omation mbers - Society for Laboratory Automation and Screening

TP045
Jennifer Halcome
Eppendorf-5 Prime, Inc.
Product Applications
6135 Gunbarrel Avenue, Suite 230
Boulder, Colorado 80301
jhalcome@5prime.com
168
Co-Author(s)
George Halley
Gerry Huitt
Plasmid DNA Purification Using the Eppendorf Perfectprep ® Plasmid 96 Vac Direct Bind Kit
on the Eppendorf epMotion 5075 Workstation
The Eppendorf epMotion 5075 workstation allows for full automation of a variety of applications. It is particularly
suitable for the rapid purification of 96 plasmid preparations using the Eppendorf Perfectprep Plasmid 96 Vac
Direct Bind kit. The protocol for this kit is provided, pre-programmed, with the epMotion 5075 workstation and
results in excellent yields of high quality plasmid DNA. When sequenced, the DNA exhibits high passing rates
and exceptional Phred Q>20 quality scores. The Eppendorf Perfectprep Plasmid 96 Vac Direct Bind kit and
the epMotion 5075 provide a complete system for low and medium throughput isolation of plasmid DNA. The
epMotion 5075 can be purchased with a variety of deck configurations that can include up to three temperaturecontrolled
positions. In addition, the deck may incorporate either a vacuum manifold or a thermocycler. The
versatility of the epMotion 5075 allows for the processing of a variety of applications including purification of BAC
DNA using the Eppendorf Perfectprep BAC 96 kit and purification of PCR products using the Eppendorf PCR
Cleanup 96 kit. Other applications include PCR and sequencing setup, thermocycling and restriction digests.
TP046
Paul Held
Bio-Tek Instruments
100 Tigan Street
Winooski, Vermont 05404
heldp@biotek.com
Co-Author(s)
Lenore Buehrer
The ELx405 HT 384-Well Microplate Washer: Designed to Meet the Rigors of Biomolecular
Screening
In today’s HTS environment, 384-well microplates have become the preferred format, regardless of the screening
assay type. Most often these assays require some sort of aspiration and/or dispensing of fluids to the wells of
the microplate typically accomplished by a microplate washer. The ELx405 HT is a dedicated 384-well microplate
washer that utilizes 192 pairs of aspiration and dispense-tubes to provide rapid and effective aspiration and
dispensing of fluid without sacrificing features or performance. Using the same patented dual-action manifold
design as the industry standard ELx405 Select washer, the ELx405 HT allows independent control, both vertically
and horizontally, of the aspiration and the dispense manifolds. Several standard features have been included
to make the ELx405 washer robotic-friendly and ideal for automation. Vacuum detection is provided to ensure
that the vacuum pump is switched on and vacuum vessels are connected. Flow protection alerts the user to an
interruption of flow to the dispensing manifold. Waste-level detection prevents overflow of waste into the vacuum
pump. In addition to an overview of the ELx405 HT washer, data demonstrating the washer’s accuracy and
precision of fluid-dispense, evacuation efficiency, as well as speed and timing will be included
.