omation mbers - Society for Laboratory Automation and Screening

TP021
Cristopher Cowan
Promega Corporation
Applications
2800 Woods Hollow Road
Madison, Wisconsin 53711
ccowan@promega.com
156
Co-Author(s)
James Batchelor, Zymark Corporation
Automated Isolation of Nucleic Acids on the Zymark SciClone ALH 3000 Using Promega’s SV
96 Nucleic Acid Isolation Chemistries
Isolation of nucleic acids is central to all manipulations used in molecular biology. Promega offers a variety of
SV 96 Nucleic Acid Isolation Systems which provide a coherent set of vacuum based isolation protocols for
purification of genomic DNA from tissues and tissue culture cells, total RNA from tissues and tissue culture cells,
plasmids from bacterial cell cultures and PCR clean-up from PCR reactions. In collaboration with Zymark we
have developed methods for the purification of all of these nucleic acid types using Promega’s SV 96 Nucleic
Acid Isolation Systems on the Zymark SciClone ALH 3000 liquid handling workstation. The SV 96 Nucleic Acid
Isolation Systems use filter plates for binding nucleic acids. Instead of using vacuum filtration for binding and
washing steps, the Zymark SciClone ALH 3000 liquid handling workstation uses a positive pressure system to
push reagents through the filter plates. Once bound, the nucleic acids are washed and the highly purified nucleic
acid products are eluted in water. Once isolated, the samples can be used directly for downstream PCR, RT-PCR,
quantitative PCR, sequencing and other molecular biological reactions. We demonstrate the automated isolation of
a variety of nucleic acid types (genomic DNA, total RNA, plasmid, PCR product) from tissues, tissue cell cultures,
bacterial cultures, and PCR reactions. Performance of the isolated nucleic acids in PCR, RT-PCR, and sequencing
is demonstrated, with no detectable cross contamination.
TP022
Carole Crittenden
Molecular Devices Corporation
1311 Orleans Drive
Sunnyvale, California 94089
Carole_Crittenden@moldev.com
Demonstration of Mitochondrial Aequorin Luminescent Signal Measurement by FLIPR3
Co-Author(s)
Jennifer McKie
Yan Zhang
The FLIPR3 ® is a cell-based HTS system that measures intracellular fluorescence assays such as calcium flux
and membrane potential. Using a sensitive camera, the high throughput capabilities of the FLIPR3 system are
expanded to include measurement of aequorin luminescent signal in cell-based G Protein Coupled Receptor
Assays for drug discovery.FLIPR3 simultaneously reads all wells throughout the reagent addition process.
The ability to read immediately after dispensing enables aequorin that yield flash luminescence. In this study,
we demonstrate luminescence measurement capabilities for FLIPR3 and evaluate cell based luminescence
applications for drug discovery.