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il ' ii - Northern Research Station - USDA Forest Service

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, no Z<br />

Fraction Number<br />

Figure l.-_-BiOactivity of preparative HPLC column fractions in the Amaranthus betacyanin bioassay of an extract of<br />

third instar larvae following separation on HPLC. The results of the bioassay were measured as increases in absor-<br />

' bancy at 542 nm, with the background at 620 nm subtracted (A542-A620), indicating levels ofbetacyanin in<br />

Amaranthus seedlings in response to I/10 aliquots offractions, with the bioactivity of I ng, 3 ng , and 30 ng zeatin<br />

" standards indicated at the right. The retention times of standard zeatin (Z), zeatin riboside (ZR), isopentenyladenosine<br />

(iPA), and isopentenyladenine (iP) are indicated, and the bioactive fractions collected for further purification are<br />

shown in brackets.<br />

166

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