Klaas Martinus Pos Publications 2006-2012 - CEF-MC

Klaas Martinus Pos
Publications 2006-2012
(last updated 09/2012)
Bohnert JA, Schuster S, Seeger MA, Fahnrich E, Pos KM, Kern WV (2008) Site-directed mutagenesis
reveals putative substrate binding residues in the Escherichia coli RND efflux pump AcrB. J Bacteriol
190:8225-8229
Brandstatter L, Sokolova L, Eicher T, Seeger MA, Briand C, Cha HJ, Cernescu M, Bohnert J, Kern
WV, Brutschy B, Pos KM (2011) Analysis of AcrB and AcrB/DARPin ligand complexes by LILBID MS.
BBA-Biomembranes 1808:2189-2196
Camargo SMR, Singer D, Makrides V, Huggel K, Pos KM, Wagner CA, Kuba K, Danilczyk U, Skovby
F, Kleta R, Penninger JM, Verrey F (2009) Tissue-specific amino acid transporter partners ACE2 and
collectrin differentially interact with hartnup mutations. Gastroenterology 136:872-882
Eicher T, Cha HJ, Seeger MA, Brandstatter L, El-Delik J, Bohnert JA, Kern WV, Verrey F, Grutter MG,
Diederichs K, Pos KM (2012) Transport of drugs by the multidrug transporter AcrB involves an access
and a deep binding pocket that are separated by a switch-loop. P Natl Acad Sci USA 109:5687-92
Furrer EM, Ronchetti MF, Verrey F, Pos KM (2007) Functional characterization of a NapA Na+/H+
antiporter from Thermus thermophilus. FEBS Lett 581:572-578
Gauntlett JC, Gebhard S, Keis S, Manson JM, Pos KM, Cook GM (2008) Molecular analysis of BcrR,
a membrane-bound bacitracin sensor and DNA-binding protein from Enterococcus faecalis. J Biol
Chem 283:8591-8600
Oberfeld B, Ruffieux-Daidie D, Vitagliano JJ, Pos KM, Verrey F, Staub O (2011) Ubiquitin-specific
protease 2-45 (Usp2-45) binds to epithelial Na(+) channel (ENaC)-ubiquitylating enzyme Nedd4-2. Am
J Physiol-Renal Physiol 301:F189-F196
Oswald C, Pos KM (2011) Drug resistance: A periplasmic ménage à trois. Chem Biol 18:405-407
Pietras Z, Lin HT, Surade S, Luisi B, Slattery O, Pos KM, Moreno A (2010) The use of novel organic
gels and hydrogels in protein crystallization. J Appl Crystallogr 43:58-63
Pos KM (2009) Drug transport mechanism of the AcrB efflux pump. BBA-Proteins Proteom 1794:782-
793
Ramadan T, Camargo SMR, Herzog B, Bordin M, Pos KM, Verrey F (2007) Recycling of aromatic
amino acids via TAT1 allows efflux of neutral amino acids via LAT2-4F2hc exchanger. Pflug Arch Eur
J Phy 454:507-516
Ramadan T, Camargo SMR, Summa V, Hunziker P, Chesnov S, Pos KM, Verrey F (2006) Basolateral
aromatic amino acid transporter TAT1 (Slc16A10) functions as an efflux pathway. J Cell Physiol
206:771-779
Reimer D, Pos KM, Thines M, Grun P, Bode HB (2011) A natural prodrug activation mechanism in
nonribosomal peptide synthesis. Nat Chem Biol 7:888-890
Seeger MA, Diederichs K, Eicher T, Brandstatter L, Schiefner A, Verrey F, Pos KM (2008) The AcrB
efflux pump: Conformational cycling and peristalsis lead to multidrug resistance. Curr Drug Targets
9:729-749
Seeger MA, Schiefner A, Eicher T, Verrey F, Diederichs K, Pos KM (2006) Structural asymmetry of
AcrB trimer suggests a peristaltic pump mechanism. Science 313:1295-1298
Seeger MA, von Ballmoos C, Eicher T, Brandstatter L, Verrey F, Diederichs K, Pos KM (2008)
Engineered disulfide bonds support the functional rotation mechanism of multidrug efflux pump AcrB.
Nat Struct Mol Biol 15:199-205

Seeger MA, von Ballmoos C, Verrey F, Pos KM (2009) Crucial role of Asp408 in the proton
translocation pathway of multidrug transporter AcrB: Evidence from site-directed mutagenesis and
carbodiimide labeling. Biochemistry 48:5801-5812
Vargiu AV, Collu F, Schulz R, Pos KM, Zacharias M, Kleinekathofer U, Ruggerone P (2011) Effect of
the F610A mutation on substrate extrusion in the AcrB transporter: explanation and rationale by
molecular dynamics simulations. J Am Chem Soc 133:10704-10707