omation mbers - Society for Laboratory Automation and Screening

9:30 am Wednesday, February 4 Genomics – Instrumentation Room A1
Ram Vairavan
AutoGenomics
2270-K Camino Vida Roble
Carlsbad, California 92009
rvairavan@autogenomics.com
A New Dimension in Routine Genomic and Proteomic Analyses
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Co-Author(s)
Fareed Kureshy
Vijay Mahant
The INFINITI System is an automated multiplexing platform for genomic and proteomic analysis that delivers
sample to results without manual intervention. Utilizing a novel thin film matrix as the microarray, disease
specific probes are spotted on the surface and loaded into magazines. The INFINITI Analyzer integrates all
the discrete processes of sample handling, reagent management, hybridization and detection for the analyses
of DNA and proteins in a totally self-contained system. The “open architecture” design enables adaptation of
multiple methodologies such as hybridization assay, primer extension assay, competitive and sandwich format
immunoassays to perform Single Nucleotide Polymorphisms (SNPs), Short Tandem Repeats (STRs), micro-satellite
analysis, gene expression analysis and protein determinations. The entire process is managed by the proprietary,
Qmatic operating software which integrates all the complex processes in DNA analyses from amplified sample
to test result without any operator intervention. All supplementary reagents are contained in the Intellipac reagent
module with an on-board microchip that efficiently manages the reagents, electronically recording all relevant
reagent specific information, such as expiry date, tracking reagent usage and the assay protocol for the specific
test. The microarray is read in the built-in confocal microscope with results analyzed and presented in a graphical
format for easy interpretation. The used chips are discarded in the waste drawer. The system can process 24
microarrays simultaneously. Designed to operate on a “Load and Go” concept current applications include Cystic
Fibrosis, Bleeding disorder tests, Th1/Th2 immune response test. Future applications include comprehensive
profiles of specific cancers with DNA, protein and methylation markers.
10:30 am Wednesday, February 4 Genomics – SNPs Room A1
Keith Roby
Beckman Coulter, Inc.
4300 N. Harbor Boulevard
Fullerton, California 92834
kwroby@beckman.com
Co-Author(s)
Laura Pajak, Dana Campbell,
Zhiming Jiang, Chad Pittman,
Scott Boyer
Automation of Amplification and Primer Extension Chemistries for Beckman Coulter’s
SNPstream ® Genotyping System
The information included in this presentation describes the utilization of the Biomek ® FX Laboratory Automation
Workstation for the process of SNP scoring using the SNPstream Genotyping System and assay reagents. Pre-
and post-PCR methods are executed on separate instruments to ensure that the overall hygiene of the system is
maintained thereby minimizing the possibility of cross-contamination. Pre-PCR methods include Genomic DNA
Redistribution, and Multiplex Reaction Setup. Post-PCR methods include Exo/-SAP Cleanup, Multiplex Single
Base Primer Extension and Hybridization of the tagged extension products to the SNPstream assay plate. The
multiplex PCR and Primer extension protocols currently can accommodate up to 12 targets per reaction (i.e., a
12-plex reaction). Components required to generate amplicons in a multiplex reaction and to process these targets
with SNPware ® Reagent Kits will be described; this includes:
• Describing the Biomek FX configurations utilized for pre- and post-PCR methods
• Describing the important features of the methods used to generate and process samples
• Describing results obtained when using these methods.
Using the methodology described here, processing of 8 plates (>36,000 SNPs) can be achieved in approximately
6 hours.
PODIUM ABSTRACTS