5th EuropEan MolEcular IMagIng MEEtIng - ESMI

5th EuropEan MolEcular IMagIng MEEtIng – EMIM2010
Point-of-Care Microscopy: Molecular Imaging with Cellular Resolution
Contag C.H. .
Division of Neonatal and Developmental Medicine, and a member of the BioX faculty at Stanford University.
ccontag@stanford.edu
Micro-optical designs are enabling the development
of miniaturized microscopes that can reach inside
the body to interrogate disease states microscopically.
This is leading to an emerging field of in vivo
pathology that is changing the diagnostic paradigm
from biopsy
and conventional
histopathology to
one of point-ofcare
histopathology
coupled with telepathology.
These
advances are closing
the gap between
micro–mirror
the patient and the
pathologist and
have the potential of accelerating diagnosis and
guiding therapy. While recent advances in this
field have been significant, many issues must be
resolved before this clinical transformation may be
fully realized. There are technological and translational
advances that are driving this field and are
leading towards in vivo microscopy becoming a standard
clinical tool. By removing the spatiotemporal
separation between the pathologist and patient, we
can accelerate clinical diagnosis and advance clinical
care for patients with a wide variety of diseases.
References
1. Liu, JTC, Mandella, MJ, Loewke, NO, Haeberle, H, Ra, H,
Piyawattanametha, W, Solgaard, O, Kino, GS, Contag,
CH (2010) Micromirror-scanned dual-axis confocal
microscope utilizing a gradient-index relay lens for
image guidance during brain surgery. JBO. In Press.
2. Piyawattabanetha, W, Ra, H, Mandella, MJ, Loewke,
Wang, TD, Kino, GS, Solgaard, O, Contag, CH (2009) 3-D
near-infrared fluorescence imaging using a MEMSbased
miniatuire dual axis confocal microscope. IEEE J.
Sel. Topics Quantum Electronics. 15(5): 1344-1350.
3. Mackanos, MA, Hargrove, J, Wolters, R, Du, CB,
Friedland, S, Soetikno, RM, Contag, CH, Arroyo, MR,
Crawford, JM, Wang, TD (2009) Use of an endoscopecompatible
probe to detect colonic dysplasia with
Fourier transform infrared spectroscopy. J Biomed
Optics 14, 044006. PMID: 19725718
4. Liu, JT, Mandella, MJ, Crawford, JM, Contag, CH, Wang,
TD, Kino, GS. (2008) Efficient rejection of scattered light
enables deep optical sectioning in turbid media with
low-numerical-aperture optics in a dual-axis confocal
architecture. J Biomed Opt. 13(3):034020.
5. Hsiung, P-L, Hardy, JW, Friedland, S, Soetikno, R, Du, CB,
Wu, APW, Sahbaie, P, Crawford, JM, Lowe, AW, Contag,
CH, Wang, TD. (2008) Detection of colonic dysplasia in
vivo using a targeted fluorescent septapeptide and
confocal microendoscopy. Nat. Med. 14(4): 454-8.
6. Wang, TD, Triadafilopoulos, G, Crawford, JM, Dixon, LR,
Bhandari, T, Sahbaie, P, Friedland, S, Soetikno, R, Contag,
CH. (2007) Detection of Endogenous Biomolecules
in Barrett’s Esophagus by Fourier Transform Infrared
Spectroscopy. Proc. Natl. Sci, USA. 104(40): 15864-9.
EuropEan SocIEty for MolEcular IMagIng – ESMI
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Inaugural Lecture by Christopher H. Contag