omation mbers - Society for Laboratory Automation and Screening
omation mbers - Society for Laboratory Automation and Screening
omation mbers - Society for Laboratory Automation and Screening
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TP015<br />
Jesse Campbell<br />
Applied Molecular Evolution<br />
3520 Dunhill Street<br />
San Diego, Cali<strong>for</strong>nia 92121<br />
jcampbell@amevolution.com<br />
153<br />
Co-Author(s)<br />
Michael J. Cohen, Andrew Korytko,<br />
Barbara Swanson, Alain P. Vasserot<br />
Development of a Data-driven, Integrated Aut<strong>omation</strong> Plat<strong>for</strong>m <strong>for</strong> High Throughput<br />
Expression <strong>and</strong> <strong>Screening</strong> of Protein Engineering Libraries<br />
Protein engineering can drastically impact the efficacy <strong>and</strong> safety profile of therapeutic proteins <strong>and</strong> exp<strong>and</strong> their<br />
clinical utility. The systematic exploration of beneficial amino acid changes in a protein requires the creation,<br />
expression, <strong>and</strong> screening of mutational libraries <strong>and</strong> the use of a wide range of procedures <strong>and</strong> assays. In<br />
order to facilitate the rapid evaluation of these libraries, we modified a Biomek FX-based system to create an<br />
integrated aut<strong>omation</strong> plat<strong>for</strong>m that enables robotic h<strong>and</strong>ling of engineering projects with widely different goals<br />
<strong>and</strong> requirements. This system is now capable of automated colony picking, plasmid preparation, mammalian<br />
cell transfection, quantification <strong>and</strong> robotic execution of a variety of project-specific screening assays. Integrated<br />
analysis software allows data-driven normalization of expression levels <strong>and</strong> sample re-arraying as well as the<br />
selection of primary leads based upon real-time investigator input. The heavy use of custom-designed hardware<br />
<strong>and</strong> software solutions was necessary to design a plat<strong>for</strong>m that considerably exp<strong>and</strong>s the utility of the original core<br />
system by maximizing unattended overnight usage <strong>and</strong> by accommodating a wide variety of libraries, expression<br />
systems, <strong>and</strong> screening assays.<br />
TP016<br />
Julie Chang<br />
MDL In<strong>for</strong>mation Systems<br />
14600 Catalina Street<br />
San Le<strong>and</strong>ro, Cali<strong>for</strong>nia 94577<br />
jchang@mdl.com<br />
An Inventory Management Solution From Registration to Assays<br />
The compound management group is often described as the hub of activity that manages the <strong>for</strong>mulation,<br />
storage, distribution, <strong>and</strong> job tracking of requests <strong>for</strong> plates to various groups that input compounds as well as<br />
request compounds <strong>for</strong> testing. MDL will present an in<strong>for</strong>mation management solution that will accommodate the<br />
workflows of chemists as well as biologists in the new MDL Plate Manager.<br />
POSTER ABSTRACTS
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