omation mbers - Society for Laboratory Automation and Screening
omation mbers - Society for Laboratory Automation and Screening
omation mbers - Society for Laboratory Automation and Screening
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TP062<br />
Laura Pajak<br />
Beckman Coulter, Inc.<br />
Biomedical Research Division<br />
7451 Winton Drive<br />
Indianapolis, Indiana 46268<br />
laura.pajak@sagian.com<br />
Automated Proteomic Applications on Beckman Coulter’s Biomek ® NX <strong>Laboratory</strong><br />
Aut<strong>omation</strong> Workstation<br />
177<br />
Co-Author(s)<br />
Chad Pittman<br />
Scott Boyer<br />
The in<strong>for</strong>mation included in this poster describes the utilization of a new automated liquid h<strong>and</strong>ler, the Biomek<br />
NX <strong>Laboratory</strong> Aut<strong>omation</strong> Workstation, <strong>for</strong> proteomic applications. His-Tag proteins were purified from bacterial<br />
cultures in 96-well plates. Following purification, aliquots from the purified samples were quantitated on the<br />
Biomek NX using a Brad<strong>for</strong>d assay. An integrated gripper on the span pod of the Biomek NX permits the microtiter<br />
plate containing the quantitation samples to be placed into an integrated reader, the AD340 ALP. This allows <strong>for</strong><br />
complete walk-away aut<strong>omation</strong> of protein purification <strong>and</strong> quantitation. The following system components <strong>for</strong> the<br />
proteomic applications will be described:<br />
• The hardware requirements <strong>for</strong> the Biomek NX<br />
• Software <strong>and</strong> methods to drive the aut<strong>omation</strong> workstation.<br />
Results obtained when purifying proteins from both uninduced <strong>and</strong> induced bacterial cultures on the worksurface<br />
of the Biomek NX will be described.<br />
TP063<br />
Chad Pittman<br />
Beckman Coulter, Inc.<br />
Applications<br />
7451 Winton Drive<br />
Indianapolis, Indiana 46268<br />
chad.pittman@sagian.com<br />
Aut<strong>omation</strong> of Immunotech’s IL-8 ELISA Using Beckman Coulter’s Assay WorkStation<br />
Version 1.5<br />
Co-Author(s)<br />
Laura Pajak<br />
Scott Boyer<br />
Beckman Coulter, Inc. has developed <strong>and</strong> optimized an automated method using the Assay WorkStation Version 1.5<br />
to provide a complete, walk away system that automates ELISA assays. The system uses SAMI ® WorkStation<br />
scheduling software to optimize the use of incubations to make processing as efficient as possible. The method<br />
allows processing of up to three families (6 plates) in under five hours with minimal reagent waste. On-deck plate<br />
washing <strong>and</strong> reading are achieved by using an integrated Beckman Coulter MW96 washer <strong>and</strong> AD340 Automated<br />
Labware Positioner, respectively. Consumables are stored in an Automated Storage Module Cytomat Microplate<br />
Hotel HS with Plate Shuttle System <strong>and</strong> brought onto the worksurface by the Biomek ® FX Cytomat Automated<br />
Labware Positioner thereby allowing complete processing without user intervention.The in<strong>for</strong>mation provided here<br />
will:<br />
• describe the automated system used to process the ELISA;<br />
• demonstrate the utility of the Assay Workstation;<br />
• describe the results when processing Immunotech’s IL-8ELISA kit on this system.<br />
POSTER ABSTRACTS
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