omation mbers - Society for Laboratory Automation and Screening

4:00 pm Wednesday, February 4 Emerging Technologies – Automation Systems Room A3
Sanjaya Joshi
Userspace Corporation
11118 NE 141 Place
Kirkland, Washington 98034
sanjay@userspace.com
An Automated Flow Cytometry Quality Protocol and Workflow System for Managing
Instruments, Samples, and Data
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Co-Author(s)
Michael R. Loken
Hematologics, Inc.
Flow Cytometry is gaining significance outside the research laboratory as a high-resolution clinical tool for the
detection, classification, staging, and recurrence of Hematologic neoplasms. Intensity relationships between
antigens is proving to be an important discriminator of normal and aberrant hematopoietic cells. Userspace
Corporation and HematoLogics, Inc. have been collaborating to create a web-service based real-time workflow
system combined with a unique performance assessment for the instrument calibration based on invariant
biological markers. A high resolution instrument validation protocol can be established using lymphocyte CD4
fluorescence intensity. In a study of normal adult blood using these QC procedures, the intensity of CD4 on
lymphocytes was found to be essentially invariant for 21 individuals assayed on the two instruments collected
over a period of 8 months. These results demonstrate that in a data space with a dynamic range of 4 decades, the
biological variation of the mean intensity from individual to individual for this one antigen is less than the variability
of expression within the individual. The biological expression of this antigen becomes an independent biological
standard. This QC procedure is a rapid means of assessing instrument performance. This quality program
combined with the tracking and audit of samples and patient records securely in real-time allows both clinical
laboratories and research laboratories conducting clinical trials to remotely track and analyze the FCS data format
(converted into XML for data stream based interpretation, analysis and report generation). Templates can be built
around this system for various quality and regulatory standards.
4:15 pm Wednesday, February 4 Emerging Technologies – Automation Systems Room A3
Emir Osmanagic
Scinomix
4069 Wedgeway Court
Earth City, Missouri 63045
eosmanagic@scinomix.com
Vertical Integration Platform
Co-Author(s)
Russell Leeker
Traditionally, companies have designed laboratory automation systems to operate with few significant changes
during assay production. The inflexibility of this approach is the primary reason companies are supporting
development of more versatile systems. The Vertical Laboratory Integration Platform (VIP) is a framework for
distributed modular automation for laboratory processes. Modularity of the components, highly flexible product
transport mechanisms, and a high level of distributed intelligence are key characteristics of the VIP. Laboratories
can realize an excellent return on investment through the VIP’s ability to adapt to the quick and rapid changes of
lab processes. The first version of the VIP has been developed for Cell-Based Assays incorporating incubation,
liquid handling, washing and plate sealing on plug-and-play shelves under environmental controlled conditions.
The system transports any height SBS standard footprint plates to and from instruments in a vertical configuration,
saving valuable laboratory space. When a process changes and an additional or different instrument is required a
plug and play shelf can be removed and reconfigured then plugged back into the VIP framework.
PODIUM ABSTRACTS