omation mbers - Society for Laboratory Automation and Screening

11:00 am Wednesday, February 4 Emerging Technologies – Hardware Room A3
Brian Cunningham
SRU Biosystems
14A Gill Street
Woburn, Massachusetts 01801
bcunningham@srubiosystems.com
Label-Free Assays Using the BIND System
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Co-Author(s)
Peter Li, Stephen Schulz,
Bo Lin, Cheryl Baird
Screening of biochemical interactions becomes simpler, less expensive, and more accurate when labels, such as
fluorescent dyes, radioactive markers, and colorimetric reactions, are not required to quantify detected material.
SRU Biosystems has developed a biosensor technology that is manufactured on continuous sheets of plastic
film, and incorporated into standard microtiter plates and microarray slides to enable label-free assays to be
performed with high throughput, high sensitivity, and low cost per assay. The biosensor incorporates a narrowband
reflectance filter, in which the reflected color is modulated by the attachment/detachment of biochemical material
to the surface. The technology offers 4-orders of linear dynamic range, and uniformity within a plate with a
coefficient of variation of 2.5%. Two readout instruments are demonstrated: a plate reader capable of reading one
data point per well in 96- or 384-well microplates, and a high performance imaging plate reader. Both instruments
are capable of reading an entire biosensor plate in 15-30 seconds and integrating with robotic microplate
handlers. Using conventional biochemical immobilization surface chemistries, a wide range of assay applications
are enabled. Small molecule screening, cell proliferation/cytotoxicity, enzyme activity screening, protein-protein
interaction, cell membrane receptor, and microarray imaging are among the applications demonstrated.
11:15 am Wednesday, February 4 Emerging Technologies – Hardware Room A3
Martin Dufva
Mikroelektronik Centret
Technical University of Denmark
Building 345 East
Kongens Lyngby, Denmark
mdu@mic.dtu.dk
Co-Author(s)
Michael Stangegaard, Per Jensen Mikkelsen,
Louise Dahl Christensen, Claus BV Christensen
A Flexible Substrate for DNA Microarray Hybridization and Detection Using a
Business Card Scanner
DNA and protein microarrays are powerful tools for both protein and mRNA expression profiling, respectively, due
to the immense parallelism of the analysis methods. Microarrays can furthermore be used for diagnosing bacteria
and quantifying small molecule contaminations, like pesticides in water, with increased sensitivity compared
to traditional methods. Traditional microarray technology, however, often relies on bulky equipment. Here we
present a flexible (100 micrometer thin) microarray substrate that can be used for detecting microarray-analyte
interactions using an ordinary business card scanner for signal generation. Hybridized DNA was stained by a
color reaction mediated by alkaline phosphatase prior to scanning. The results were comparable with the results
obtained using a desktop scanner and showed that hybridization of 50 pM target could be detected using this
alternative detection system. Detection of immobilized biotinylated DNA on the microarray surface indicated that
2–3 amol of biotinylated DNA could be detected. The business card scanner (weight approx. 200 gram) only needs
an USB port on a portable computer and no AC power to function, making this detection system truly portable.
Furthermore, the thin and flexible microarray substrate may be a more appropriate solid support than glass for
integrating microarrays with microfluidics.
PODIUM ABSTRACTS