omation mbers - Society for Laboratory Automation and Screening

TP054
Andreas Kuoni
University of Neuchatel
Institute of Microtechnology
Rue Jaquet-Droz7
Neuchatel, CH – 2007 Switzerland
Andreas.Kuoni@unine.ch
Multi-Channel Dispenser for Micro-Array Printing
173
Co-Author(s)
Marc Boillat, University of Neuchatel
Bart van der Schoot, Seyonic SA
The ability to carry out assays in parallel in a micro array format is essential for the success of high throughput
analysis systems. We report a new type of piezoelectric dispenser which dispense 65 pL droplets in parallel and
in a 2D array format onto a micro-arrray at a 2D spacing of 500 µm. The dispenser is continuously loaded from a
standard well plate with a well spacing of 4.5 mm during dispensing. An innovative technology using Polyimide
sheets has been developed for the construction of a 2D micro-array printing device. The flexibility of the laminated
Polyimide sheets allows assembling different sizes of dispensing arrays at a center-to-center spacing of 500 µm
in a modular way by stacking flexible dispenser sheets (e.g., 32*48, 16*24, 8*12). The major advantages of the
present device are no interface changes in the fluidic path, no tubing for liquid transportation, and no robotics
system. The spotting has been characterized qualitatively and quantitatively. First we observe satellite free
dispensing. The average droplet velocity is 1.79 m/s ± 0.2 m/s per line at a common driving signal of 180 Volts.
The dispensed droplet has a nominal volume of 65 pL, calculated from the droplet diameter. The variation of
dispensed volume between spotting sites is have been compared first by calculating the evaporation rate and
then applying this to the droplet evaporation time. The average evaporation time for a single channel measuring 30
successive droplets is t=1.40 sec ± 0.12 sec results in a CV value of 8.6 %.
TP055
Fred Lange
University of Rostock
Institute of Automation
R.-Wagner-Str. 31
Rostock18119 Germany
fred.lange@uni-rostock.de
Data Mining and Visualization in Sports Medicine Automation by WebServer Oriented
Software and WebBrowser Oriented User Interfaces
Co-Author(s)
Regina Stoll
Reinhard Vilbrandt
Laboratory data archives organized in databases play an important role in modern preventive medicine. Especially
in Occupational Health and Sports Medicine huge amounts of data about human’s fitness are produced.
Distributed data sources and acquisition systems do not allow to produce enough representive data about all
possible populations in every lab. So, the idea was born to centralize databases and to identify physical fitness
data by methods and algorithms on the fundaments of these centralized data. But, so the concept for data
processing in data mining has to be centralized too. The strategy for this is based on Web-server running software
components. The goal was to prevent local software components, except the operating system. This would be
a big step in direction of ubiquitous computing – independed from the operating system. The problem’s solution
is oriented to build data mining procedures in graphic object oriented software systems. Virtual instruments (VIs)
in LabVIEW (National Instruments) are an excellent tool for interpretation and visualization procedures for data in
Occupational and Sports Medicine. In the last five years many applications for data processing in Occupational
and Sports Medicine have been developed in our lab based on local Virtual Instruments connected to real sensor
systems. A way to solve the problem in general is an implementation of centralized server oriented operated
LabVIEW applications connected to a Man-Machine-Interface performed by a Web-Browser. The paper shows
some model-applications and the way to realize Web-Interfaces and Data mining with the Internet-Toolkit in
LabVIEW. The system can be used to fill the database with data from collaborators via Internet and get them back
the calculated results as an image or as an ASCII-data set.
POSTER ABSTRACTS