omation mbers - Society for Laboratory Automation and Screening

8:30 am Thursday, February 5 Genomics – Arrays Room A1
Jon Chudyk
Marshfield Clinic Research Foundation
1000 N. Oak Avenue ML7
Marshfield, Wisconsin 54449
chudykj@cmg.mfldclin.edu
Co-Author(s)
Terry L. Rusch, Kim Fieweger, William Dickinson, Jian Che
Marshfield Clinic Research Foundation
104
Mitchel J. Doktycz, Adong Yu, James L. Weber
Oak Ridge National Laboratory
Microtape and Associated Instrumentation for Continuous Array High Throughput
Genotyping
Lower cost, higher throughput genotyping is a common goal among genetics labs. Automation has greatly
increased over the years, but still requires the manipulation of numerous microtiter plates. Our laboratory is using
a continuous reel of 384 well arrays on polypropylene tape (microtape) to genotype diallelic polymorphisms.
Genotyping costs are lowered through more efficient automation of the microtape as compared with microtiter
plate handling, as well as a reduction in reagent costs by decreasing reaction volumes. Our current version of
microtape has wells with reaction volumes of 700 nanoliters or less. The diallelic polymorphisms are typed by
tagging allele-specific PCR primers with FAM or JOE molecular beacon uniprimers. Both short insertion/deletion
and base substitution (SNP) polymorphisms have been successfully typed in the microtape using this assay.
Commercial equipment for the microtape is not presently available, therefore a series of instruments to handle the
tape were developed in-house. A pipetting instrument was developed to deliver specific DNA samples or other
reagents. A solenoid micro-valve aspirating and jetting unit was developed for dispensing a common reaction
mix. The arrays are sealed with commercially available continuous roll seal material prior to polymerase chain
reaction (PCR). PCR is performed in a waterbath-based thermal cycler. After PCR the arrays are read using
an epi-fluorescence detection unit. The reader uses either an argon ion or solid state laser for excitation and
photomultiplier tubes (PMTs) for detection. The results are analyzed using software written in-house.
9:00 am Thursday, February 5 Genomics – Arrays Room A1
Andrey Ghindilis
CombiMatrix Corporation
6500 Harbour Heights Parkway Suite 301
Mukilteo, Washington 98275
aghindilis@combimatrix.com
Immunoassays and Sequence-Specific DNA Detection on a Microchip Using Enzyme
Amplified Electrochemical Detection
Co-Author(s)
Kilion Dill
Kevin Schwarzkopf
A CMOS fabricated silicon microchip was used as a platform for immunoassays and gene expression studies
utilizing CombiMatrix’s unique DNA synthesis methodology. The chip is covered with a biofriendly matrix wherein
the chemistries occur. The active silicon chip has over 1,000 active electrodes that can be individually addressed
for both synthesis of DNA and protein attachment to a membrane on the chip surface. The active chip can be
further used for the analysis of gene expression targets and detection of various analytes using antibody selfassembly
immunoassay techniques.