extraction (SPE), protein precipitation and SPE, liquid–liquid extraction (LLE), solvent extraction followed by
column-switching, or solvent extraction and SPE. Higher number of pretreatment steps decreases efficiency and
increases analysis time. In addition, most of these HPLC methods require relatively large (a maximum of 0.5–1.0 ml)
biological sample and multiple extraction steps. In cases where available sample volumes are in microliters, the
HPLC methods with single LLE are generally not considered sensitive enough due to the presence of endogenous
interferences. Solid phase extraction can be useful for such cases. In the present research effort, a rapid, simple and
sensitive method for the determination of simvastatin in mouse plasma is developed. This method utilizes Waters
OASIS solid-phase extractor and Symmetry C8 reversed phase high-performance liquid chromatography column
for micro-sample analysis and is useful for analysis of the drug released from microfabricated silicon nanoporous
implant in mouse/human.