omation mbers - Society for Laboratory Automation and Screening

TP001
Thor Anders Aarhaug
SINTEF
Materials Technology
Sem Sælandsvei 12
TrondheimNO-7465 Norway
taarhaug@ntnu.no
Sintalyzer – A Fully Automated Analytical System for Fluoride Analysis
146
Co-Author(s)
Kalman Nagy
In the aluminum industry, fluorine analyses are interfered by the presence of aluminum, silicon and iron. Performing
standard fluorine analyses using TISAB buffers will result in a negative analytical error due to complexation of
fluoride. The Sintalyzer analytical system is a customized system for fluoride analysis in the aluminum industry. It
is used exclusively in the Scandinavian countries, and has also been installed in Germany, India, and Slovakia. The
system uses several buffers that, in addition to proper sample preparation, render the fluoride electrochemically
measurable. For optimal analytical performance, a standard addition technique is used. To avoid a dilution of the
original matrix, micro volumes of highly concentrated standard is used. Since the concentration-voltage relation is
given by the Nernst equation, the addition volumes have to be calculated dynamically in order to ensure an equal
distance between the responses from the fluoride electrode. Although there are alternatives to potentiometry for
fluoride analysis in the aluminum industry, the investment cost of, for example an XRF, are 10 times higher and
requires by far more maintenance. Also, the sensitivity of the ion-selective electrode yields a better analytical
performance. The Sintalyzer analytical system utilizes a standard addition technique that, by changing the ionselective
electrode, can be extended to several other ions. By applying a lead ion-selective electrode, sulphur
dioxide can be determined by titration with lead nitrate. The system comes with complete procedures for the
analysis of fluoride of all matrices that are common in aluminum industry. The system is accredited according to
Norwegian Standard quality assurance of GLP.
TP002
Monica Adams
Amersham Biosciences
Development Integration/Genomics
800 Centennial Avenue
Piscataway, New Jersey 08854
monica.adams@amersham.com
The Automation of TempliPhi and GenomiPhi on the Tecan Genesis Freedom
The Genesis Freedom Automation Workstation from Tecan offers you a simple, effective way to gain all the
advantages of liquid handling and laboratory automation, which was used to automate Amersham Bioscience’s
TempliPhi and GenomiPhi kits. The Tecan Gemini software system allows easy protocol set-up and operation
of the Freedom workstation. Amersham Bioscience’s TempliPhi Amplification kit is a novel process to efficiently
prepare DNA sequencing templates in 4 – 6 hours. Templates are prepared by rolling circle amplification using
bacteriophage Phi29 DNA polymerase.The GenomiPhi kit utilizes Phi29 DNA polymerase enzyme to exponentially
amplify single and double stranded DNA by strand displacement amplification. The Tecan Freedom, with an eightchannel
liquid handling arm, is capable of generating multiple TempliPhi or GenomiPhi 96-well reaction plates in
more than half the time of manual pipetting. TempliPhi and GenomiPhi can be easily automated to work with liquid
handling systems.