omation mbers - Society for Laboratory Automation and Screening

TP056
Joseph Machamer
Molecular Devices
1311 Orleans Drive
Sunnyvale, California 94089-1136
joe_machamer@moldev.com
Luminescence Assays and FDA CFR 21 Part 11 Compliance With the New LMax II384
Microplate Luminometer
Luminescence microplate assays continue to grow in popularity as reagent kit vendors introduce new, highly
sensitive assays. Many of these assays are performed in the laboratories of pharmaceutical and biotechnology
companies where compliance with FDA CFR 21 Part 11 is a requirement. Here we report on the results of
applications run on the recently introduced LMax II 384 microplate luminometer from Molecular Devices in a FDA
CFR 21 Part 11 environment.
TP057
Dieudonne Mair
University of California, Berkeley
Department of Chemical Engineering
727 Lattimer Hall
Berkeley, California 94720
dmair@uclink.berkeley.edu
174
Co-Author(s)
Timothy Stachowiak, Jean Frechet, and Emily Hilder,
University of California, Berkeley
Frantisek Svec, Lawrence Berkeley National Laboratory
Monolithic Modules in Micro Total Analytical Systems Rapidly Fabricated From Plastics
We report on the development of microfluidic devices fabricated from plastics, placing a porous polymer monolith
in the channels, and use of these devices in the field of proteomics. To achieve this, a robust nickel master for
injection molding of the devices has been fabricated using the dry etching, electroplating, and molding (DEEMO)
process. Optical and chemical properties of a series of potential thermoplastic polymers have been screened and
the best candidate for the injection molding of microchips – cyclic olefin copolymer Topas 8007x10 – selected.
Since the surface of this material is highly hydrophobic, surface modifications targeting an increase in hydrophilicity
were explored using model systems. According to the contact angle measurements, photografting the surface
with poly[(ethylene glycol) monomethacrylate] (PEGMA) chains afforded a hydrophilic surface similar to that of
poly(ethylene glycols) and led to a significant reduction in protein adsorption. The preparation of rigid, porous,
methacrylate-based monoliths that resist protein adsorption and functions as a static mixers/chemical reactors
was also studied. Hydrophilic monoliths were prepared by adding 2-hydroxyethyl methacrylate (HEMA) to the
polymerization mixture. The pore size, measured by mercury porosimetry, was shown to decrease as HEMA
content was incrementally increased.