omation mbers - Society for Laboratory Automation and Screening
omation mbers - Society for Laboratory Automation and Screening
omation mbers - Society for Laboratory Automation and Screening
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TP003<br />
Edward Alderman<br />
Zymark Corporation<br />
Drug Discovery Consulting<br />
Zymark Center - 68 Elm Street<br />
Hopkinton, Massachusetts 01748<br />
ed.alderman@zymark.com<br />
Co-Author(s)<br />
Geoffrey N. Grove, Zymark Corporation<br />
Mei Cong <strong>and</strong> Zhong Zhong, Cell & Molecular Technologies<br />
Chris Cowan, Promega<br />
Casey Laris, Q3DM<br />
Aut<strong>omation</strong> of Apoptosis <strong>and</strong> Reporter-Gene Assays Using Division-Arrested<br />
NFkB HEK293 Cells<br />
Due to the high failure rate of NCE’s in animal trials, researchers are increasingly choosing to run assays in more<br />
physiologically relevant systems. This has given rise to an increasing dem<strong>and</strong> <strong>for</strong> cell-based assays earlier in drug<br />
discovery <strong>and</strong> development work. We demonstrate that a cell-culture facility is not needed to per<strong>for</strong>m automated<br />
cell-based assays. Using the Staccato Sciclone Cell Station-Assay (an automated cell-based assay system) <strong>and</strong><br />
division arrested HEK293 cells provided by CMT (Cell & Molecular Technologies), we present data demonstrating<br />
the equivalency of division-arrested cells to normal cells when assayed using Promega’s Apo-ONE Homogenous<br />
Caspase-3/7 <strong>and</strong> Steady-Glo ® Luciferase Assay Systems.<br />
TP004<br />
Chris Barbagallo<br />
Millipore Corporation<br />
Research & Development<br />
17 Cherry Hill Drive<br />
Danvers, Massachusetts 01923<br />
chris_barbagallo@millipore.com<br />
Automating Aqueous Compound Solubility <strong>Screening</strong><br />
147<br />
Co-Author(s)<br />
Greg Kazan<br />
Libby Kellard<br />
Jason Blodgett<br />
Alan Weiss<br />
Compound solubility characterization in the early stages of the drug discovery process is becoming an essential<br />
tool prior to per<strong>for</strong>ming any biological testing. The main reason <strong>for</strong> this being that low solubility can lead to<br />
unreliable results during in vitro studies, such as the generation of false positives in these bioassays. This wastes<br />
valuable time <strong>and</strong> resources, which can add significant cost to drug research projects. In addition to these<br />
factors, the st<strong>and</strong>ard shake-flask method now used to evaluate drug solubility is inherently low throughput <strong>and</strong><br />
labor intensive. Millipore has developed a 96-well, filter-based method <strong>for</strong> both semi-quantitative <strong>and</strong> quantitative<br />
solubility determinations. We describe the use of these protocols on several liquid h<strong>and</strong>ling plat<strong>for</strong>ms to show the<br />
ease <strong>and</strong> robustness of full aut<strong>omation</strong> of this process. Results shown correlate well with values obtained using<br />
the shake-flask method. Depending on calibration <strong>and</strong> replicate number, this procedure is capable of generating<br />
results <strong>for</strong> hundreds of samples per day.<br />
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