omation mbers - Society for Laboratory Automation and Screening

TP064
Dominik Poetz
National Institute of Standards and Technology
Analytical Chemistry Division
100 Bureau Drive
Gaithersburg, Maryland 20899
dominik.poetz@nist.gov
Using the Analytical Information Markup Language (AnIML) to Represent LC-Diode-Array
Data
ASTM Subcommittee E13.15 has been formed, in part, to develop a standard markup language for spectroscopic
and chromatographic result data. The Analytical Information Markup Language (AnIML) is being created using the
Extensible Markup Language (XML) and is based on existing markup languages for analytical result data such as
NIST’s SpectroML and Thermo’s GAML as well as data definitions and concepts from existing standards such as
JCAMP-DX, the ASTM’s Andi standards, IUPAC definitions, etc. The initial work has begun to create the AnIML
core, the schema representing the data and metadata common to all analytical spectroscopy and chromatography
techniques. The E13.15 subcommittee has asked NIST to develop an example implementation of AnIML for data
from liquid chromatography with diode array detection. This technique was chosen as a model because it is not
overly complex, as contrasted with mass spectrometry or nuclear magnetic resonance spectroscopy, and because
the datasets are inherently two-dimensional. The implementation will be created around the AnIML core and will
consist of a “technique-specific” layer for LC-diode arrays that is vendor independent, along with enough of a
“vendor-specific” layer to realize the application for at least one existing commercial instrument. The goal is to
create a sufficient AnIML representation for data from a actual analytical technqiue to see what works and what
needs revision, to demonstrate the power of the markup language approach, and to serve as a model for those
writing technique specific layers for other analytical spectroscopy and chromatography domains.
TP065
Ketul C. Popat
Boston University
Department of Biomedical Engineering
44 Cummington Street
Boston, Massachusetts 02215
kpopat@bu.edu
178
Co-Author(s)
Tejal A. Desai and Gopal Mor, Boston University
Craig Grimes, Pennsylvania State University
Poly-Ethylene-Glycol Grafted Non-fouling Nanoporous Alumina Membranes
Membranes currently used for separation of sub-micron particles in biomedical applications are of asymmetric
or anisotropic variety and are made from polymers such as polysulfone or polyacrylonitrile. Several
bioincompatibilities are associated with these polymeric membranes limiting their applications. Aluminum oxide
substrates have been chosen for several reasons, including compatibility with our processing protocols, chemical
and thermal stability, and ease in post-processing surface modification. Several studies have shown this material
to be bioinert. However, the biocompatibility of the membrane material must be further investigated and improved.
With proper surface modification, the protein adsorption can be lowered and the non-fouling characteristics can
be improved. In this study we graft alumina membranes with a biocompatible polymer like poly (ethylene glycol) to
investigate protein adsorption. Albumin and Fibrinogen were selected as model proteins since they are important
components in immunogenic and thrombogenic reactions. X-ray photoelectron spectroscopy (XPS) and atomic
force microscopy (AFM) were used to characterize PEG films on alumina membranes.