omation mbers - Society for Laboratory Automation and Screening

TP041
Joseph Granchelli
NalgeNunc International
Research and Design
75 Panorama Creek Drive
Rochester, New York 14260
jgranch1@rochester.rr.com
Anaylsis of Nucleic Acid and Protein Arrays on NUNC ArrayCote 16-Well Slides and
96-Well Plates
166
Co-Author(s)
Dan Schroen
Tom Cummins
Microarray technology, with its ability to produce a simultaneous profile of gene expression across tens-ofthousands
of transcripts, is an extremely powerful technique in genetic analysis. However, given the high density
and therefore large expense associated with high-density printed arrays and target material, sample replication
is often limited for most researchers. Often, many thousands of the measured transcripts on a high density,
slide- based array are not regulated under the experimental conditions of interest and add only expense rather
than understanding. The first step in a series of experiments often involves determining the subset of regulated
transcripts using high-density arrays. Smaller arrays can be used that cover the subset of transcripts found to
be regulated under a given set of experimental conditions.These smaller, focused arrays offer a significant cost
advantage, allowing a trade between the expense of high density and the statistical power from multiple replicates.
By combining the replicate sampling possible in a chambered format with chemistry (APS, Epoxy, and Aldehyde)
familiar to microarray users, Nunc has extended array technology in the form of 96-well plate and 16-well slide
array platforms with excellent performance characteristics. With this format it is possible to spot either nucleic
acids or proteins with excellent spot morphology (protein spots less than 250µm, PCR product spots less than
120µm) and good binding capacity (saturated signals at less than 0.2µg/ml probe concentration). Background
levels are low, yielding substantial signal/noise ratios.
TP042
David Griffin
Genevac, Inc.
711 Executive Boulevard, Suite H
Valley Cottage, New York 10989
dgriffin@genevacusa.com
Combining Lyophilisation and Centrifugal Evaporation to Make a Fast Drying Process That
Results in Rapidly Resuspendable Dry Solid Compound
Centrifugal Evaporation is the solvent removal process of choice for high speed parallel drying of large numbers
of solutions in the 0.1 to 50 mls volume range, (particularly preparative HPLC fractions). However, lyophilisation is
sometimes preferred because the final dried product may resuspend more easily and is less likely to adhere to the
original vessel. Lyophilisation is however a very slow process taking as much as two days, unless a labor intensive
“shell freezing” process is employed. Genevac have developed a hybrid process, which gives a lyophilised end
product but is three-four times faster than conventional lyophilisation (i.e., possible overnight). A specially modified
centrifugal evaporator is used, and racks can be handled straight from a fraction collector to the machine without
the need for individual tube handling.